##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BclI BfrI BlnI
BsaBI BspDI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco47III Eco81I EcoO65I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MamI MluI
MluNI MscI NcoI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PstI PvuI RsrII SacI SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 3717 GTCTAC GT^[AC][GT]AC
AccIII 460 TCCGGA T^CCGGA
ApaI 206 GGGCCC GGGCC^C
AseI 2765 ATTAAT AT^TAAT
AsnI 2765 ATTAAT AT^TAAT
AspHI 1157 GAGCAC G[AT]GC[AT]^C
BcgI 911 GCAGACCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4025 GTATCC GTATCC(6/5)
BglI 33 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1038 AGATCT A^GATCT
BlpI 3595 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3595 GCTCAGC GC^T[ACGT]AGC
BsaWI 460 TCCGGA [AT]^CCGG[AT]
BseAI 460 TCCGGA T^CCGGA
BsiEI 731 CGGCCG CG[GA][CT]^CG
BsiHKAI 1157 GAGCAC G[AT]GC[AT]^C
BsiWI 1557 CGTACG C^GTACG
BspEI 460 TCCGGA T^CCGGA
BsrDI 2601 GCAATG GCAATG(2/0)
BssHII 313 GCGCGC G^CGCGC
CelII 3595 GCTCAGC GC^T[ACGT]AGC
DrdI 1874 GACAACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 731 CGGCCG C^GGCCG
EclXI 731 CGGCCG C^GGCCG
Eco52I 731 CGGCCG C^GGCCG
EcoNI 535 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 234 CCGCGG CCGC^GG
MfeI 3325 CAATTG C^AATTG
MroI 460 TCCGGA T^CCGGA
MunI 3325 CAATTG C^AATTG
NaeI 646 GCCGGC GCC^GGC
NdeI 4075 CATATG CA^TATG
NgoMIV 646 GCCGGC G^CCGGC
PshBI 2765 ATTAAT AT^TAAT
SacII 234 CCGCGG CCGC^GG
SapI 4048 GCTCTTC GCTCTTC(1/4)
ScaI 1902 AGTACT AGT^ACT
SpeI 2309 ACTAGT A^CTAGT
XcmI 1579 CCAGTTTACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 2045 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2540 GAAAGAATTC
BamHI 2393 GGATCC G^GATCC
3097 GGATCC
BbrPI 703 CACGTG CAC^GTG
1328 CACGTG
BbsI 755 GAAGAC GAAGAC(2/6)
2742 GAAGAC
BbvCI 529 CCTCAGC CCTCAGC(-5/-2)
3981 GCTGAGG
BpuAI 755 GAAGAC GAAGAC(2/6)
2742 GAAGAC
BsaAI 703 CACGTG [CT]AC^GT[GA]
1328 CACGTG
BsmBI 2068 CGTCTC CGTCTC(1/5)
449 GAGACG
Bsp1407I 990 TGTACA T^GTACA
2493 TGTACA
BspMI 564 ACCTGC ACCTGC(4/8)
3992 GCAGGT
BsrBI 93 GAGCGG CCGCTC(-3/-3)
179 GAGCGG
BsrGI 990 TGTACA T^GTACA
2493 TGTACA
BstAPI 1260 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3743 GCATTAGATGC
DraI 2965 TTTAAA TTT^AAA
4224 TTTAAA
DraIII 1137 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1636 CACCTTGTG
EaeI 246 TGGCCG [CT]^GGCC[GA]
731 CGGCCG
EarI 4049 CTCTTC CTCTTC(1/4)
2696 GAAGAG
EcoRI 929 GAATTC G^AATTC
2544 GAATTC
EcoRV 2325 GATATC GAT^ATC
2333 GATATC
HindIII 2016 AAGCTT A^AGCTT
2908 AAGCTT
Ksp632I 4049 CTCTTC CTCTTC(1/4)
2696 GAAGAG
PmaCI 703 CACGTG CAC^GTG
1328 CACGTG
PmlI 703 CACGTG CAC^GTG
1328 CACGTG
SmaI 319 CCCGGG CCC^GGG
3264 CCCGGG
SmlI 1943 CTTGAG C^T[CT][GA]AG
2509 CTTGAG
SspBI 990 TGTACA T^GTACA
2493 TGTACA
XmaCI 319 CCCGGG C^CCGGG
3264 CCCGGG
XmaI 319 CCCGGG C^CCGGG
3264 CCCGGG
XmnI 2045 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2540 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII