##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Aor51HI ApaI AscI
Asp718I AviII AvrII BamHI BanI
BbeI BbrPI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BsiWI BsmBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII Cfr10I CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoT22I FseI FspI
HgaI Hin1I HindIII KasI KpnI
KspI MluI MvnI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 3528 TCCGGA T^CCGGA
AflIII 2894 ACATGT A^C[GA][CT]GT
AhdI 460 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2898 GTGCAC G^TGCAC
ApaLI 2898 GTGCAC G^TGCAC
Asp700I 4348 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 460 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3009 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3362 CGATGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4931 GTATCC GTATCC(6/5)
BclI 4359 TGATCA T^GATCA
BglI 204 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3528 TCCGGA [AT]^CCGG[AT]
BseAI 3528 TCCGGA T^CCGGA
BsiEI 4448 CGACCG CG[GA][CT]^CG
BsmI 372 GCATTC GAATGC(1/-1)
BspDI 3360 ATCGAT AT^CGAT
BspEI 3528 TCCGGA T^CCGGA
BspHI 4356 TCATGA T^CATGA
BspLU11I 2894 ACATGT A^CATGT
BspMI 4601 ACCTGC ACCTGC(4/8)
BstAPI 5284 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5166 GGTGACC G^GT[ACGT]ACC
BstPI 5166 GGTGACC G^GT[ACGT]ACC
ClaI 3360 ATCGAT AT^CGAT
DraIII 4164 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 460 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5166 GGTGACC G^GT[ACGT]ACC
FbaI 4359 TGATCA T^GATCA
HpaI 1644 GTTAAC GTT^AAC
MfeI 1825 CAATTG C^AATTG
MroI 3528 TCCGGA T^CCGGA
MunI 1825 CAATTG C^AATTG
NheI 831 GCTAGC G^CTAGC
PflFI 3009 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4911 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 4356 TCATGA T^CATGA
SapI 3703 GAAGAGC GCTCTTC(1/4)
SexAI 4060 ACCAGGT A^CC[AT]GGT
SmaI 3719 CCCGGG CCC^GGG
SpeI 2946 ACTAGT A^CTAGT
Tth111I 3009 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1481 CCACACCGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3719 CCCGGG C^CCGGG
XmaI 3719 CCCGGG C^CCGGG
XmnI 4348 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 34 GAATTC [GA]^AATT[CT]
2484 GAATTC
ApoI 34 GAATTC [GA]^AATT[CT]
2484 GAATTC
AseI 825 ATTAAT AT^TAAT
3102 ATTAAT
AsnI 825 ATTAAT AT^TAAT
3102 ATTAAT
AvaI 148 CCCGAG C^[CT]CG[GA]G
3719 CCCGGG
BbvCI 10 CCTCAGC CCTCAGC(-5/-2)
2996 CCTCAGC
BsoBI 148 CCCGAG C^[CT]CG[GA]G
3719 CCCGGG
BsrBI 1135 CCGCTC CCGCTC(-3/-3)
4530 CCGCTC
CfoI 53 GCGC GCG^C
3295 GCGC
EcoRI 34 GAATTC G^AATTC
2484 GAATTC
HaeII 52 AGCGCC [GA]GCGC^[CT]
3294 AGCGCC
HhaI 53 GCGC GCG^C
3295 GCGC
HinP1I 53 GCGC G^CGC
3295 GCGC
MaeII 359 ACGT A^CGT
3208 ACGT
PshAI 3047 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4028 GACATTTGTC
PshBI 825 ATTAAT AT^TAAT
3102 ATTAAT
PstI 728 CTGCAG CTGCA^G
1934 CTGCAG
PvuII 1067 CAGCTG CAG^CTG
4174 CAGCTG
TaqI 3361 TCGA T^CGA
4447 TCGA
TthHB8I 3361 TCGA T^CGA
4447 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XbaI XhoII