##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BbrPI BcgI
BciVI BclI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI DraIII
DrdI Eam1105I Eco47III EcoO65I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NotI NruI NspV PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI SacI SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I StuI Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 284 GGCGCC G[GA]^CG[CT]C
AflII 3307 CTTAAG C^TTAAG
AlwNI 3640 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2092 CCTCAGG CC^T[ACGT]AGG
AspHI 2531 GAGCAC G[AT]GC[AT]^C
AspI 2897 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2958 GGATCC G^GATCC
BbeI 284 GGCGCC GGCGC^C
BfrI 3307 CTTAAG C^TTAAG
BglI 115 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1034 AGATCT A^GATCT
BsaHI 284 GGCGCC G[GA]^CG[CT]C
BsiEI 47 CGGCCG CG[GA][CT]^CG
BsiHKAI 2531 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1805 TGTACA T^GTACA
BspMI 3319 ACCTGC ACCTGC(4/8)
BsrBI 199 CCGCTC CCGCTC(-3/-3)
BsrGI 1805 TGTACA T^GTACA
BssHII 276 GCGCGC G^CGCGC
BssSI 2534 CACGAG CACGAG(-5/-1)
BstXI 2152 CCAGTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2092 CCTCAGG CC^T[ACGT]AGG
CpoI 125 CGGACCG CG^G[AT]CCG
EagI 47 CGGCCG C^GGCCG
EclXI 47 CGGCCG C^GGCCG
Eco52I 47 CGGCCG C^GGCCG
Eco81I 2092 CCTCAGG CC^T[ACGT]AGG
EcoRI 3871 GAATTC G^AATTC
HaeII 284 GGCGCC [GA]GCGC^[CT]
HgaI 1358 GACGC GACGC(5/10)
Hin1I 284 GGCGCC G[GA]^CG[CT]C
KasI 284 GGCGCC G^GCGCC
KspI 300 CCGCGG CCGC^GG
MaeII 421 ACGT A^CGT
NarI 284 GGCGCC GG^CGCC
NdeI 3411 CATATG CA^TATG
PacI 3426 TTAATTAA TTAAT^TAA
PaeR7I 2824 CTCGAG C^TCGAG
PflFI 2897 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 136 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3728 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 125 CGGACCG CG^G[AT]CCG
SacII 300 CCGCGG CCGC^GG
SapI 1187 GAAGAGC GCTCTTC(1/4)
ScaI 1873 AGTACT AGT^ACT
SfoI 284 GGCGCC GGC^GCC
SpeI 2978 ACTAGT A^CTAGT
SphI 1523 GCATGC GCATG^C
SspBI 1805 TGTACA T^GTACA
SwaI 3371 ATTTAAAT ATTT^AAAT
Tth111I 2897 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2824 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 2866 ACATGT A^C[GA][CT]GT
3990 ACATGT
AseI 2573 ATTAAT AT^TAAT
3293 ATTAAT
AsnI 2573 ATTAAT AT^TAAT
3293 ATTAAT
BanI 284 GGCGCC G^G[CT][GA]CC
1166 GGCACC
BanII 295 GAGCCC G[GA]GC[CT]^C
1961 GGGCTC
BbsI 2787 GTCTTC GAAGAC(2/6)
3994 GTCTTC
BlpI 1963 GCTCAGC GC^T[ACGT]AGC
2528 GCTGAGC
Bpu1102I 1963 GCTCAGC GC^T[ACGT]AGC
2528 GCTGAGC
BpuAI 2787 GTCTTC GAAGAC(2/6)
3994 GTCTTC
BsgI 3457 CTGCAC GTGCAG(16/14)
392 GTGCAG
BspLU11I 2866 ACATGT A^CATGT
3990 ACATGT
BsrFI 49 GCCGGC [GA]^CCGG[CT]
115 GCCGGC
CelII 1963 GCTCAGC GC^T[ACGT]AGC
2528 GCTGAGC
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
115 GCCGGC
DraII 136 GGGTCCC [GA]G^G[ACGT]CC[CT]
1692 GGGGCCT
DsaI 300 CCGCGG C^C[GA][CT]GG
353 CCGTGG
EaeI 47 CGGCCG [CT]^GGCC[GA]
350 TGGCCG
EcoNI 320 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3999 CCTTTTTGAGG
EcoO109I 136 GGGTCCC [GA]G^G[ACGT]CC[CT]
1692 GGGGCCT
EcoRV 1278 GATATC GAT^ATC
3355 GATATC
EcoT14I 2274 CCAAGG C^C[AT][AT]GG
2654 CCTTGG
EcoT22I 699 ATGCAT ATGCA^T
3170 ATGCAT
NaeI 49 GCCGGC GCC^GGC
115 GCCGGC
NgoMIV 49 GCCGGC G^CCGGC
115 GCCGGC
NheI 1361 GCTAGC G^CTAGC
2268 GCTAGC
NsiI 699 ATGCAT ATGCA^T
3170 ATGCAT
PshBI 2573 ATTAAT AT^TAAT
3293 ATTAAT
SmlI 2824 CTCGAG C^T[CT][GA]AG
3307 CTTAAG
SspI 2622 AATATT AAT^ATT
3528 AATATT
StyI 2274 CCAAGG C^C[AT][AT]GG
2654 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII