##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
SspBI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 2657 GTGCAC G^TGCAC
AocI 319 CCTGAGG CC^T[ACGT]AGG
ApaLI 2657 GTGCAC G^TGCAC
AvaI 1181 CCCGAG C^[CT]CG[GA]G
BanI 3184 GGTGCC G^G[CT][GA]CC
BbsI 3485 GAAGAC GAAGAC(2/6)
BbvCI 1172 CCTCAGC CCTCAGC(-5/-2)
BcgI 3087 CGAAAAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2429 CCGGG CC^[GC]GG
BglII 2961 AGATCT A^GATCT
BpuAI 3485 GAAGAC GAAGAC(2/6)
BsaAI 4232 TACGTA [CT]AC^GT[GA]
BsaI 4428 GGTCTC GGTCTC(1/5)
BsoBI 1181 CCCGAG C^[CT]CG[GA]G
BspMI 766 GCAGGT ACCTGC(4/8)
BsrFI 2373 ACCGGC [GA]^CCGG[CT]
BstXI 1323 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 319 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2373 ACCGGC [GA]^CCGG[CT]
Eco81I 319 CCTGAGG CC^T[ACGT]AGG
EcoRI 1285 GAATTC G^AATTC
EcoRV 2681 GATATC GAT^ATC
NciI 2429 CCGGG CC^[GC]GG
SacI 4522 GAGCTC GAGCT^C
SapI 107 GAAGAGC GCTCTTC(1/4)
ScaI 214 AGTACT AGT^ACT
SnaBI 4232 TACGTA TAC^GTA
StuI 316 AGGCCT AGG^CCT
SwaI 4566 ATTTAAAT ATTT^AAAT
XbaI 3133 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 511 GCGG CCGC(-3/-1)
3236 GCGG
Asp700I 439 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2433 GAATGACTTC
AvaII 2258 GGACC G^G[AT]CC
3544 GGTCC
BalI 1269 TGGCCA TGG^CCA
4400 TGGCCA
BanII 110 GAGCCC G[GA]GC[CT]^C
4522 GAGCTC
BsgI 1110 GTGCAG GTGCAG(16/14)
2337 GTGCAG
BsmI 592 GCATTC GAATGC(1/-1)
2629 GCATTC
BspLU11I 2403 ACATGT A^CATGT
4910 ACATGT
BstAPI 1136 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2741 GCAAATTGTGC
BstBI 148 TTCGAA TT^CGAA
3264 TTCGAA
Cfr13I 2258 GGACC G^G[ACGT]CC
3544 GGTCC
DraII 2257 AGGACCT [GA]G^G[ACGT]CC[CT]
3543 GGGTCCC
DraIII 574 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3549 CACATGGTG
DsaI 506 CCATGG C^C[GA][CT]GG
2782 CCATGG
EaeI 1269 TGGCCA [CT]^GGCC[GA]
4400 TGGCCA
EcoO109I 2257 AGGACCT [GA]G^G[ACGT]CC[CT]
3543 GGGTCCC
HindIII 2394 AAGCTT A^AGCTT
4360 AAGCTT
HpaI 1965 GTTAAC GTT^AAC
4995 GTTAAC
MluNI 1269 TGGCCA TGG^CCA
4400 TGGCCA
MscI 1269 TGGCCA TGG^CCA
4400 TGGCCA
MwoI 1136 GCAACCTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2741 GCAAATTGTGC
NcoI 506 CCATGG C^CATGG
2782 CCATGG
NspV 148 TTCGAA TT^CGAA
3264 TTCGAA
PpuMI 2257 AGGACCT [GA]G^G[AT]CC[CT]
3543 GGGTCCC
Sau96I 2258 GGACC G^G[ACGT]CC
3544 GGTCC
SfuI 148 TTCGAA TT^CGAA
3264 TTCGAA
SphI 939 GCATGC GCATG^C
3384 GCATGC
XcmI 2875 CCAGTCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3455 CCAGTTATTCAGTGG
XmnI 439 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2433 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AseI AsnI AspHI
BbvI BclI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PshBI
PvuII RsaI Sau3AI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII