##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I AviII BbrPI BclI BfrI
BglII BsaBI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI EcoRV FbaI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SexAI
SgrAI SnaBI SpeI SphI SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 4316 ACACGT A^C[GA][CT]GT
AhdI 4585 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2568 ATTAAT AT^TAAT
AsnI 2568 ATTAAT AT^TAAT
AspEI 4585 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2333 CCTAGG C^CTAGG
BlnI 2333 CCTAGG C^CTAGG
BsaAI 2367 TACGTG [CT]AC^GT[GA]
BsmI 4408 GAATGC GAATGC(1/-1)
BspHI 3290 TCATGA T^CATGA
BstBI 4172 TTCGAA TT^CGAA
BstEII 581 GGTCACC G^GT[ACGT]ACC
BstPI 581 GGTCACC G^GT[ACGT]ACC
DraIII 2490 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2510 GACTCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4585 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 581 GGTCACC G^GT[ACGT]ACC
EcoT22I 2554 ATGCAT ATGCA^T
FseI 3678 GGCCGGCC GGCCGG^CC
NcoI 2260 CCATGG C^CATGG
NdeI 4604 CATATG CA^TATG
NsiI 2554 ATGCAT ATGCA^T
NspI 4377 GCATGT [GA]CATG^[CT]
NspV 4172 TTCGAA TT^CGAA
PaeR7I 5092 CTCGAG C^TCGAG
PshAI 2251 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2568 ATTAAT AT^TAAT
RcaI 3290 TCATGA T^CATGA
SalI 773 GTCGAC G^TCGAC
SbfI 3039 CCTGCAGG CCTGCA^GG
ScaI 5646 AGTACT AGT^ACT
SfiI 2418 GGCCCCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4172 TTCGAA TT^CGAA
Sse8387I 3039 CCTGCAGG CCTGCA^GG
XcmI 2699 CCAGCCGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5092 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 3125 GACGTC GACGT^C
4273 GACGTC
Alw44I 1464 GTGCAC G^TGCAC
5107 GTGCAC
AocI 358 CCTCAGG CC^T[ACGT]AGG
753 CCTAAGG
ApaLI 1464 GTGCAC G^TGCAC
5107 GTGCAC
AspI 3849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3986 GACTCAGTC
BalI 332 TGGCCA TGG^CCA
5774 TGGCCA
BciVI 2449 GGATAC GTATCC(6/5)
4863 GTATCC
BlpI 601 GCTCAGC GC^T[ACGT]AGC
3496 GCTGAGC
Bpu1102I 601 GCTCAGC GC^T[ACGT]AGC
3496 GCTGAGC
BssHII 271 GCGCGC G^CGCGC
273 GCGCGC
BssSI 3179 CTCGTG CACGAG(-5/-1)
5706 CTCGTG
Bsu36I 358 CCTCAGG CC^T[ACGT]AGG
753 CCTAAGG
CelII 601 GCTCAGC GC^T[ACGT]AGC
3496 GCTGAGC
Eco81I 358 CCTCAGG CC^T[ACGT]AGG
753 CCTAAGG
HincII 773 GTCGAC GT[CT]^[GA]AC
3817 GTTGAC
HindII 773 GTCGAC GT[CT]^[GA]AC
3817 GTTGAC
MluNI 332 TGGCCA TGG^CCA
5774 TGGCCA
MscI 332 TGGCCA TGG^CCA
5774 TGGCCA
NaeI 646 GCCGGC GCC^GGC
3679 GCCGGC
NgoMIV 646 GCCGGC G^CCGGC
3679 GCCGGC
PflFI 3849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3986 GACTCAGTC
PvuII 3206 CAGCTG CAG^CTG
5579 CAGCTG
SacI 3499 GAGCTC GAGCT^C
5872 GAGCTC
SspI 2009 AATATT AAT^ATT
2874 AATATT
StuI 4229 AGGCCT AGG^CCT
4385 AGGCCT
Tth111I 3849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3986 GACTCAGTC
XbaI 2631 TCTAGA T^CTAGA
2844 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI XmnI