##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BcnI BglI BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 746 GGTACC G^GTACC
AccI 1090 GTCTAC GT^[AC][GT]AC
AflIII 497 ACATGT A^C[GA][CT]GT
Alw44I 1719 GTGCAC G^TGCAC
AocI 480 CCTGAGG CC^T[ACGT]AGG
ApaLI 1719 GTGCAC G^TGCAC
Asp718I 746 GGTACC G^GTACC
BanI 746 GGTACC G^G[CT][GA]CC
BanII 999 GAGCTC G[GA]GC[CT]^C
BbvCI 780 GCTGAGG CCTCAGC(-5/-2)
BglII 2378 AGATCT A^GATCT
BsaAI 4558 CACGTA [CT]AC^GT[GA]
BsmAI 2949 GAGAC GTCTC(1/5)
BspLU11I 497 ACATGT A^CATGT
BspMI 1220 ACCTGC ACCTGC(4/8)
BstAPI 3054 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2184 CCAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 480 CCTGAGG CC^T[ACGT]AGG
DraII 244 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 4569 GACAATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1300 TGGCCG [CT]^GGCC[GA]
Eco81I 480 CCTGAGG CC^T[ACGT]AGG
EcoNI 2211 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 244 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 4008 ATGCAT ATGCA^T
HapII 1303 CCGG C^CGG
HindIII 4259 AAGCTT A^AGCTT
HpaI 3964 GTTAAC GTT^AAC
HpaII 1303 CCGG C^CGG
KpnI 746 GGTACC GGTAC^C
MfeI 4432 CAATTG C^AATTG
MspA1I 1296 CAGCTG C[AC]G^C[GT]G
MspI 1303 CCGG C^CGG
MunI 4432 CAATTG C^AATTG
NdeI 4132 CATATG CA^TATG
NsiI 4008 ATGCAT ATGCA^T
PpuMI 244 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1296 CAGCTG CAG^CTG
SacI 999 GAGCTC GAGCT^C
SfcI 1985 CTACAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 1110 GCGG CCGC(-3/-1)
1224 GCGG
AlwNI 1683 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2947 CAGAGACTG
AvaII 245 GGACC G^G[AT]CC
1226 GGTCC
BpmI 13 CTGGAG CTGGAG(16/14)
1431 CTGGAG
BsaBI 540 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2447 GATTTACATC
BsgI 583 GTGCAG GTGCAG(16/14)
696 GTGCAG
BsmFI 244 GGGAC GGGAC(10/14)
2250 GGGAC
DsaI 955 CCATGG C^C[GA][CT]GG
1855 CCATGG
EarI 2609 CTCTTC CTCTTC(1/4)
3136 GAAGAG
EcoRV 1182 GATATC GAT^ATC
4891 GATATC
Ksp632I 2609 CTCTTC CTCTTC(1/4)
3136 GAAGAG
MaeII 2263 ACGT A^CGT
4559 ACGT
MamI 540 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2447 GATTTACATC
NcoI 955 CCATGG C^CATGG
1855 CCATGG
NheI 864 GCTAGC G^CTAGC
2361 GCTAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
ApoI AseI AsnI Asp700I AspHI
BbsI BbvI BfaI BfrI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmnI