##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BglI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV FauI
FseI FspI HgaI Hin1I KasI
KpnI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NgoMIV
NheI NotI NruI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI Sse8387I
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AclI 1887 AACGTT AA^CGTT
AvaI 6095 CTCGAG C^[CT]CG[GA]G
BciVI 2187 GGATAC GTATCC(6/5)
BcnI 5799 CCGGG CC^[GC]GG
BsaAI 5047 TACGTA [CT]AC^GT[GA]
BsmI 3516 GAATGC GAATGC(1/-1)
BsoBI 6095 CTCGAG C^[CT]CG[GA]G
BspMI 5939 GCAGGT ACCTGC(4/8)
BsrFI 4188 GCCGGT [GA]^CCGG[CT]
BstXI 5081 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 4188 GCCGGT [GA]^CCGG[CT]
DraIII 2909 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1868 CCATGG C^C[GA][CT]GG
EcoNI 6744 CCTAGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HpaI 3982 GTTAAC GTT^AAC
NciI 5799 CCGGG CC^[GC]GG
NcoI 1868 CCATGG C^CATGG
NdeI 1811 CATATG CA^TATG
PacI 3235 TTAATTAA TTAAT^TAA
PaeR7I 6095 CTCGAG C^TCGAG
PpuMI 1655 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1887 AACGTT AA^CGTT
PstI 6152 CTGCAG CTGCA^G
SacI 4845 GAGCTC GAGCT^C
SnaBI 5047 TACGTA TAC^GTA
SpeI 5968 ACTAGT A^CTAGT
StuI 5347 AGGCCT AGG^CCT
XhoI 6095 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1003 GTAGAC GT^[AC][GT]AC
4233 GTCTAC
Aor51HI 127 AGCGCT AGC^GCT
3752 AGCGCT
AseI 2107 ATTAAT AT^TAAT
3183 ATTAAT
AsnI 2107 ATTAAT AT^TAAT
3183 ATTAAT
Asp700I 5029 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5507 GAAACATTTC
BanII 2014 GAGCCC G[GA]GC[CT]^C
4845 GAGCTC
BseRI 59 CTCCTC GAGGAG(10/8)
6178 CTCCTC
BsgI 663 CTGCAC GTGCAG(16/14)
5999 GTGCAG
BspHI 2505 TCATGA T^CATGA
4833 TCATGA
BsrDI 4184 CATTGC GCAATG(2/0)
1615 GCAATG
BstAPI 4283 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5710 GCAGCACTTGC
BstBI 437 TTCGAA TT^CGAA
906 TTCGAA
BstEII 6112 GGTGACC G^GT[ACGT]ACC
6318 GGTTACC
BstPI 6112 GGTGACC G^GT[ACGT]ACC
6318 GGTTACC
CfoI 128 GCGC GCG^C
3753 GCGC
DraII 1655 AGGACCT [GA]G^G[ACGT]CC[CT]
5645 AGGCCCC
Eco47III 127 AGCGCT AGC^GCT
3752 AGCGCT
EcoO109I 1655 AGGACCT [GA]G^G[ACGT]CC[CT]
5645 AGGCCCC
EcoO65I 6112 GGTGACC G^GT[ACGT]ACC
6318 GGTTACC
HaeII 127 AGCGCT [GA]GCGC^[CT]
3752 AGCGCT
HapII 4189 CCGG C^CGG
5799 CCGG
HhaI 128 GCGC GCG^C
3753 GCGC
HinP1I 128 GCGC G^CGC
3753 GCGC
HpaII 4189 CCGG C^CGG
5799 CCGG
MfeI 2300 CAATTG C^AATTG
6576 CAATTG
MspI 4189 CCGG C^CGG
5799 CCGG
MunI 2300 CAATTG C^AATTG
6576 CAATTG
NspV 437 TTCGAA TT^CGAA
906 TTCGAA
PflMI 2469 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2908 CCACGACGTGG
PshBI 2107 ATTAAT AT^TAAT
3183 ATTAAT
RcaI 2505 TCATGA T^CATGA
4833 TCATGA
SfuI 437 TTCGAA TT^CGAA
906 TTCGAA
SphI 3837 GCATGC GCATG^C
4587 GCATGC
SwaI 5150 ATTTAAAT ATTT^AAAT
6641 ATTTAAAT
Van91I 2469 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2908 CCACGACGTGG
XmnI 5029 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5507 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BclI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII