##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AflII AgeI Alw44I ApaI
ApaLI AscI AspI BalI BbrPI
BbvCI BcnI BfrI BsiWI BspDI
BsrBI BsrFI BstBI BstEII BstPI
Cfr10I ClaI CpoI DraIII EcoO65I
EcoRV FauI FseI HpaI KspI
MluI MluNI MscI NaeI NciI
NgoMIV NruI NspV PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacI SacII SalI SfuI SgrAI
SmaI StuI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 751 GGTACC G^GTACC
AccII 6279 CGCG CG^CG
AccIII 26 TCCGGA T^CCGGA
AclI 1340 AACGTT AA^CGTT
AcyI 2212 GGCGCC G[GA]^CG[CT]C
AhdI 4844 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2311 AGCGCT AGC^GCT
Asp718I 751 GGTACC G^GTACC
AspEI 4844 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2140 GAGCAC G[AT]GC[AT]^C
AviII 3336 TGCGCA TGC^GCA
AvrII 5576 CCTAGG C^CTAGG
BbeI 2212 GGCGCC GGCGC^C
BcgI 5950 CGATTGTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 442 TGATCA T^GATCA
BglI 79 GCCTTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5576 CCTAGG C^CTAGG
BsaHI 2212 GGCGCC G[GA]^CG[CT]C
BsaWI 26 TCCGGA [AT]^CCGG[AT]
BseAI 26 TCCGGA T^CCGGA
BsiEI 86 CGGCCG CG[GA][CT]^CG
BsiHKAI 2140 GAGCAC G[AT]GC[AT]^C
BspEI 26 TCCGGA T^CCGGA
BssHII 6278 GCGCGC G^CGCGC
BssSI 4883 CTCGTG CACGAG(-5/-1)
Bst1107I 583 GTATAC GTA^TAC
BstUI 6279 CGCG CG^CG
BstXI 5079 CCAGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 583 GTATAC GTA^TAC
EagI 86 CGGCCG C^GGCCG
Eam1105I 4844 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 86 CGGCCG C^GGCCG
Eco47III 2311 AGCGCT AGC^GCT
Eco52I 86 CGGCCG C^GGCCG
FbaI 442 TGATCA T^GATCA
FspI 3336 TGCGCA TGC^GCA
Hin1I 2212 GGCGCC G[GA]^CG[CT]C
KasI 2212 GGCGCC G^GCGCC
KpnI 751 GGTACC GGTAC^C
MfeI 4094 CAATTG C^AATTG
MroI 26 TCCGGA T^CCGGA
MspA1I 5934 CAGCTG C[AC]G^C[GT]G
MunI 4094 CAATTG C^AATTG
MvnI 6279 CGCG CG^CG
NarI 2212 GGCGCC GG^CGCC
NheI 3326 GCTAGC G^CTAGC
NotI 85 GCGGCCGC GC^GGCCGC
PacI 4133 TTAATTAA TTAAT^TAA
PflMI 4571 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 6385 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 6387 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1340 AACGTT AA^CGTT
PvuII 5934 CAGCTG CAG^CTG
SbfI 5846 CCTGCAGG CCTGCA^GG
ScaI 2462 AGTACT AGT^ACT
SexAI 6550 ACCTGGT A^CC[AT]GGT
SfiI 78 GGCCTTAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2212 GGCGCC GGC^GCC
SnaBI 879 TACGTA TAC^GTA
SpeI 1349 ACTAGT A^CTAGT
Sse8387I 5846 CCTGCAGG CCTGCA^GG
Van91I 4571 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 6011 TCTAGA T^CTAGA
XcmI 3115 CCACTTCTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 1509 ATTAAT AT^TAAT
4475 ATTAAT
AsnI 1509 ATTAAT AT^TAAT
4475 ATTAAT
BanII 4816 GGGCTC G[GA]GC[CT]^C
6102 GAGCCC
BciVI 1199 GGATAC GTATCC(6/5)
174 GTATCC
BlpI 2608 GCTTAGC GC^T[ACGT]AGC
5323 GCTGAGC
BpmI 2466 CTCCAG CTGGAG(16/14)
721 CTGGAG
Bpu1102I 2608 GCTTAGC GC^T[ACGT]AGC
5323 GCTGAGC
BsaBI 549 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1293 GATGAAAATC
BsmBI 52 GAGACG CGTCTC(1/5)
246 GAGACG
BspLU11I 6635 ACATGT A^CATGT
6701 ACATGT
BspMI 2787 ACCTGC ACCTGC(4/8)
2371 GCAGGT
BstAPI 2739 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3580 GCAGTGTATGC
CelII 2608 GCTTAGC GC^T[ACGT]AGC
5323 GCTGAGC
DraI 4245 TTTAAA TTT^AAA
6169 TTTAAA
EaeI 86 CGGCCG [CT]^GGCC[GA]
5198 TGGCCG
HaeII 2212 GGCGCC [GA]GCGC^[CT]
2311 AGCGCT
HapII 27 CCGG C^CGG
2435 CCGG
HgaI 248 GACGC GACGC(5/10)
4456 GCGTC
HindIII 3373 AAGCTT A^AGCTT
6323 AAGCTT
HpaII 27 CCGG C^CGG
2435 CCGG
MamI 549 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1293 GATGAAAATC
MspI 27 CCGG C^CGG
2435 CCGG
PaeR7I 1837 CTCGAG C^TCGAG
2400 CTCGAG
PshBI 1509 ATTAAT AT^TAAT
4475 ATTAAT
SapI 2232 GCTCTTC GCTCTTC(1/4)
3270 GCTCTTC
SphI 204 GCATGC GCATG^C
3339 GCATGC
XhoI 1837 CTCGAG C^TCGAG
2400 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AvaI AvaII BamHI BanI
BbsI BbvI BfaI BglII BmyI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI