##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
AscI AseI AsnI AspI AviII
AvrII BclI BglII BlnI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DrdI
Eco81I EcoNI EcoRI EcoT22I FbaI
FseI FspI HpaI MamI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SapI SbfI
SfiI SfuI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 2313 CGCG CG^CG
AhdI 1727 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1404 GTGCAC G^TGCAC
AlwNI 946 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1194 AGCGCT AGC^GCT
ApaI 2633 GGGCCC GGGCC^C
ApaLI 1404 GTGCAC G^TGCAC
Asp700I 1828 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1727 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 815 TGGCCA TGG^CCA
BbeI 2584 GGCGCC GGCGC^C
BcgI 1583 CGAGAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1152 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1152 GCTGAGC GC^T[ACGT]AGC
BsaWI 2236 ACCGGA [AT]^CCGG[AT]
BsmBI 2549 GAGACG CGTCTC(1/5)
Bsp1407I 2855 TGTACA T^GTACA
BspHI 781 TCATGA T^CATGA
BspLU11I 2852 ACATGT A^CATGT
BsrGI 2855 TGTACA T^GTACA
BstAPI 911 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1134 GGTGACC G^GT[ACGT]ACC
BstPI 1134 GGTGACC G^GT[ACGT]ACC
BstUI 2313 CGCG CG^CG
CelII 1152 GCTGAGC GC^T[ACGT]AGC
DraIII 2479 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1727 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1247 CTCTTC CTCTTC(1/4)
Eco47III 1194 AGCGCT AGC^GCT
EcoO65I 1134 GGTGACC G^GT[ACGT]ACC
KasI 2584 GGCGCC G^GCGCC
Ksp632I 1247 CTCTTC CTCTTC(1/4)
KspI 2312 CCGCGG CCGC^GG
MfeI 3236 CAATTG C^AATTG
MluNI 815 TGGCCA TGG^CCA
MscI 815 TGGCCA TGG^CCA
MunI 3236 CAATTG C^AATTG
MvnI 2313 CGCG CG^CG
NarI 2584 GGCGCC GG^CGCC
PaeR7I 1124 CTCGAG C^TCGAG
PmeI 3505 GTTTAAAC GTTT^AAAC
RcaI 781 TCATGA T^CATGA
SacII 2312 CCGCGG CCGC^GG
SalI 1735 GTCGAC G^TCGAC
ScaI 414 AGTACT AGT^ACT
SexAI 3202 ACCTGGT A^CC[AT]GGT
SfoI 2584 GGCGCC GGC^GCC
SgrAI 1859 CACCGGCG C[GA]^CCGG[CT]G
SspBI 2855 TGTACA T^GTACA
StuI 1684 AGGCCT AGG^CCT
XhoI 1124 CTCGAG C^TCGAG
XmnI 1828 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 344 GGTACC G^GTACC
1304 GGTACC
AccI 1735 GTCGAC GT^[AC][GT]AC
3147 GTAGAC
AcyI 1109 GACGCC G[GA]^CG[CT]C
2584 GGCGCC
AflII 2705 CTTAAG C^TTAAG
2884 CTTAAG
Asp718I 344 GGTACC G^GTACC
1304 GGTACC
BbrPI 3051 CACGTG CAC^GTG
3902 CACGTG
BciVI 1595 GGATAC GTATCC(6/5)
378 GTATCC
BfrI 2705 CTTAAG C^TTAAG
2884 CTTAAG
BglI 2310 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2576 GCCTGCTAGGC
BpmI 997 CTCCAG CTGGAG(16/14)
1183 CTGGAG
BsaAI 3051 CACGTG [CT]AC^GT[GA]
3902 CACGTG
BsaHI 1109 GACGCC G[GA]^CG[CT]C
2584 GGCGCC
BsiEI 1796 CGGCCG CG[GA][CT]^CG
2183 CGGCCG
BsrFI 1668 GCCGGT [GA]^CCGG[CT]
1860 ACCGGC
BstXI 645 CCACCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1510 CCAGCAGAGTGG
Cfr10I 1668 GCCGGT [GA]^CCGG[CT]
1860 ACCGGC
EagI 1796 CGGCCG C^GGCCG
2183 CGGCCG
EclXI 1796 CGGCCG C^GGCCG
2183 CGGCCG
Eco52I 1796 CGGCCG C^GGCCG
2183 CGGCCG
EcoRV 368 GATATC GAT^ATC
3565 GATATC
HgaI 1109 GACGC GACGC(5/10)
4 GCGTC
Hin1I 1109 GACGCC G[GA]^CG[CT]C
2584 GGCGCC
HincII 1735 GTCGAC GT[CT]^[GA]AC
3544 GTCAAC
HindII 1735 GTCGAC GT[CT]^[GA]AC
3544 GTCAAC
HindIII 2720 AAGCTT A^AGCTT
2887 AAGCTT
KpnI 344 GGTACC GGTAC^C
1304 GGTACC
NcoI 1139 CCATGG C^CATGG
1534 CCATGG
PmaCI 3051 CACGTG CAC^GTG
3902 CACGTG
PmlI 3051 CACGTG CAC^GTG
3902 CACGTG
PvuII 1642 CAGCTG CAG^CTG
3297 CAGCTG
SacI 1121 GAGCTC GAGCT^C
1186 GAGCTC
SphI 55 GCATGC GCATG^C
2227 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI