##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AscI AviII BbeI
BbrPI BcgI BfrI BglI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BstBI
BstUI CelII Cfr10I ClaI CpoI
DsaI EagI EclXI Eco52I EcoRI
FauI FseI FspI Hin1I HpaI
KasI KspI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PaeR7I PinAI PmaCI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2540 GGTACC G^GTACC
AhdI 1025 GACACAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1659 GTGCAC G^TGCAC
Aor51HI 3398 AGCGCT AGC^GCT
ApaLI 1659 GTGCAC G^TGCAC
Asp718I 2540 GGTACC G^GTACC
AspEI 1025 GACACAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4080 CCTAGG C^CTAGG
BalI 560 TGGCCA TGG^CCA
BlnI 4080 CCTAGG C^CTAGG
BsmI 2004 GAATGC GAATGC(1/-1)
BspHI 1907 TCATGA T^CATGA
BssSI 784 CTCGTG CACGAG(-5/-1)
Bst1107I 1277 GTATAC GTA^TAC
BstAPI 1622 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 587 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1277 GTATAC GTA^TAC
DraIII 2391 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1526 GACACTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 560 TGGCCA [CT]^GGCC[GA]
Eam1105I 1025 GACACAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3398 AGCGCT AGC^GCT
EcoNI 5012 CCTTAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2519 GATATC GAT^ATC
EcoT22I 1771 ATGCAT ATGCA^T
HaeII 3398 AGCGCT [GA]GCGC^[CT]
HgaI 1031 GCGTC GACGC(5/10)
KpnI 2540 GGTACC GGTAC^C
MfeI 5768 CAATTG C^AATTG
MluNI 560 TGGCCA TGG^CCA
MscI 560 TGGCCA TGG^CCA
MspA1I 1545 CAGCTG C[AC]G^C[GT]G
MunI 5768 CAATTG C^AATTG
NsiI 1771 ATGCAT ATGCA^T
PacI 3027 TTAATTAA TTAAT^TAA
PmeI 4613 GTTTAAAC GTTT^AAAC
PstI 2140 CTGCAG CTGCA^G
PvuII 1545 CAGCTG CAG^CTG
RcaI 1907 TCATGA T^CATGA
SapI 280 GCTCTTC GCTCTTC(1/4)
ScaI 3250 AGTACT AGT^ACT
SmlI 2335 CTCAAG C^T[CT][GA]AG
SwaI 5668 ATTTAAAT ATTT^AAAT
XcmI 4163 CCAAAAGGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 1311 CCTCAGG CC^T[ACGT]AGG
2230 CCTCAGG
ApaI 518 GGGCCC GGGCC^C
542 GGGCCC
AspHI 78 GAGCAC G[AT]GC[AT]^C
1659 GTGCAC
AvaI 515 CCCGGG C^[CT]CG[GA]G
539 CCCGGG
BamHI 59 GGATCC G^GATCC
879 GGATCC
BanI 857 GGTGCC G^G[CT][GA]CC
2540 GGTACC
BbvCI 1537 CCTCAGC CCTCAGC(-5/-2)
4204 GCTGAGG
BciVI 891 GGATAC GTATCC(6/5)
1669 GTATCC
BclI 377 TGATCA T^GATCA
5323 TGATCA
BglII 990 AGATCT A^GATCT
2104 AGATCT
BsaBI 1068 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3005 GATTATAATC
BsiHKAI 78 GAGCAC G[AT]GC[AT]^C
1659 GTGCAC
BsoBI 515 CCCGGG C^[CT]CG[GA]G
539 CCCGGG
Bsu36I 1311 CCTCAGG CC^T[ACGT]AGG
2230 CCTCAGG
CfoI 44 GCGC GCG^C
3399 GCGC
Eco81I 1311 CCTCAGG CC^T[ACGT]AGG
2230 CCTCAGG
FbaI 377 TGATCA T^GATCA
5323 TGATCA
HhaI 44 GCGC GCG^C
3399 GCGC
HinP1I 44 GCGC G^CGC
3399 GCGC
HincII 5345 GTTGAC GT[CT]^[GA]AC
5729 GTTGAC
HindII 5345 GTTGAC GT[CT]^[GA]AC
5729 GTTGAC
MaeII 4301 ACGT A^CGT
4619 ACGT
MamI 1068 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3005 GATTATAATC
NheI 2681 GCTAGC G^CTAGC
3310 GCTAGC
PpuMI 554 AGGTCCT [GA]G^G[AT]CC[CT]
5504 GGGTCCT
PshAI 363 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2403 GACCATCGTC
SmaI 515 CCCGGG CCC^GGG
539 CCCGGG
XmaCI 515 CCCGGG C^CCGGG
539 CCCGGG
XmaI 515 CCCGGG C^CCGGG
539 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SpeI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoII XmnI