##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AvrII BbrPI BcgI BglI
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BstEII BstPI BstUI Bsu36I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FauI FseI KspI MamI
MluI MroI MvnI NaeI NgoMIV
NheI NotI NruI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 3405 GGTACC G^GTACC
AciI 3453 GCGG CCGC(-3/-1)
AcyI 3823 GGCGCC G[GA]^CG[CT]C
Asp700I 1170 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3405 GGTACC G^GTACC
AviII 3345 TGCGCA TGC^GCA
BalI 3706 TGGCCA TGG^CCA
BamHI 3813 GGATCC G^GATCC
BbeI 3823 GGCGCC GGCGC^C
BbvCI 3313 GCTGAGG CCTCAGC(-5/-2)
BciVI 4443 GTATCC GTATCC(6/5)
BclI 1130 TGATCA T^GATCA
BcnI 439 CCGGG CC^[GC]GG
BlpI 2947 GCTGAGC GC^T[ACGT]AGC
BpmI 5256 CTGGAG CTGGAG(16/14)
Bpu1102I 2947 GCTGAGC GC^T[ACGT]AGC
BsaAI 3152 TACGTG [CT]AC^GT[GA]
BsaHI 3823 GGCGCC G[GA]^CG[CT]C
BsmAI 382 GAGAC GTCTC(1/5)
BssSI 2372 CTCGTG CACGAG(-5/-1)
BstAPI 1594 GCAATACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4022 TTCGAA TT^CGAA
CelII 2947 GCTGAGC GC^T[ACGT]AGC
DraII 2458 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 4754 GACATATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3706 TGGCCA [CT]^GGCC[GA]
EcoO109I 2458 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 3591 GATATC GAT^ATC
FbaI 1130 TGATCA T^GATCA
FspI 3345 TGCGCA TGC^GCA
HaeII 3823 GGCGCC [GA]GCGC^[CT]
HapII 439 CCGG C^CGG
HgaI 2983 GCGTC GACGC(5/10)
Hin1I 3823 GGCGCC G[GA]^CG[CT]C
HindIII 2735 AAGCTT A^AGCTT
HpaII 439 CCGG C^CGG
KasI 3823 GGCGCC G^GCGCC
KpnI 3405 GGTACC GGTAC^C
MluNI 3706 TGGCCA TGG^CCA
MscI 3706 TGGCCA TGG^CCA
MspA1I 3323 CAGCTG C[AC]G^C[GT]G
MspI 439 CCGG C^CGG
NarI 3823 GGCGCC GG^CGCC
NciI 439 CCGGG CC^[GC]GG
NspV 4022 TTCGAA TT^CGAA
PacI 304 TTAATTAA TTAAT^TAA
PpuMI 2458 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3832 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 495 CTGCAG CTGCA^G
PvuII 3323 CAGCTG CAG^CTG
ScaI 4507 AGTACT AGT^ACT
SfoI 3823 GGCGCC GGC^GCC
SfuI 4022 TTCGAA TT^CGAA
XbaI 4208 TCTAGA T^CTAGA
XcmI 4574 CCAAGTTGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1170 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 2792 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
3320 CAGCAGCTG
BanI 3405 GGTACC G^G[CT][GA]CC
3823 GGCGCC
BglII 1428 AGATCT A^GATCT
2366 AGATCT
BseRI 3306 GAGGAG GAGGAG(10/8)
3729 GAGGAG
BsmFI 2404 GTCCC GGGAC(10/14)
4216 GTCCC
DsaI 1357 CCATGG C^C[GA][CT]GG
3390 CCATGG
EcoT22I 1800 ATGCAT ATGCA^T
5055 ATGCAT
HpaI 171 GTTAAC GTT^AAC
2912 GTTAAC
MfeI 750 CAATTG C^AATTG
3975 CAATTG
MunI 750 CAATTG C^AATTG
3975 CAATTG
NcoI 1357 CCATGG C^CATGG
3390 CCATGG
NsiI 1800 ATGCAT ATGCA^T
5055 ATGCAT
PleI 2595 GAGTC GAGTC(4/5)
4214 GAGTC
SapI 3071 GAAGAGC GCTCTTC(1/4)
103 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanII BbsI BbvI BfaI
BfrI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI Bst1107I BstF5I BstNI
BstXI BstYI BstZ17I Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PshBI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII