##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AclI AflIII AgeI
Alw44I ApaI ApaLI AscI Asp718I
AspI AviII AvrII BalI BbeI
BciVI BlnI BsiWI BspDI BspLU11I
BsrFI BssSI Bst1107I BstZ17I Cfr10I
ClaI CpoI DraIII DrdI FseI
FspI HpaI KasI KpnI KspI
MluI MluNI MscI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1600 GACGTC GACGT^C
AhdI 143 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1729 AGCGCT AGC^GCT
AspEI 143 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 4655 CACGTG CAC^GTG
BsiEI 1913 CGGCCG CG[GA][CT]^CG
BsmBI 1637 GAGACG CGTCTC(1/5)
Bsp1407I 5756 TGTACA T^GTACA
BsrGI 5756 TGTACA T^GTACA
BssHII 2 GCGCGC G^CGCGC
BstBI 1594 TTCGAA TT^CGAA
BstEII 3309 GGTTACC G^GT[ACGT]ACC
BstPI 3309 GGTTACC G^GT[ACGT]ACC
BstXI 3286 CCATATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1913 CGGCCG C^GGCCG
Eam1105I 143 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1913 CGGCCG C^GGCCG
Eco47III 1729 AGCGCT AGC^GCT
Eco52I 1913 CGGCCG C^GGCCG
EcoNI 2663 CCTGTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3309 GGTTACC G^GT[ACGT]ACC
EcoRI 5356 GAATTC G^AATTC
EcoRV 2397 GATATC GAT^ATC
HaeII 1729 AGCGCT [GA]GCGC^[CT]
HincII 1985 GTTGAC GT[CT]^[GA]AC
HindII 1985 GTTGAC GT[CT]^[GA]AC
HindIII 63 AAGCTT A^AGCTT
MfeI 4996 CAATTG C^AATTG
MunI 4996 CAATTG C^AATTG
NspV 1594 TTCGAA TT^CGAA
PflMI 2782 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4655 CACGTG CAC^GTG
PmeI 989 GTTTAAAC GTTT^AAAC
PmlI 4655 CACGTG CAC^GTG
PpuMI 1738 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 461 ACCAGGT A^CC[AT]GGT
SfuI 1594 TTCGAA TT^CGAA
SpeI 2698 ACTAGT A^CTAGT
SphI 5097 GCATGC GCATG^C
SspBI 5756 TGTACA T^GTACA
SspI 4030 AATATT AAT^ATT
Van91I 2782 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4184 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 949 TCCGGA T^CCGGA
1331 TCCGGA
AflII 911 CTTAAG C^TTAAG
5213 CTTAAG
AocI 313 CCTCAGG CC^T[ACGT]AGG
432 CCTCAGG
AvaI 2132 CCCGGG C^[CT]CG[GA]G
3705 CCCGGG
BanI 212 GGCACC G^G[CT][GA]CC
3095 GGCACC
BbvCI 4229 CCTCAGC CCTCAGC(-5/-2)
3059 GCTGAGG
BcgI 737 GCAACACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1936 GCACCTACATCG
BclI 1883 TGATCA T^GATCA
2179 TGATCA
BfrI 911 CTTAAG C^TTAAG
5213 CTTAAG
BglI 13 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1684 GCCACACAGGC
BglII 1028 AGATCT A^GATCT
3525 AGATCT
BlpI 1312 GCTCAGC GC^T[ACGT]AGC
2706 GCTGAGC
Bpu1102I 1312 GCTCAGC GC^T[ACGT]AGC
2706 GCTGAGC
BsaAI 2169 TACGTG [CT]AC^GT[GA]
4655 CACGTG
BsaI 1790 GAGACC GGTCTC(1/5)
4622 GGTCTC
BsaWI 949 TCCGGA [AT]^CCGG[AT]
1331 TCCGGA
BseAI 949 TCCGGA T^CCGGA
1331 TCCGGA
BsmI 2906 GAATGC GAATGC(1/-1)
3105 GCATTC
BsoBI 2132 CCCGGG C^[CT]CG[GA]G
3705 CCCGGG
BspEI 949 TCCGGA T^CCGGA
1331 TCCGGA
BsrBI 1163 CCGCTC CCGCTC(-3/-3)
370 GAGCGG
BsrDI 5705 CATTGC GCAATG(2/0)
223 GCAATG
Bsu36I 313 CCTCAGG CC^T[ACGT]AGG
432 CCTCAGG
CelII 1312 GCTCAGC GC^T[ACGT]AGC
2706 GCTGAGC
Eco81I 313 CCTCAGG CC^T[ACGT]AGG
432 CCTCAGG
FbaI 1883 TGATCA T^GATCA
2179 TGATCA
MroI 949 TCCGGA T^CCGGA
1331 TCCGGA
NcoI 925 CCATGG C^CATGG
2523 CCATGG
NdeI 4649 CATATG CA^TATG
4853 CATATG
PshAI 2304 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4168 GACCATTGTC
PstI 1845 CTGCAG CTGCA^G
5530 CTGCAG
SapI 896 GAAGAGC GCTCTTC(1/4)
1165 GCTCTTC
SmaI 2132 CCCGGG CCC^GGG
3705 CCCGGG
StuI 317 AGGCCT AGG^CCT
3404 AGGCCT
SwaI 5344 ATTTAAAT ATTT^AAAT
5639 ATTTAAAT
XmaCI 2132 CCCGGG C^CCGGG
3705 CCCGGG
XmaI 2132 CCCGGG C^CCGGG
3705 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BamHI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI