##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BclI BfrI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2635 GACGTC GACGT^C
AcyI 2635 GACGTC G[GA]^CG[CT]C
AfaI 798 GTAC GT^AC
Aor51HI 1945 AGCGCT AGC^GCT
AseI 2686 ATTAAT AT^TAAT
AsnI 2686 ATTAAT AT^TAAT
BalI 1176 TGGCCA TGG^CCA
BbsI 3318 GAAGAC GAAGAC(2/6)
BcgI 949 GCAGGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1449 GTATCC GTATCC(6/5)
BlpI 1941 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1941 GCTGAGC GC^T[ACGT]AGC
BpuAI 3318 GAAGAC GAAGAC(2/6)
BsaHI 2635 GACGTC G[GA]^CG[CT]C
BsaWI 3072 TCCGGT [AT]^CCGG[AT]
CelII 1941 GCTGAGC GC^T[ACGT]AGC
DrdI 373 GACTCCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1176 TGGCCA [CT]^GGCC[GA]
Eco47III 1945 AGCGCT AGC^GCT
EcoRI 972 GAATTC G^AATTC
EcoRV 1021 GATATC GAT^ATC
Hin1I 2635 GACGTC G[GA]^CG[CT]C
MluNI 1176 TGGCCA TGG^CCA
MscI 1176 TGGCCA TGG^CCA
PshBI 2686 ATTAAT AT^TAAT
PstI 1210 CTGCAG CTGCA^G
RsaI 798 GTAC GT^AC
SspI 724 AATATT AAT^ATT
TaqI 2678 TCGA T^CGA
TfiI 3003 GAATC G^A[AT]TC
TthHB8I 2678 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 426 GGGCCC GGGCC^C
3214 GGGCCC
AspHI 2981 GAGCTC G[AT]GC[AT]^C
3126 GAGCTC
AspI 1003 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2859 GACCCTGTC
BglII 917 AGATCT A^GATCT
2748 AGATCT
BsiHKAI 2981 GAGCTC G[AT]GC[AT]^C
3126 GAGCTC
BspMI 981 ACCTGC ACCTGC(4/8)
210 GCAGGT
BsrBI 1117 CCGCTC CCGCTC(-3/-3)
1922 CCGCTC
BstAPI 1858 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2893 GCACCTGATGC
PflFI 1003 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2859 GACCCTGTC
PshAI 1495 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1628 GACACCAGTC
PvuII 570 CAGCTG CAG^CTG
2888 CAGCTG
SacI 2981 GAGCTC GAGCT^C
3126 GAGCTC
Tth111I 1003 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2859 GACCCTGTC
XbaI 262 TCTAGA T^CTAGA
823 TCTAGA
XcmI 1179 CCACGGTTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1324 CCACCCTGTCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
AocI ApoI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmaCI XmaI