##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AflII AgeI Aor51HI AscI AseI
AsnI BbrPI BfrI BsiWI BssHII
Bst1107I BstBI BstZ17I CpoI Eco47III
FseI KspI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacII SalI SbfI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AclI 5784 AACGTT AA^CGTT
AhdI 8979 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1050 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 8979 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2054 TGCGCA TGC^GCA
AvrII 6394 CCTAGG C^CTAGG
BlnI 6394 CCTAGG C^CTAGG
BsaAI 2392 TACGTG [CT]AC^GT[GA]
BsaBI 4057 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1855 ATCGAT AT^CGAT
BspLU11I 2462 ACATGT A^CATGT
ClaI 1855 ATCGAT AT^CGAT
EagI 10420 CGGCCG C^GGCCG
Eam1105I 8979 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 10420 CGGCCG C^GGCCG
Eco52I 10420 CGGCCG C^GGCCG
EcoRV 4540 GATATC GAT^ATC
FspI 2054 TGCGCA TGC^GCA
HpaI 5152 GTTAAC GTT^AAC
MamI 4057 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2483 CAATTG C^AATTG
MluI 1232 ACGCGT A^CGCGT
MunI 2483 CAATTG C^AATTG
NdeI 4181 CATATG CA^TATG
Psp1406I 5784 AACGTT AA^CGTT
ScaI 5703 AGTACT AGT^ACT
SfiI 1431 GGCCCCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 7902 AATATT AAT^ATT
XmnI 1050 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 825 GACGTC GACGT^C
1105 GACGTC
Acc65I 4610 GGTACC G^GTACC
6398 GGTACC
AccI 1108 GTCTAC GT^[AC][GT]AC
9579 GTAGAC
AccIII 2429 TCCGGA T^CCGGA
3008 TCCGGA
AflIII 1232 ACGCGT A^C[GA][CT]GT
2462 ACATGT
Asp718I 4610 GGTACC G^GTACC
6398 GGTACC
BcgI 2322 CGATGGTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7940 GCATGAGGATCG
BciVI 4911 GGATAC GTATCC(6/5)
5583 GGATAC
BsaWI 2429 TCCGGA [AT]^CCGG[AT]
3008 TCCGGA
BseAI 2429 TCCGGA T^CCGGA
3008 TCCGGA
BsiEI 4467 CGACCG CG[GA][CT]^CG
10420 CGGCCG
BsmI 9719 GAATGC GAATGC(1/-1)
10320 GAATGC
BspEI 2429 TCCGGA T^CCGGA
3008 TCCGGA
DrdI 6417 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
10345 GACCTCCTGGTC
EcoRI 2417 GAATTC G^AATTC
7813 GAATTC
EcoT22I 4178 ATGCAT ATGCA^T
6363 ATGCAT
KpnI 4610 GGTACC GGTAC^C
6398 GGTACC
MroI 2429 TCCGGA T^CCGGA
3008 TCCGGA
NsiI 4178 ATGCAT ATGCA^T
6363 ATGCAT
PflMI 3093 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3353 CCAGCCTCTGG
SexAI 2048 ACCAGGT A^CC[AT]GGT
3167 ACCTGGT
SpeI 2236 ACTAGT A^CTAGT
10402 ACTAGT
Van91I 3093 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3353 CCAGCCTCTGG
XbaI 7336 TCTAGA T^CTAGA
10745 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PpuMI PshAI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmaCI
XmaI