##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AflII AscI Asp700I Asp718I
AspI AvrII BamHI BciVI BfrI
BlnI BsiWI BsmI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRV FseI KpnI KspI MfeI
MluI MunI NdeI NotI NruI
NspV PacI PflFI PflMI PmeI
PshAI PvuI RsrII SacII SapI
SbfI SfiI SfuI SgrAI SmaI
Sse8387I SspBI SwaI Tth111I Van91I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2175 GACGTC GACGT^C
AclI 4001 AACGTT AA^CGTT
Alw44I 550 GTGCAC G^TGCAC
ApaI 5290 GGGCCC GGGCC^C
ApaLI 550 GTGCAC G^TGCAC
AviII 7466 TGCGCA TGC^GCA
BbeI 1227 GGCGCC GGCGC^C
BbrPI 4295 CACGTG CAC^GTG
BglI 4982 GCCACCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2984 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 7266 CATTGC GCAATG(2/0)
BstAPI 163 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2243 GGTAACC G^GT[ACGT]ACC
BstPI 2243 GGTAACC G^GT[ACGT]ACC
DraIII 4295 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2243 GGTAACC G^GT[ACGT]ACC
EcoRI 6343 GAATTC G^AATTC
EcoT22I 3818 ATGCAT ATGCA^T
FspI 7466 TGCGCA TGC^GCA
HpaI 7371 GTTAAC GTT^AAC
KasI 1227 GGCGCC G^GCGCC
MamI 2984 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2220 GCCGGC GCC^GGC
NarI 1227 GGCGCC GG^CGCC
NgoMIV 2220 GCCGGC G^CCGGC
NsiI 3818 ATGCAT ATGCA^T
PmaCI 4295 CACGTG CAC^GTG
PmlI 4295 CACGTG CAC^GTG
Psp1406I 4001 AACGTT AA^CGTT
SalI 6874 GTCGAC G^TCGAC
SfoI 1227 GGCGCC GGC^GCC
SnaBI 6238 TACGTA TAC^GTA
SphI 5526 GCATGC GCATG^C
SspI 6611 AATATT AAT^ATT
StuI 565 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 846 TCCGGA T^CCGGA
1677 TCCGGA
AflIII 3011 ACATGT A^C[GA][CT]GT
4855 ACATGT
AgeI 507 ACCGGT A^CCGGT
6719 ACCGGT
Aor51HI 2196 AGCGCT AGC^GCT
6940 AGCGCT
BcgI 1259 CGAGCGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6037 CGAACCCAGTGC
BglII 2837 AGATCT A^GATCT
3874 AGATCT
BseAI 846 TCCGGA T^CCGGA
1677 TCCGGA
BsiEI 505 CGACCG CG[GA][CT]^CG
1114 CGACCG
BspEI 846 TCCGGA T^CCGGA
1677 TCCGGA
BspLU11I 3011 ACATGT A^CATGT
4855 ACATGT
BssSI 1216 CACGAG CACGAG(-5/-1)
4828 CACGAG
Eco47III 2196 AGCGCT AGC^GCT
6940 AGCGCT
HgaI 4513 GCGTC GACGC(5/10)
5633 GCGTC
MroI 846 TCCGGA T^CCGGA
1677 TCCGGA
NheI 989 GCTAGC G^CTAGC
1588 GCTAGC
PinAI 507 ACCGGT A^CCGGT
6719 ACCGGT
SpeI 5786 ACTAGT A^CTAGT
7498 ACTAGT
XbaI 253 TCTAGA T^CTAGA
3167 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApoI AseI AsnI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PleI
PpuMI PshBI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII