##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI BalI
BanI BbeI BbrPI BcgI BclI
BglI BsaAI BsaBI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV FbaI FseI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 230 GACGTC GACGT^C
AccI 207 GTCTAC GT^[AC][GT]AC
AcyI 230 GACGTC G[GA]^CG[CT]C
AfaI 57 GTAC GT^AC
Aor51HI 602 AGCGCT AGC^GCT
ApaI 26 GGGCCC GGGCC^C
AseI 1826 ATTAAT AT^TAAT
AsnI 1826 ATTAAT AT^TAAT
AviII 863 TGCGCA TGC^GCA
AvrII 1812 CCTAGG C^CTAGG
BamHI 4094 GGATCC G^GATCC
BbsI 2778 GAAGAC GAAGAC(2/6)
BciVI 2329 GTATCC GTATCC(6/5)
BcnI 986 CCGGG CC^[GC]GG
BlnI 1812 CCTAGG C^CTAGG
BlpI 2378 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2378 GCTAAGC GC^T[ACGT]AGC
BpuAI 2778 GAAGAC GAAGAC(2/6)
BsaHI 230 GACGTC G[GA]^CG[CT]C
BsmBI 3705 GAGACG CGTCTC(1/5)
BsmI 2785 GAATGC GAATGC(1/-1)
Bsp1407I 56 TGTACA T^GTACA
BspHI 3982 TCATGA T^CATGA
BsrFI 887 ACCGGC [GA]^CCGG[CT]
BsrGI 56 TGTACA T^GTACA
CelII 2378 GCTAAGC GC^T[ACGT]AGC
Cfr10I 887 ACCGGC [GA]^CCGG[CT]
DraIII 503 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2687 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 780 CGGCCA [CT]^GGCC[GA]
Eco47III 602 AGCGCT AGC^GCT
EcoNI 3451 CCTAAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 863 TGCGCA TGC^GCA
Hin1I 230 GACGTC G[GA]^CG[CT]C
NciI 986 CCGGG CC^[GC]GG
PshBI 1826 ATTAAT AT^TAAT
RcaI 3982 TCATGA T^CATGA
RsaI 57 GTAC GT^AC
SacI 641 GAGCTC GAGCT^C
SexAI 1122 ACCAGGT A^CC[AT]GGT
SgrAI 886 CACCGGCG C[GA]^CCGG[CT]G
SspBI 56 TGTACA T^GTACA
StuI 102 AGGCCT AGG^CCT
TaqI 621 TCGA T^CGA
TthHB8I 621 TCGA T^CGA
XcmI 2232 CCAGTGAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 1704 CTTAAG C^TTAAG
3827 CTTAAG
AlwNI 236 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
3223 CAGAATCTG
BbvCI 453 CCTCAGC CCTCAGC(-5/-2)
3731 CCTCAGC
BfrI 1704 CTTAAG C^TTAAG
3827 CTTAAG
BglII 1750 AGATCT A^GATCT
2028 AGATCT
BpmI 308 CTCCAG CTGGAG(16/14)
3437 CTGGAG
BsaI 1183 GAGACC GGTCTC(1/5)
3538 GAGACC
BsaWI 33 ACCGGA [AT]^CCGG[AT]
635 ACCGGA
BsrBI 907 GAGCGG CCGCTC(-3/-3)
991 GAGCGG
BstAPI 166 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
839 GCACTGACTGC
DsaI 3104 CCATGG C^C[GA][CT]GG
3240 CCATGG
EcoT22I 2388 ATGCAT ATGCA^T
2971 ATGCAT
FauI 3571 CCCGC CCCGC(4/6)
723 GCGGG
HaeII 602 AGCGCT [GA]GCGC^[CT]
740 AGCGCC
HgaI 573 GCGTC GACGC(5/10)
825 GCGTC
HincII 1165 GTCAAC GT[CT]^[GA]AC
4104 GTTGAC
HindII 1165 GTCAAC GT[CT]^[GA]AC
4104 GTTGAC
MspA1I 666 CCGCTG C[AC]G^C[GT]G
721 CAGCGG
NcoI 3104 CCATGG C^CATGG
3240 CCATGG
NsiI 2388 ATGCAT ATGCA^T
2971 ATGCAT
PflMI 1187 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3222 CCAGAATCTGG
Van91I 1187 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3222 CCAGAATCTGG
XbaI 1179 TCTAGA T^CTAGA
2861 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
AocI ApoI AspHI AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII