##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI BfrI BsiEI BsiWI
BspDI BssHII Bst1107I BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI KspI
MluI NdeI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SgrAI SmaI SnaBI SpeI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 526 GACGTC GACGT^C
Acc65I 2693 GGTACC G^GTACC
AccII 1044 CGCG CG^CG
AccIII 800 TCCGGA T^CCGGA
AhdI 5221 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2448 GTGCAC G^TGCAC
ApaLI 2448 GTGCAC G^TGCAC
Asp718I 2693 GGTACC G^GTACC
AspEI 5221 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4906 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1995 CCCGAG C^[CT]CG[GA]G
AviII 4385 TGCGCA TGC^GCA
AvrII 693 CCTAGG C^CTAGG
BbeI 3386 GGCGCC GGCGC^C
BbrPI 4018 CACGTG CAC^GTG
BbvCI 2611 GCTGAGG CCTCAGC(-5/-2)
BciVI 5454 GGATAC GTATCC(6/5)
BlnI 693 CCTAGG C^CTAGG
BsaAI 4018 CACGTG [CT]AC^GT[GA]
BseAI 800 TCCGGA T^CCGGA
BsmBI 5517 GAGACG CGTCTC(1/5)
BsoBI 1995 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1887 TGTACA T^GTACA
BspEI 800 TCCGGA T^CCGGA
BspLU11I 5554 ACATGT A^CATGT
BsrBI 2545 GAGCGG CCGCTC(-3/-3)
BsrGI 1887 TGTACA T^GTACA
BstBI 4475 TTCGAA TT^CGAA
BstUI 1044 CGCG CG^CG
Eam1105I 5221 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 4385 TGCGCA TGC^GCA
HaeII 3386 GGCGCC [GA]GCGC^[CT]
HpaI 3483 GTTAAC GTT^AAC
KasI 3386 GGCGCC G^GCGCC
KpnI 2693 GGTACC GGTAC^C
MfeI 3713 CAATTG C^AATTG
MroI 800 TCCGGA T^CCGGA
MunI 3713 CAATTG C^AATTG
MvnI 1044 CGCG CG^CG
NaeI 140 GCCGGC GCC^GGC
NarI 3386 GGCGCC GG^CGCC
NgoMIV 140 GCCGGC G^CCGGC
NheI 868 GCTAGC G^CTAGC
NspV 4475 TTCGAA TT^CGAA
PflFI 4906 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4018 CACGTG CAC^GTG
PmlI 4018 CACGTG CAC^GTG
SacI 2874 GAGCTC GAGCT^C
SexAI 3690 ACCTGGT A^CC[AT]GGT
SfiI 2289 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3386 GGCGCC GGC^GCC
SfuI 4475 TTCGAA TT^CGAA
SspBI 1887 TGTACA T^GTACA
Tth111I 4906 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 5132 CCACCTCACTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 27 GTCTAC GT^[AC][GT]AC
2560 GTAGAC
AcyI 526 GACGTC G[GA]^CG[CT]C
3386 GGCGCC
AflIII 4019 ACGTGT A^C[GA][CT]GT
5554 ACATGT
AocI 3251 CCTTAGG CC^T[ACGT]AGG
5223 CCTCAGG
ApaI 1680 GGGCCC GGGCC^C
4757 GGGCCC
BsaBI 2098 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5393 GATCATGATC
BsaHI 526 GACGTC G[GA]^CG[CT]C
3386 GGCGCC
BsaWI 800 TCCGGA [AT]^CCGG[AT]
5065 ACCGGA
BssSI 1709 CTCGTG CACGAG(-5/-1)
3094 CTCGTG
BstXI 2499 CCAGAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4958 CCAACATTCTGG
Bsu36I 3251 CCTTAGG CC^T[ACGT]AGG
5223 CCTCAGG
Eco81I 3251 CCTTAGG CC^T[ACGT]AGG
5223 CCTCAGG
EcoNI 3960 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4590 CCTCCTACAGG
EcoRI 2674 GAATTC G^AATTC
4632 GAATTC
EcoT22I 1172 ATGCAT ATGCA^T
2419 ATGCAT
HgaI 188 GCGTC GACGC(5/10)
2934 GCGTC
Hin1I 526 GACGTC G[GA]^CG[CT]C
3386 GGCGCC
HindIII 541 AAGCTT A^AGCTT
3149 AAGCTT
MamI 2098 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5393 GATCATGATC
NsiI 1172 ATGCAT ATGCA^T
2419 ATGCAT
SbfI 2055 CCTGCAGG CCTGCA^GG
4851 CCTGCAGG
ScaI 269 AGTACT AGT^ACT
1811 AGTACT
SphI 864 GCATGC GCATG^C
4721 GCATGC
Sse8387I 2055 CCTGCAGG CCTGCA^GG
4851 CCTGCAGG
SspI 665 AATATT AAT^ATT
1393 AATATT
StuI 650 AGGCCT AGG^CCT
2060 AGGCCT
XbaI 879 TCTAGA T^CTAGA
5365 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcgI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI