##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI AspI AviII BbeI BbrPI
BfrI BsaBI BsaHI BsiWI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EcoRV EcoT22I FspI Hin1I HincII
HindII HindIII HpaI KasI MamI
MfeI MluI MseI MunI NarI
NcoI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tru9I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1402 GGTACC G^GTACC
Aor51HI 3286 AGCGCT AGC^GCT
Asp718I 1402 GGTACC G^GTACC
AspHI 1153 GTGCTC G[AT]GC[AT]^C
AvrII 3910 CCTAGG C^CTAGG
BcgI 3816 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 23 AGATCT A^GATCT
BlnI 3910 CCTAGG C^CTAGG
BlpI 3541 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3541 GCTGAGC GC^T[ACGT]AGC
BsaI 487 GGTCTC GGTCTC(1/5)
BsiHKAI 1153 GTGCTC G[AT]GC[AT]^C
BsmBI 2334 CGTCTC CGTCTC(1/5)
Bsp1407I 82 TGTACA T^GTACA
BsrGI 82 TGTACA T^GTACA
BssSI 2284 CTCGTG CACGAG(-5/-1)
BstEII 3877 GGTCACC G^GT[ACGT]ACC
BstPI 3877 GGTCACC G^GT[ACGT]ACC
CelII 3541 GCTGAGC GC^T[ACGT]AGC
DrdI 2547 GACCGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 88 GAAGAG CTCTTC(1/4)
Eco47III 3286 AGCGCT AGC^GCT
EcoO65I 3877 GGTCACC G^GT[ACGT]ACC
KpnI 1402 GGTACC GGTAC^C
Ksp632I 88 GAAGAG CTCTTC(1/4)
KspI 2077 CCGCGG CCGC^GG
NdeI 3767 CATATG CA^TATG
NheI 1950 GCTAGC G^CTAGC
PaeR7I 3248 CTCGAG C^TCGAG
SacII 2077 CCGCGG CCGC^GG
SfiI 1674 GGCCCGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1208 GCATGC GCATG^C
SspBI 82 TGTACA T^GTACA
SspI 1478 AATATT AAT^ATT
XhoI 3248 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 37 TCCGGA T^CCGGA
2004 TCCGGA
AcsI 3469 GAATTC [GA]^AATT[CT]
3640 GAATTC
AocI 268 CCTGAGG CC^T[ACGT]AGG
3610 CCTGAGG
ApoI 3469 GAATTC [GA]^AATT[CT]
3640 GAATTC
Asp700I 2008 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3133 GAATCACTTC
BciVI 3066 GGATAC GTATCC(6/5)
3121 GTATCC
BclI 120 TGATCA T^GATCA
1337 TGATCA
BsaAI 439 TACGTG [CT]AC^GT[GA]
1702 TACGTG
BseAI 37 TCCGGA T^CCGGA
2004 TCCGGA
BsmI 1651 GAATGC GAATGC(1/-1)
1853 GAATGC
BspEI 37 TCCGGA T^CCGGA
2004 TCCGGA
BsrDI 1109 GCAATG GCAATG(2/0)
1664 GCAATG
BstAPI 952 GCATACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2563 GCACCCTCTGC
BstXI 3267 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3597 CCATGTTACTGG
Bsu36I 268 CCTGAGG CC^T[ACGT]AGG
3610 CCTGAGG
EagI 335 CGGCCG C^GGCCG
339 CGGCCG
EclXI 335 CGGCCG C^GGCCG
339 CGGCCG
Eco52I 335 CGGCCG C^GGCCG
339 CGGCCG
Eco81I 268 CCTGAGG CC^T[ACGT]AGG
3610 CCTGAGG
EcoRI 3469 GAATTC G^AATTC
3640 GAATTC
FbaI 120 TGATCA T^GATCA
1337 TGATCA
FseI 336 GGCCGGCC GGCCGG^CC
340 GGCCGGCC
MroI 37 TCCGGA T^CCGGA
2004 TCCGGA
StuI 2250 AGGCCT AGG^CCT
3228 AGGCCT
XmnI 2008 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3133 GAATCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI