##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AlwNI AocI Aor51HI ApaI AscI
AviII BamHI BbeI BbrPI BcgI
BclI BglI BsaBI BsaWI BseAI
BsiEI BspDI BspEI BsrFI BssHII
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
FbaI FseI FspI HaeII HpaI
KasI KspI MamI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 424 GGTACC G^GTACC
AcyI 3533 GACGCC G[GA]^CG[CT]C
AhdI 2036 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1444 GTGCAC G^TGCAC
ApaLI 1444 GTGCAC G^TGCAC
AseI 3045 ATTAAT AT^TAAT
AsnI 3045 ATTAAT AT^TAAT
Asp700I 2657 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 424 GGTACC G^GTACC
AspEI 2036 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3359 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3471 TGGCCA TGG^CCA
BanI 424 GGTACC G^G[CT][GA]CC
BciVI 1487 GGATAC GTATCC(6/5)
BglII 2584 AGATCT A^GATCT
BlpI 2456 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2456 GCTAAGC GC^T[ACGT]AGC
BsaAI 4483 TACGTA [CT]AC^GT[GA]
BsaHI 3533 GACGCC G[GA]^CG[CT]C
BsiWI 2003 CGTACG C^GTACG
Bsp1407I 2871 TGTACA T^GTACA
BspHI 3443 TCATGA T^CATGA
BspMI 4462 GCAGGT ACCTGC(4/8)
BsrBI 4784 CCGCTC CCGCTC(-3/-3)
BsrGI 2871 TGTACA T^GTACA
BssSI 1557 CTCGTG CACGAG(-5/-1)
BstAPI 3593 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 229 GGTGACC G^GT[ACGT]ACC
BstPI 229 GGTGACC G^GT[ACGT]ACC
CelII 2456 GCTAAGC GC^T[ACGT]AGC
DraII 465 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 2036 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 465 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 229 GGTGACC G^GT[ACGT]ACC
EcoRI 1998 GAATTC G^AATTC
EcoT22I 2860 ATGCAT ATGCA^T
Hin1I 3533 GACGCC G[GA]^CG[CT]C
KpnI 424 GGTACC GGTAC^C
MluNI 3471 TGGCCA TGG^CCA
MscI 3471 TGGCCA TGG^CCA
MspA1I 1213 CAGCTG C[AC]G^C[GT]G
NsiI 2860 ATGCAT ATGCA^T
PaeR7I 3960 CTCGAG C^TCGAG
PflFI 3359 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 470 CCACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 465 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 3045 ATTAAT AT^TAAT
PvuII 1213 CAGCTG CAG^CTG
RcaI 3443 TCATGA T^CATGA
SacI 439 GAGCTC GAGCT^C
ScaI 4660 AGTACT AGT^ACT
SnaBI 4483 TACGTA TAC^GTA
SpeI 3279 ACTAGT A^CTAGT
SspBI 2871 TGTACA T^GTACA
SwaI 2321 ATTTAAAT ATTT^AAAT
Tth111I 3359 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 470 CCACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1133 TCTAGA T^CTAGA
XhoI 3960 CTCGAG C^TCGAG
XmnI 2657 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1530 CGCG CG^CG
3617 CGCG
AflII 1511 CTTAAG C^TTAAG
4390 CTTAAG
BfrI 1511 CTTAAG C^TTAAG
4390 CTTAAG
BsgI 431 GTGCAG GTGCAG(16/14)
2414 GTGCAG
BsmBI 3491 CGTCTC CGTCTC(1/5)
3357 GAGACG
BspLU11I 1980 ACATGT A^CATGT
2903 ACATGT
BstUI 1530 CGCG CG^CG
3617 CGCG
BstXI 121 CCATAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3413 CCAGCACTTTGG
Cac8I 3434 GCGGGC GC[ACGT]^[ACGT]GC
3469 GCTGGC
EaeI 2093 TGGCCG [CT]^GGCC[GA]
3471 TGGCCA
EcoRV 3188 GATATC GAT^ATC
4056 GATATC
FauI 1806 CCCGC CCCGC(4/6)
3434 GCGGG
HgaI 1137 GACGC GACGC(5/10)
3533 GACGC
HincII 818 GTTGAC GT[CT]^[GA]AC
1009 GTCAAC
HindII 818 GTTGAC GT[CT]^[GA]AC
1009 GTCAAC
HindIII 93 AAGCTT A^AGCTT
1789 AAGCTT
MfeI 165 CAATTG C^AATTG
2645 CAATTG
MunI 165 CAATTG C^AATTG
2645 CAATTG
MvnI 1530 CGCG CG^CG
3617 CGCG
NdeI 2615 CATATG CA^TATG
2857 CATATG
PstI 1503 CTGCAG CTGCA^G
4946 CTGCAG
SapI 436 GAAGAGC GCTCTTC(1/4)
1231 GAAGAGC
SexAI 462 ACCAGGT A^CC[AT]GGT
712 ACCTGGT
TaqI 2908 TCGA T^CGA
3961 TCGA
TthHB8I 2908 TCGA T^CGA
3961 TCGA
XcmI 1439 CCAGGGTGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2992 CCATCCCAACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
AvrII BanII BbsI BbvCI BbvI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI CfoI Cfr13I
DdeI DpnII DraI DraIII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII