##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccII AccIII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII BbeI BbrPI BglII
BsaAI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI HaeII KasI
KspI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SnaBI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3791 GACGTC GACGT^C
Acc65I 2580 GGTACC G^GTACC
AccI 705 GTAGAC GT^[AC][GT]AC
AgeI 3852 ACCGGT A^CCGGT
Asp718I 2580 GGTACC G^GTACC
AvaI 1507 CCCGGG C^[CT]CG[GA]G
BamHI 3812 GGATCC G^GATCC
BbvCI 2010 GCTGAGG CCTCAGC(-5/-2)
BcgI 1700 CGAAGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6011 GTATCC GTATCC(6/5)
BglI 5475 GCCTTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 1507 CCCGGG C^[CT]CG[GA]G
BstAPI 3565 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2715 TTCGAA TT^CGAA
BstEII 3342 GGTGACC G^GT[ACGT]ACC
BstPI 3342 GGTGACC G^GT[ACGT]ACC
DraIII 2335 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3895 CCGTGG C^C[GA][CT]GG
EcoO65I 3342 GGTGACC G^GT[ACGT]ACC
EcoRI 2712 GAATTC G^AATTC
HpaI 428 GTTAAC GTT^AAC
KpnI 2580 GGTACC GGTAC^C
MfeI 670 CAATTG C^AATTG
MunI 670 CAATTG C^AATTG
NspV 2715 TTCGAA TT^CGAA
PinAI 3852 ACCGGT A^CCGGT
SacI 3035 GAGCTC GAGCT^C
SfuI 2715 TTCGAA TT^CGAA
SgrAI 3851 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1507 CCCGGG CCC^GGG
SspI 456 AATATT AAT^ATT
XmaCI 1507 CCCGGG C^CCGGG
XmaI 1507 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AclI 4916 AACGTT AA^CGTT
5116 AACGTT
AcyI 3791 GACGTC G[GA]^CG[CT]C
5739 GACGCC
AocI 2508 CCTCAGG CC^T[ACGT]AGG
4158 CCTGAGG
BanI 2580 GGTACC G^G[CT][GA]CC
4322 GGCACC
BcnI 1507 CCCGG CC^[GC]GG
1508 CCGGG
BsaHI 3791 GACGTC G[GA]^CG[CT]C
5739 GACGCC
BsaI 2394 GGTCTC GGTCTC(1/5)
2541 GGTCTC
BsaWI 2955 TCCGGT [AT]^CCGG[AT]
3852 ACCGGT
BsrFI 2768 GCCGGT [GA]^CCGG[CT]
3852 ACCGGT
BstXI 2260 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4705 CCAGAGATTTGG
Bsu36I 2508 CCTCAGG CC^T[ACGT]AGG
4158 CCTGAGG
Cfr10I 2768 GCCGGT [GA]^CCGG[CT]
3852 ACCGGT
DrdI 834 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3093 GACCCCTCAGTC
Eco81I 2508 CCTCAGG CC^T[ACGT]AGG
4158 CCTGAGG
EcoNI 3882 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4158 CCTGAGGAAGG
EcoRV 2094 GATATC GAT^ATC
4472 GATATC
EcoT22I 5516 ATGCAT ATGCA^T
6298 ATGCAT
Hin1I 3791 GACGTC G[GA]^CG[CT]C
5739 GACGCC
HindIII 5543 AAGCTT A^AGCTT
6250 AAGCTT
NciI 1507 CCCGG CC^[GC]GG
1508 CCGGG
NdeI 4560 CATATG CA^TATG
6423 CATATG
NsiI 5516 ATGCAT ATGCA^T
6298 ATGCAT
Psp1406I 4916 AACGTT AA^CGTT
5116 AACGTT
ScaI 819 AGTACT AGT^ACT
1878 AGTACT
SphI 1160 GCATGC GCATG^C
1904 GCATGC
StuI 593 AGGCCT AGG^CCT
944 AGGCCT
XcmI 1583 CCAAGAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4264 CCAACTTGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaII AvrII BalI
BanII BbsI BbvI BclI BfaI
BfrI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI