##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AcsI AflII AflIII AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI AvrII BamHI BcgI BciVI
BfrI BglII BlnI BsiWI BsmBI
BspDI BspHI BspLU11I BssHII BstBI
ClaI CpoI DraI EcoRI EcoRV
FseI HindIII MfeI MluI MunI
NdeI NotI NruI NspI NspV
PacI PinAI PmeI PshBI PvuI
RcaI RsrII SalI SfuI SnaBI
SpeI SphI SspI SwaI Tsp509I
XbaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 6444 TCCGGA T^CCGGA
AclI 6027 AACGTT AA^CGTT
AhdI 4915 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2661 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4915 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 4946 TGATCA T^GATCA
BseAI 6444 TCCGGA T^CCGGA
BspEI 6444 TCCGGA T^CCGGA
Bst1107I 5181 GTATAC GTA^TAC
BstZ17I 5181 GTATAC GTA^TAC
DrdI 160 GACAGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4708 CGGCCG C^GGCCG
Eam1105I 4915 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4708 CGGCCG C^GGCCG
Eco52I 4708 CGGCCG C^GGCCG
EcoT22I 106 ATGCAT ATGCA^T
FbaI 4946 TGATCA T^GATCA
HincII 472 GTTAAC GT[CT]^[GA]AC
HindII 472 GTTAAC GT[CT]^[GA]AC
HpaI 472 GTTAAC GTT^AAC
MroI 6444 TCCGGA T^CCGGA
NsiI 106 ATGCAT ATGCA^T
PshAI 400 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6027 AACGTT AA^CGTT
SgrAI 819 CGCCGGTG C[GA]^CCGG[CT]G
XmnI 2661 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 5917 GGTACC G^GTACC
6155 GGTACC
Asp718I 5917 GGTACC G^GTACC
6155 GGTACC
AspI 2020 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5930 GACAGGGTC
AviII 4415 TGCGCA TGC^GCA
4784 TGCGCA
BbrPI 2197 CACGTG CAC^GTG
6295 CACGTG
BsaBI 778 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2475 GATGAACATC
BsiEI 753 CGACCG CG[GA][CT]^CG
4708 CGGCCG
Bsp1407I 147 TGTACA T^GTACA
6657 TGTACA
BsrGI 147 TGTACA T^GTACA
6657 TGTACA
FspI 4415 TGCGCA TGC^GCA
4784 TGCGCA
KpnI 5917 GGTACC GGTAC^C
6155 GGTACC
MamI 778 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2475 GATGAACATC
MseI 473 TTAA T^TAA
5729 TTAA
PaeR7I 3415 CTCGAG C^TCGAG
3502 CTCGAG
PflFI 2020 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5930 GACAGGGTC
PmaCI 2197 CACGTG CAC^GTG
6295 CACGTG
PmlI 2197 CACGTG CAC^GTG
6295 CACGTG
SbfI 5610 CCTGCAGG CCTGCA^GG
6078 CCTGCAGG
ScaI 1394 AGTACT AGT^ACT
2318 AGTACT
Sse8387I 5610 CCTGCAGG CCTGCA^GG
6078 CCTGCAGG
SspBI 147 TGTACA T^GTACA
6657 TGTACA
Tru9I 473 TTAA T^TAA
5729 TTAA
Tth111I 2020 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5930 GACAGGGTC
XhoI 3415 CTCGAG C^TCGAG
3502 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NheI NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI