##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Aor51HI ApaI AscI
Asp718I AviII BamHI BbeI BbrPI
BcgI BlpI Bpu1102I BsaHI BseAI
BsiEI BsiWI BspDI BspEI BssHII
BstBI BstUI CelII ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoNI FseI FspI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NarI NdeI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SbfI SfiI
SfoI SfuI SgrAI SpeI Sse8387I
StuI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AflIII 5182 ACATGT A^C[GA][CT]GT
AhdI 2430 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3897 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1576 CCTCAGG CC^T[ACGT]AGG
Asp700I 4678 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2430 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1355 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1899 CCTAGG C^CTAGG
BanII 5173 GAGCCC G[GA]GC[CT]^C
BclI 2317 TGATCA T^GATCA
BglI 884 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4054 AGATCT A^GATCT
BlnI 1899 CCTAGG C^CTAGG
BsaAI 1695 TACGTA [CT]AC^GT[GA]
BsaBI 1766 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3829 ACCGGA [AT]^CCGG[AT]
BsmI 3853 GAATGC GAATGC(1/-1)
Bsp1407I 778 TGTACA T^GTACA
BspLU11I 5182 ACATGT A^CATGT
BsrBI 4895 GAGCGG CCGCTC(-3/-3)
BsrDI 287 GCAATG GCAATG(2/0)
BsrFI 889 GCCGGC [GA]^CCGG[CT]
BsrGI 778 TGTACA T^GTACA
BssSI 3600 CTCGTG CACGAG(-5/-1)
Bst1107I 1257 GTATAC GTA^TAC
BstAPI 4730 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4217 GGTGACC G^GT[ACGT]ACC
BstPI 4217 GGTGACC G^GT[ACGT]ACC
BstZ17I 1257 GTATAC GTA^TAC
Bsu36I 1576 CCTCAGG CC^T[ACGT]AGG
Cfr10I 889 GCCGGC [GA]^CCGG[CT]
DrdI 2335 GACAGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2430 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1576 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4217 GGTGACC G^GT[ACGT]ACC
EcoRV 2448 GATATC GAT^ATC
FbaI 2317 TGATCA T^GATCA
HincII 5155 GTCGAC GT[CT]^[GA]AC
HindII 5155 GTCGAC GT[CT]^[GA]AC
MamI 1766 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 889 GCCGGC GCC^GGC
NgoMIV 889 GCCGGC G^CCGGC
NheI 750 GCTAGC G^CTAGC
PaeR7I 4892 CTCGAG C^TCGAG
PflFI 1355 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 53 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2627 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 4953 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 5155 GTCGAC G^TCGAC
SexAI 903 ACCTGGT A^CC[AT]GGT
SmaI 1425 CCCGGG CCC^GGG
SnaBI 1695 TACGTA TAC^GTA
SphI 3102 GCATGC GCATG^C
SspBI 778 TGTACA T^GTACA
SspI 2918 AATATT AAT^ATT
Tth111I 1355 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 53 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2554 TCTAGA T^CTAGA
XhoI 4892 CTCGAG C^TCGAG
XmaCI 1425 CCCGGG C^CCGGG
XmaI 1425 CCCGGG C^CCGGG
XmnI 4678 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1480 GTGCAC G^TGCAC
2488 GTGCAC
ApaLI 1480 GTGCAC G^TGCAC
2488 GTGCAC
AseI 2506 ATTAAT AT^TAAT
2875 ATTAAT
AsnI 2506 ATTAAT AT^TAAT
2875 ATTAAT
AspHI 1480 GTGCAC G[AT]GC[AT]^C
2488 GTGCAC
AvaII 2628 GGTCC G^G[AT]CC
4987 GGTCC
BsiHKAI 1480 GTGCAC G[AT]GC[AT]^C
2488 GTGCAC
BsmBI 159 GAGACG CGTCTC(1/5)
1530 GAGACG
BstXI 827 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1604 CCAAAGTGCTGG
DsaI 3285 CCATGG C^C[GA][CT]GG
5016 CCATGG
EcoRI 369 GAATTC G^AATTC
4131 GAATTC
EcoT22I 711 ATGCAT ATGCA^T
3100 ATGCAT
FauI 3301 GCGGG CCCGC(4/6)
4897 GCGGG
HaeII 3278 AGCGCC [GA]GCGC^[CT]
4558 GGCGCT
HgaI 2742 GACGC GACGC(5/10)
3728 GACGC
HindIII 1414 AAGCTT A^AGCTT
2650 AAGCTT
NcoI 3285 CCATGG C^CATGG
5016 CCATGG
NsiI 711 ATGCAT ATGCA^T
3100 ATGCAT
PshBI 2506 ATTAAT AT^TAAT
2875 ATTAAT
SapI 3344 GAAGAGC GCTCTTC(1/4)
3905 GAAGAGC
ScaI 350 AGTACT AGT^ACT
3713 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AvaI BalI
BanI BbsI BbvCI BbvI BciVI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI SwaI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII