##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspI AvrII BalI BbeI
BbsI BcgI BglI BglII BlnI
BpuAI BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DsaI EagI EclXI
Eco47III Eco52I EcoO65I FseI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SfiI SfoI SfuI SgrAI SnaBI
StuI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 1577 CGCG CG^CG
AcyI 1180 GGCGTC G[GA]^CG[CT]C
AhdI 3393 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3393 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 55 TGCGCA TGC^GCA
BamHI 3135 GGATCC G^GATCC
BciVI 3412 GGATAC GTATCC(6/5)
BclI 1583 TGATCA T^GATCA
BsaHI 1180 GGCGTC G[GA]^CG[CT]C
BsaWI 73 ACCGGA [AT]^CCGG[AT]
BseRI 2956 GAGGAG GAGGAG(10/8)
BsmI 1247 GCATTC GAATGC(1/-1)
BspLU11I 2742 ACATGT A^CATGT
BspMI 2704 ACCTGC ACCTGC(4/8)
BsrFI 527 ACCGGC [GA]^CCGG[CT]
BssSI 1552 CACGAG CACGAG(-5/-1)
BstUI 1577 CGCG CG^CG
Cfr10I 527 ACCGGC [GA]^CCGG[CT]
DrdI 587 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 35 TGGCCG [CT]^GGCC[GA]
Eam1105I 3393 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1687 GATATC GAT^ATC
EcoT14I 1032 CCTTGG C^C[AT][AT]GG
EcoT22I 599 ATGCAT ATGCA^T
FbaI 1583 TGATCA T^GATCA
FspI 55 TGCGCA TGC^GCA
HaeII 61 GGCGCT [GA]GCGC^[CT]
Hin1I 1180 GGCGTC G[GA]^CG[CT]C
HincII 1776 GTTAAC GT[CT]^[GA]AC
HindII 1776 GTTAAC GT[CT]^[GA]AC
HindIII 2614 AAGCTT A^AGCTT
HpaI 1776 GTTAAC GTT^AAC
MvnI 1577 CGCG CG^CG
NsiI 599 ATGCAT ATGCA^T
SbfI 1193 CCTGCAGG CCTGCA^GG
ScaI 169 AGTACT AGT^ACT
SexAI 29 ACCTGGT A^CC[AT]GGT
SpeI 2527 ACTAGT A^CTAGT
Sse8387I 1193 CCTGCAGG CCTGCA^GG
StyI 1032 CCTTGG C^C[AT][AT]GG
SwaI 2217 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 2849 CTTAAG C^TTAAG
2891 CTTAAG
AflIII 1078 ACACGT A^C[GA][CT]GT
2742 ACATGT
AseI 852 ATTAAT AT^TAAT
3533 ATTAAT
AsnI 852 ATTAAT AT^TAAT
3533 ATTAAT
Asp700I 412 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2924 GAATCCTTTC
AspHI 673 GAGCAC G[AT]GC[AT]^C
1129 GAGCAC
BanI 294 GGTGCC G^G[CT][GA]CC
1448 GGCACC
BanII 1507 GGGCTC G[GA]GC[CT]^C
1546 GGGCTC
BbrPI 1000 CACGTG CAC^GTG
1079 CACGTG
BfrI 2849 CTTAAG C^TTAAG
2891 CTTAAG
BsaAI 1000 CACGTG [CT]AC^GT[GA]
1079 CACGTG
BsaI 1491 GAGACC GGTCTC(1/5)
3629 GAGACC
BsiHKAI 673 GAGCAC G[AT]GC[AT]^C
1129 GAGCAC
Bsp1407I 1676 TGTACA T^GTACA
3440 TGTACA
BspHI 2083 TCATGA T^CATGA
3162 TCATGA
BsrBI 81 GAGCGG CCGCTC(-3/-3)
1630 GAGCGG
BsrGI 1676 TGTACA T^GTACA
3440 TGTACA
BstAPI 801 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1515 GCAGAGCCTGC
EcoNI 13 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1452 CCTGGCTGAGG
PmaCI 1000 CACGTG CAC^GTG
1079 CACGTG
PmlI 1000 CACGTG CAC^GTG
1079 CACGTG
PpuMI 26 AGGACCT [GA]G^G[AT]CC[CT]
2256 AGGACCC
PshBI 852 ATTAAT AT^TAAT
3533 ATTAAT
RcaI 2083 TCATGA T^CATGA
3162 TCATGA
SapI 627 GCTCTTC GCTCTTC(1/4)
1788 GCTCTTC
SmaI 290 CCCGGG CCC^GGG
3752 CCCGGG
SphI 2393 GCATGC GCATG^C
3699 GCATGC
SspBI 1676 TGTACA T^GTACA
3440 TGTACA
XcmI 509 CCAATTCCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1041 CCAAGAGGACAATGG
XmaCI 290 CCCGGG C^CCGGG
3752 CCCGGG
XmaI 290 CCCGGG C^CCGGG
3752 CCCGGG
XmnI 412 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2924 GAATCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaI
AvaII BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoRI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII