##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AviII AvrII BalI
BbeI BcgI BfrI BlnI BlpI
Bpu1102I BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspEI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII CpoI DraIII DrdI
Eco47III Eco81I EcoNI EcoRV FseI
FspI HaeII HindIII KasI KpnI
MluI MluNI MroI MscI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PvuI RsrII SalI SapI SbfI
SfiI SfoI SfuI SgrAI Sse8387I
SspBI StuI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AclI 4165 AACGTT AA^CGTT
AcyI 69 GACGCC G[GA]^CG[CT]C
AhdI 2056 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2634 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 915 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2056 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 453 GAGCTC G[AT]GC[AT]^C
AvaI 90 CCCGGG C^[CT]CG[GA]G
BanI 86 GGTGCC G^G[CT][GA]CC
BbrPI 1045 CACGTG CAC^GTG
BciVI 2825 GGATAC GTATCC(6/5)
BclI 3999 TGATCA T^GATCA
BglI 72 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 413 AGATCT A^GATCT
BsaHI 69 GACGCC G[GA]^CG[CT]C
BsaI 3393 GGTCTC GGTCTC(1/5)
BsaWI 1495 ACCGGA [AT]^CCGG[AT]
BsiHKAI 453 GAGCTC G[AT]GC[AT]^C
BsoBI 90 CCCGGG C^[CT]CG[GA]G
BspDI 1557 ATCGAT AT^CGAT
BspLU11I 4084 ACATGT A^CATGT
BsrFI 744 GCCGGT [GA]^CCGG[CT]
BssSI 3410 CTCGTG CACGAG(-5/-1)
BstEII 3842 GGTAACC G^GT[ACGT]ACC
BstPI 3842 GGTAACC G^GT[ACGT]ACC
Cfr10I 744 GCCGGT [GA]^CCGG[CT]
ClaI 1557 ATCGAT AT^CGAT
DraII 3543 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2056 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 3543 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3842 GGTAACC G^GT[ACGT]ACC
FbaI 3999 TGATCA T^GATCA
Hin1I 69 GACGCC G[GA]^CG[CT]C
HpaI 4080 GTTAAC GTT^AAC
NheI 3808 GCTAGC G^CTAGC
PflMI 1167 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1045 CACGTG CAC^GTG
PmlI 1045 CACGTG CAC^GTG
PpuMI 3543 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 4165 AACGTT AA^CGTT
PvuII 1783 CAGCTG CAG^CTG
SacI 453 GAGCTC GAGCT^C
ScaI 2134 AGTACT AGT^ACT
SexAI 1526 ACCAGGT A^CC[AT]GGT
SmaI 90 CCCGGG CCC^GGG
SnaBI 3884 TACGTA TAC^GTA
SpeI 1290 ACTAGT A^CTAGT
SphI 4443 GCATGC GCATG^C
SwaI 2346 ATTTAAAT ATTT^AAAT
Van91I 1167 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 90 CCCGGG C^CCGGG
XmaI 90 CCCGGG C^CCGGG
XmnI 915 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 748 GTAGAC GT^[AC][GT]AC
1360 GTAGAC
AflIII 650 ACACGT A^C[GA][CT]GT
4084 ACATGT
AspI 1072 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3196 GACACAGTC
BamHI 587 GGATCC G^GATCC
2707 GGATCC
BbsI 565 GAAGAC GAAGAC(2/6)
858 GAAGAC
BbvCI 23 CCTCAGC CCTCAGC(-5/-2)
3292 CCTCAGC
BpuAI 565 GAAGAC GAAGAC(2/6)
858 GAAGAC
BsaBI 1560 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1573 GATTTTTATC
BseRI 1195 GAGGAG GAGGAG(10/8)
1249 GAGGAG
BsiEI 35 CGGCCG CG[GA][CT]^CG
3472 CGGCCG
BspHI 1001 TCATGA T^CATGA
2247 TCATGA
BspMI 118 ACCTGC ACCTGC(4/8)
3087 ACCTGC
BstXI 3217 CCAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3435 CCAAAATCCTGG
EagI 35 CGGCCG C^GGCCG
3472 CGGCCG
EarI 246 GAAGAG CTCTTC(1/4)
1005 GAAGAG
EclXI 35 CGGCCG C^GGCCG
3472 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
3472 CGGCCG
EcoT22I 969 ATGCAT ATGCA^T
1858 ATGCAT
HgaI 69 GACGC GACGC(5/10)
4370 GCGTC
Ksp632I 246 GAAGAG CTCTTC(1/4)
1005 GAAGAG
KspI 147 CCGCGG CCGC^GG
166 CCGCGG
MamI 1560 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1573 GATTTTTATC
MfeI 910 CAATTG C^AATTG
3583 CAATTG
MunI 910 CAATTG C^AATTG
3583 CAATTG
NcoI 1873 CCATGG C^CATGG
2671 CCATGG
NsiI 969 ATGCAT ATGCA^T
1858 ATGCAT
PflFI 1072 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3196 GACACAGTC
RcaI 1001 TCATGA T^CATGA
2247 TCATGA
SacII 147 CCGCGG CCGC^GG
166 CCGCGG
SfaNI 465 GATGC GCATC(5/9)
1555 GCATC
Tth111I 1072 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3196 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII