##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AclI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AviII AvrII
BamHI BbeI BbrPI BglI BlnI
BsiEI BsiWI BsmBI BspDI BspHI
BsrBI BsrFI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
FseI FspI KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I StuI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1966 GACGTC GACGT^C
Acc65I 3011 GGTACC G^GTACC
AccI 347 GTCTAC GT^[AC][GT]AC
AccII 2646 CGCG CG^CG
AccIII 2671 TCCGGA T^CCGGA
AflII 3941 CTTAAG C^TTAAG
AocI 2841 CCTGAGG CC^T[ACGT]AGG
ApaI 4021 GGGCCC GGGCC^C
AseI 2487 ATTAAT AT^TAAT
AsnI 2487 ATTAAT AT^TAAT
Asp700I 227 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3011 GGTACC G^GTACC
AspI 2046 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3011 GGTACC G^G[CT][GA]CC
BbvCI 2619 GCTGAGG CCTCAGC(-5/-2)
BcgI 1495 CGAGGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3941 CTTAAG C^TTAAG
BsaBI 1620 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2671 TCCGGA [AT]^CCGG[AT]
BseAI 2671 TCCGGA T^CCGGA
BsgI 4436 GTGCAG GTGCAG(16/14)
BspEI 2671 TCCGGA T^CCGGA
BstBI 1834 TTCGAA TT^CGAA
BstEII 2985 GGTCACC G^GT[ACGT]ACC
BstPI 2985 GGTCACC G^GT[ACGT]ACC
BstUI 2646 CGCG CG^CG
Bsu36I 2841 CCTGAGG CC^T[ACGT]AGG
Eco81I 2841 CCTGAGG CC^T[ACGT]AGG
EcoNI 1097 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2985 GGTCACC G^GT[ACGT]ACC
EcoT22I 3448 ATGCAT ATGCA^T
FauI 4051 CCCGC CCCGC(4/6)
HaeII 1003 AGCGCC [GA]GCGC^[CT]
KpnI 3011 GGTACC GGTAC^C
MamI 1620 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3903 CAATTG C^AATTG
MroI 2671 TCCGGA T^CCGGA
MslI 1316 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3903 CAATTG C^AATTG
MvnI 2646 CGCG CG^CG
NruI 2645 TCGCGA TCG^CGA
NsiI 3448 ATGCAT ATGCA^T
NspV 1834 TTCGAA TT^CGAA
PflFI 2046 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2487 ATTAAT AT^TAAT
SfuI 1834 TTCGAA TT^CGAA
SmaI 2864 CCCGGG CCC^GGG
SphI 2096 GCATGC GCATG^C
SspI 469 AATATT AAT^ATT
SwaI 4337 ATTTAAAT ATTT^AAAT
Tth111I 2046 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2864 CCCGGG C^CCGGG
XmaI 2864 CCCGGG C^CCGGG
XmnI 227 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1966 GACGTC G[GA]^CG[CT]C
2026 GGCGTC
BbsI 4001 GAAGAC GAAGAC(2/6)
1261 GTCTTC
BciVI 2333 GGATAC GTATCC(6/5)
3547 GTATCC
BclI 2182 TGATCA T^GATCA
2477 TGATCA
BpuAI 4001 GAAGAC GAAGAC(2/6)
1261 GTCTTC
BsaAI 439 TACGTG [CT]AC^GT[GA]
4078 TACGTG
BsaHI 1966 GACGTC G[GA]^CG[CT]C
2026 GGCGTC
Bsp1407I 3414 TGTACA T^GTACA
4172 TGTACA
BspLU11I 792 ACATGT A^CATGT
3142 ACATGT
BsrGI 3414 TGTACA T^GTACA
4172 TGTACA
BssSI 286 CACGAG CACGAG(-5/-1)
3107 CACGAG
DraIII 547 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
1314 CACACAGTG
EcoRV 603 GATATC GAT^ATC
4110 GATATC
FbaI 2182 TGATCA T^GATCA
2477 TGATCA
HgaI 2027 GCGTC GACGC(5/10)
3870 GCGTC
Hin1I 1966 GACGTC G[GA]^CG[CT]C
2026 GGCGTC
HincII 1254 GTTAAC GT[CT]^[GA]AC
4528 GTTAAC
HindII 1254 GTTAAC GT[CT]^[GA]AC
4528 GTTAAC
HpaI 1254 GTTAAC GTT^AAC
4528 GTTAAC
PaeR7I 1806 CTCGAG C^TCGAG
3016 CTCGAG
SacI 201 GAGCTC GAGCT^C
2205 GAGCTC
SspBI 3414 TGTACA T^GTACA
4172 TGTACA
XhoI 1806 CTCGAG C^TCGAG
3016 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII