##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BbeI BbrPI BlpI Bpu1102I
BsaBI BsaHI BsiWI Bsp1407I BspDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2891 GTAGAC GT^[AC][GT]AC
AhdI 3790 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3574 GGGCCC GGGCC^C
AspEI 3790 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2556 CTCGGG C^[CT]CG[GA]G
AvrII 2751 CCTAGG C^CTAGG
BcgI 1862 CGATTTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 94 TGATCA T^GATCA
BglI 2587 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2751 CCTAGG C^CTAGG
BsiEI 33 CGACCG CG[GA][CT]^CG
BsmI 1567 GAATGC GAATGC(1/-1)
BsoBI 2556 CTCGGG C^[CT]CG[GA]G
BspLU11I 4789 ACATGT A^CATGT
BsrBI 1298 CCGCTC CCGCTC(-3/-3)
BssSI 367 CACGAG CACGAG(-5/-1)
BstAPI 1806 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 1244 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 3790 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1244 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 280 GAATTC G^AATTC
FbaI 94 TGATCA T^GATCA
HgaI 1179 GCGTC GACGC(5/10)
NdeI 4492 CATATG CA^TATG
PpuMI 1244 GGGACCT [GA]G^G[AT]CC[CT]
ScaI 1548 AGTACT AGT^ACT
SexAI 3183 ACCAGGT A^CC[AT]GGT
SpeI 4105 ACTAGT A^CTAGT
SspI 2112 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 891 TCCGGA T^CCGGA
4469 TCCGGA
Alw44I 868 GTGCAC G^TGCAC
2856 GTGCAC
ApaLI 868 GTGCAC G^TGCAC
2856 GTGCAC
Asp700I 808 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3086 GAATAAGTTC
BamHI 1350 GGATCC G^GATCC
2280 GGATCC
BglII 441 AGATCT A^GATCT
1939 AGATCT
BseAI 891 TCCGGA T^CCGGA
4469 TCCGGA
BsmBI 2493 CGTCTC CGTCTC(1/5)
305 GAGACG
BspEI 891 TCCGGA T^CCGGA
4469 TCCGGA
BsrDI 3038 CATTGC GCAATG(2/0)
4151 GCAATG
EarI 1904 CTCTTC CTCTTC(1/4)
2212 CTCTTC
HindIII 787 AAGCTT A^AGCTT
3432 AAGCTT
Ksp632I 1904 CTCTTC CTCTTC(1/4)
2212 CTCTTC
MfeI 3760 CAATTG C^AATTG
4014 CAATTG
MroI 891 TCCGGA T^CCGGA
4469 TCCGGA
MslI 19 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
295 CACTCCTGTG
MunI 3760 CAATTG C^AATTG
4014 CAATTG
SphI 2192 GCATGC GCATG^C
4945 GCATGC
StuI 636 AGGCCT AGG^CCT
2347 AGGCCT
XmnI 808 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3086 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AspI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI Eco81I EcoNI
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII