##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AcsI AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BclI
BlnI BsaBI BsmI BspDI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoRI EcoRV
FbaI HpaI MamI MfeI MluI
MunI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SfuI
SnaBI SphI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2544 GACGTC GACGT^C
Acc65I 830 GGTACC G^GTACC
AccIII 2540 TCCGGA T^CCGGA
AflII 514 CTTAAG C^TTAAG
AocI 2596 CCTGAGG CC^T[ACGT]AGG
Asp718I 830 GGTACC G^GTACC
AspI 1732 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3774 CCTCAGC CCTCAGC(-5/-2)
BfrI 514 CTTAAG C^TTAAG
BglII 1190 AGATCT A^GATCT
BsaWI 2540 TCCGGA [AT]^CCGG[AT]
BseAI 2540 TCCGGA T^CCGGA
BsiWI 3979 CGTACG C^GTACG
Bsp1407I 2630 TGTACA T^GTACA
BspEI 2540 TCCGGA T^CCGGA
BspHI 1154 TCATGA T^CATGA
BsrDI 1554 CATTGC GCAATG(2/0)
BsrGI 2630 TGTACA T^GTACA
Bsu36I 2596 CCTGAGG CC^T[ACGT]AGG
DraIII 1093 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3217 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2596 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2985 ATGCAT ATGCA^T
FseI 125 GGCCGGCC GGCCGG^CC
HindIII 12 AAGCTT A^AGCTT
KpnI 830 GGTACC GGTAC^C
MroI 2540 TCCGGA T^CCGGA
NsiI 2985 ATGCAT ATGCA^T
PflFI 1732 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1154 TCATGA T^CATGA
SalI 3510 GTCGAC G^TCGAC
SbfI 2607 CCTGCAGG CCTGCA^GG
ScaI 1676 AGTACT AGT^ACT
SexAI 3899 ACCTGGT A^CC[AT]GGT
SgrAI 98 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3147 ACTAGT A^CTAGT
Sse8387I 2607 CCTGCAGG CCTGCA^GG
SspBI 2630 TGTACA T^GTACA
Tth111I 1732 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2075 ACGTGT A^C[GA][CT]GT
2195 ACGTGT
BamHI 529 GGATCC G^GATCC
4815 GGATCC
BbrPI 1657 CACGTG CAC^GTG
4000 CACGTG
BcgI 4771 CGAGCGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3305 GCAAGGCCATCG
BciVI 3153 GGATAC GTATCC(6/5)
1670 GTATCC
BssHII 915 GCGCGC G^CGCGC
979 GCGCGC
HincII 3196 GTCAAC GT[CT]^[GA]AC
3510 GTCGAC
HindII 3196 GTCAAC GT[CT]^[GA]AC
3510 GTCGAC
MseI 515 TTAA T^TAA
5162 TTAA
NheI 2587 GCTAGC G^CTAGC
4394 GCTAGC
NspI 1523 ACATGC [GA]CATG^[CT]
4967 GCATGT
PflMI 1212 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCAGTACTTGG
PmaCI 1657 CACGTG CAC^GTG
4000 CACGTG
PmlI 1657 CACGTG CAC^GTG
4000 CACGTG
SfiI 1428 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3723 GGCCGCGGTGGCC
TfiI 4287 GATTC G^A[AT]TC
4792 GAATC
Tru9I 515 TTAA T^TAA
5162 TTAA
Tsp509I 2038 AATT ^AATT
3282 AATT
Van91I 1212 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCAGTACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI