##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII BalI BanI
BbeI BbrPI BcgI BciVI BclI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspLU11I BsrFI BssHII
Bst1107I BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MaeII
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Aor51HI 1271 AGCGCT AGC^GCT
AseI 2457 ATTAAT AT^TAAT
AsnI 2457 ATTAAT AT^TAAT
Asp700I 1707 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4922 CCTAGG C^CTAGG
BamHI 3666 GGATCC G^GATCC
BanII 1599 GAGCCC G[GA]GC[CT]^C
BbsI 3645 GTCTTC GAAGAC(2/6)
BlnI 4922 CCTAGG C^CTAGG
BpuAI 3645 GTCTTC GAAGAC(2/6)
BsaI 801 GAGACC GGTCTC(1/5)
BsaWI 2395 TCCGGT [AT]^CCGG[AT]
BsmBI 4490 CGTCTC CGTCTC(1/5)
Bsp1407I 4175 TGTACA T^GTACA
BspHI 3798 TCATGA T^CATGA
BsrBI 704 GAGCGG CCGCTC(-3/-3)
BsrDI 3424 CATTGC GCAATG(2/0)
BsrGI 4175 TGTACA T^GTACA
BssSI 1055 CTCGTG CACGAG(-5/-1)
BstBI 884 TTCGAA TT^CGAA
BstEII 1854 GGTGACC G^GT[ACGT]ACC
BstPI 1854 GGTGACC G^GT[ACGT]ACC
DraIII 1696 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2125 CCATGG C^C[GA][CT]GG
EaeI 74 CGGCCA [CT]^GGCC[GA]
Eco47III 1271 AGCGCT AGC^GCT
EcoNI 31 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1854 GGTGACC G^GT[ACGT]ACC
EcoRV 1560 GATATC GAT^ATC
HaeII 1271 AGCGCT [GA]GCGC^[CT]
MslI 1848 CATATAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2125 CCATGG C^CATGG
NspV 884 TTCGAA TT^CGAA
PacI 2660 TTAATTAA TTAAT^TAA
PshBI 2457 ATTAAT AT^TAAT
RcaI 3798 TCATGA T^CATGA
SapI 3396 GAAGAGC GCTCTTC(1/4)
SexAI 980 ACCTGGT A^CC[AT]GGT
SfuI 884 TTCGAA TT^CGAA
SspBI 4175 TGTACA T^GTACA
SspI 2983 AATATT AAT^ATT
XmnI 1707 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 450 GTAGAC GT^[AC][GT]AC
3659 GTAGAC
AflII 2347 CTTAAG C^TTAAG
5049 CTTAAG
BcnI 72 CCCGG CC^[GC]GG
1219 CCGGG
BfrI 2347 CTTAAG C^TTAAG
5049 CTTAAG
BglII 2227 AGATCT A^GATCT
2553 AGATCT
BseRI 2632 CTCCTC GAGGAG(10/8)
63 GAGGAG
CfoI 740 GCGC GCG^C
1272 GCGC
FauI 24 CCCGC CCCGC(4/6)
2812 GCGGG
HhaI 740 GCGC GCG^C
1272 GCGC
HinP1I 740 GCGC G^CGC
1272 GCGC
HincII 2873 GTTAAC GT[CT]^[GA]AC
4843 GTTAAC
HindII 2873 GTTAAC GT[CT]^[GA]AC
4843 GTTAAC
HpaI 2873 GTTAAC GTT^AAC
4843 GTTAAC
NciI 72 CCCGG CC^[GC]GG
1219 CCGGG
PshAI 803 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1425 GACATGAGTC
PvuII 1033 CAGCTG CAG^CTG
1444 CAGCTG
SpeI 1770 ACTAGT A^CTAGT
1812 ACTAGT
SphI 1025 GCATGC GCATG^C
3458 GCATGC
StuI 1994 AGGCCT AGG^CCT
4679 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BbvCI BbvI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII