##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI Asp700I BciVI BfrI BglII
BsaBI BsiWI BssSI Bst1107I BstBI
BstZ17I CpoI DraIII EcoRI EcoRV
FseI HindIII HpaI KspI MamI
MluI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SfuI SgrAI SpeI
SphI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1268 TCCGGA T^CCGGA
AhdI 271 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1382 AGCGCT AGC^GCT
AspEI 271 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1467 CCTAGG C^CTAGG
BamHI 4616 GGATCC G^GATCC
BcgI 4790 CGAGCACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5280 TGATCA T^GATCA
BlnI 1467 CCTAGG C^CTAGG
BseAI 1268 TCCGGA T^CCGGA
Bsp1407I 875 TGTACA T^GTACA
BspDI 5235 ATCGAT AT^CGAT
BspEI 1268 TCCGGA T^CCGGA
BspHI 1106 TCATGA T^CATGA
BspLU11I 4995 ACATGT A^CATGT
BsrDI 395 CATTGC GCAATG(2/0)
BsrGI 875 TGTACA T^GTACA
BssHII 3826 GCGCGC G^CGCGC
ClaI 5235 ATCGAT AT^CGAT
DraI 5228 TTTAAA TTT^AAA
DrdI 2089 GACTCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2996 CGGCCG C^GGCCG
Eam1105I 271 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2996 CGGCCG C^GGCCG
Eco47III 1382 AGCGCT AGC^GCT
Eco52I 2996 CGGCCG C^GGCCG
FbaI 5280 TGATCA T^GATCA
HincII 1849 GTCAAC GT[CT]^[GA]AC
HindII 1849 GTCAAC GT[CT]^[GA]AC
MfeI 225 CAATTG C^AATTG
MroI 1268 TCCGGA T^CCGGA
MunI 225 CAATTG C^AATTG
PaeR7I 2340 CTCGAG C^TCGAG
RcaI 1106 TCATGA T^CATGA
SacI 1216 GAGCTC GAGCT^C
SnaBI 778 TACGTA TAC^GTA
SspBI 875 TGTACA T^GTACA
SspI 4979 AATATT AAT^ATT
XhoI 2340 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1639 GACGTC GACGT^C
2638 GACGTC
Acc65I 461 GGTACC G^GTACC
1496 GGTACC
AccI 1696 GTAGAC GT^[AC][GT]AC
5146 GTAGAC
Alw44I 793 GTGCAC G^TGCAC
5067 GTGCAC
ApaLI 793 GTGCAC G^TGCAC
5067 GTGCAC
Asp718I 461 GGTACC G^GTACC
1496 GGTACC
AspI 25 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
160 GACTTGGTC
AviII 214 TGCGCA TGC^GCA
536 TGCGCA
BbeI 2794 GGCGCC GGCGC^C
5155 GGCGCC
BbvCI 3043 GCTGAGG CCTCAGC(-5/-2)
3694 GCTGAGG
BglI 1752 GCCCATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4464 GCCACACAGGC
BlpI 2455 GCTGAGC GC^T[ACGT]AGC
3016 GCTCAGC
Bpu1102I 2455 GCTGAGC GC^T[ACGT]AGC
3016 GCTCAGC
BsaWI 1268 TCCGGA [AT]^CCGG[AT]
1499 ACCGGA
BsiEI 1236 CGACCG CG[GA][CT]^CG
2996 CGGCCG
BsmI 1601 GCATTC GAATGC(1/-1)
5206 GCATTC
BsrFI 2779 GCCGGC [GA]^CCGG[CT]
3340 GCCGGC
CelII 2455 GCTGAGC GC^T[ACGT]AGC
3016 GCTCAGC
Cfr10I 2779 GCCGGC [GA]^CCGG[CT]
3340 GCCGGC
EcoNI 1899 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2958 CCTGACTCAGG
EcoT22I 392 ATGCAT ATGCA^T
5204 ATGCAT
FspI 214 TGCGCA TGC^GCA
536 TGCGCA
KasI 2794 GGCGCC G^GCGCC
5155 GGCGCC
KpnI 461 GGTACC GGTAC^C
1496 GGTACC
NaeI 2779 GCCGGC GCC^GGC
3340 GCCGGC
NarI 2794 GGCGCC GG^CGCC
5155 GGCGCC
NgoMIV 2779 GCCGGC G^CCGGC
3340 GCCGGC
NsiI 392 ATGCAT ATGCA^T
5204 ATGCAT
PflFI 25 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
160 GACTTGGTC
SapI 922 GAAGAGC GCTCTTC(1/4)
2514 GCTCTTC
SexAI 1691 ACCAGGT A^CC[AT]GGT
2711 ACCTGGT
SfoI 2794 GGCGCC GGC^GCC
5155 GGCGCC
Tth111I 25 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
160 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
SmlI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI