##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BglI
BlnI BlpI Bpu1102I BpuAI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssSI Bst1107I BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
KasI KpnI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI Sse8387I StuI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 973 GTCTAC GT^[AC][GT]AC
AcyI 3291 GACGCC G[GA]^CG[CT]C
AflII 3952 CTTAAG C^TTAAG
Alw44I 1407 GTGCAC G^TGCAC
ApaI 109 GGGCCC GGGCC^C
ApaLI 1407 GTGCAC G^TGCAC
AseI 2354 ATTAAT AT^TAAT
AsnI 2354 ATTAAT AT^TAAT
BanII 109 GGGCCC G[GA]GC[CT]^C
BfrI 3952 CTTAAG C^TTAAG
BglII 1011 AGATCT A^GATCT
BpmI 1833 CTGGAG CTGGAG(16/14)
BsaAI 1000 TACGTG [CT]AC^GT[GA]
BsaHI 3291 GACGCC G[GA]^CG[CT]C
BsaI 3930 GGTCTC GGTCTC(1/5)
BsmBI 3483 GAGACG CGTCTC(1/5)
BspHI 1198 TCATGA T^CATGA
BspLU11I 835 ACATGT A^CATGT
BssHII 93 GCGCGC G^CGCGC
BstAPI 3600 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1006 TTCGAA TT^CGAA
BstEII 1912 GGTCACC G^GT[ACGT]ACC
BstPI 1912 GGTCACC G^GT[ACGT]ACC
DsaI 1969 CCATGG C^C[GA][CT]GG
EcoO65I 1912 GGTCACC G^GT[ACGT]ACC
HaeII 31 AGCGCC [GA]GCGC^[CT]
Hin1I 3291 GACGCC G[GA]^CG[CT]C
HindIII 3532 AAGCTT A^AGCTT
HpaI 2567 GTTAAC GTT^AAC
MfeI 1363 CAATTG C^AATTG
MspA1I 3673 CAGCTG C[AC]G^C[GT]G
MunI 1363 CAATTG C^AATTG
NcoI 1969 CCATGG C^CATGG
NspV 1006 TTCGAA TT^CGAA
PshAI 1982 GACCGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2354 ATTAAT AT^TAAT
PvuII 3673 CAGCTG CAG^CTG
RcaI 1198 TCATGA T^CATGA
SfuI 1006 TTCGAA TT^CGAA
SmaI 2083 CCCGGG CCC^GGG
SphI 392 GCATGC GCATG^C
SspI 3973 AATATT AAT^ATT
SwaI 2251 ATTTAAAT ATTT^AAAT
XmaCI 2083 CCCGGG C^CCGGG
XmaI 2083 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 94 CGCG CG^CG
444 CGCG
AflIII 835 ACATGT A^C[GA][CT]GT
1001 ACGTGT
AocI 640 CCTCAGG CC^T[ACGT]AGG
3363 CCTGAGG
Asp700I 2624 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4415 GAAGTCTTTC
AvaII 39 GGTCC G^G[AT]CC
3834 GGTCC
BclI 2026 TGATCA T^GATCA
3041 TGATCA
BsaBI 860 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1053 GATCCAGATC
Bsp1407I 2365 TGTACA T^GTACA
3057 TGTACA
BsrGI 2365 TGTACA T^GTACA
3057 TGTACA
BstUI 94 CGCG CG^CG
444 CGCG
BstXI 284 CCATAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3205 CCATTAAGTTGG
Bsu36I 640 CCTCAGG CC^T[ACGT]AGG
3363 CCTGAGG
DraI 2252 TTTAAA TTT^AAA
4194 TTTAAA
Eco81I 640 CCTCAGG CC^T[ACGT]AGG
3363 CCTGAGG
EcoNI 636 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2334 CCTGGTAAAGG
FbaI 2026 TGATCA T^GATCA
3041 TGATCA
HgaI 3291 GACGC GACGC(5/10)
3485 GACGC
MamI 860 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1053 GATCCAGATC
MvnI 94 CGCG CG^CG
444 CGCG
NspI 392 GCATGC [GA]CATG^[CT]
835 ACATGT
PpuMI 38 AGGTCCT [GA]G^G[AT]CC[CT]
3833 GGGTCCT
SapI 521 GAAGAGC GCTCTTC(1/4)
1931 GAAGAGC
SpeI 1638 ACTAGT A^CTAGT
4199 ACTAGT
SspBI 2365 TGTACA T^GTACA
3057 TGTACA
XmnI 2624 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4415 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI BalI
BanI BbvI BciVI BcnI BfaI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PaeR7I PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII