##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AviII
BbeI BbrPI BbsI BciVI BclI
BfrI BglI BglII BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiWI BspDI BspEI BsrDI BssHII
BssSI BstUI CelII CfoI ClaI
CpoI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2879 GGTACC G^GTACC
AclI 1470 AACGTT AA^CGTT
AflIII 2203 ACATGT A^C[GA][CT]GT
Asp718I 2879 GGTACC G^GTACC
AvrII 1862 CCTAGG C^CTAGG
BamHI 1050 GGATCC G^GATCC
BlnI 1862 CCTAGG C^CTAGG
BsiEI 3698 CGGTCG CG[GA][CT]^CG
BsmBI 1714 GAGACG CGTCTC(1/5)
BsmFI 874 GGGAC GGGAC(10/14)
Bsp1407I 2016 TGTACA T^GTACA
BspHI 633 TCATGA T^CATGA
BspLU11I 2203 ACATGT A^CATGT
BspMI 1029 ACCTGC ACCTGC(4/8)
BsrBI 3566 GAGCGG CCGCTC(-3/-3)
BsrGI 2016 TGTACA T^GTACA
BstBI 3049 TTCGAA TT^CGAA
BstEII 479 GGTGACC G^GT[ACGT]ACC
BstPI 479 GGTGACC G^GT[ACGT]ACC
EcoNI 2127 CCTTTATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 479 GGTGACC G^GT[ACGT]ACC
EcoT22I 2751 ATGCAT ATGCA^T
HgaI 350 GACGC GACGC(5/10)
HindIII 1755 AAGCTT A^AGCTT
KpnI 2879 GGTACC GGTAC^C
MfeI 3452 CAATTG C^AATTG
MunI 3452 CAATTG C^AATTG
NdeI 3430 CATATG CA^TATG
NsiI 2751 ATGCAT ATGCA^T
NspV 3049 TTCGAA TT^CGAA
PmeI 3536 GTTTAAAC GTTT^AAAC
Psp1406I 1470 AACGTT AA^CGTT
RcaI 633 TCATGA T^CATGA
SapI 377 GAAGAGC GCTCTTC(1/4)
ScaI 2077 AGTACT AGT^ACT
SfuI 3049 TTCGAA TT^CGAA
SmaI 180 CCCGGG CCC^GGG
SspBI 2016 TGTACA T^GTACA
XmaCI 180 CCCGGG C^CCGGG
XmaI 180 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1348 GTATAC GT^[AC][GT]AC
2580 GTATAC
AciI 2110 CCGC CCGC(-3/-1)
3568 GCGG
Alw44I 228 GTGCAC G^TGCAC
3413 GTGCAC
AlwNI 2187 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
2826 CAGTAACTG
AocI 3383 CCTTAGG CC^T[ACGT]AGG
3623 CCTGAGG
ApaLI 228 GTGCAC G^TGCAC
3413 GTGCAC
AvaI 117 CTCGGG C^[CT]CG[GA]G
180 CCCGGG
BbvCI 773 CCTCAGC CCTCAGC(-5/-2)
3740 GCTGAGG
BsaI 2554 GAGACC GGTCTC(1/5)
3640 GAGACC
BsaWI 232 ACCGGA [AT]^CCGG[AT]
741 ACCGGA
BseRI 38 GAGGAG GAGGAG(10/8)
497 GAGGAG
BsmI 430 GCATTC GAATGC(1/-1)
1926 GCATTC
BsoBI 117 CTCGGG C^[CT]CG[GA]G
180 CCCGGG
BsrFI 1039 GCCGGT [GA]^CCGG[CT]
3696 GCCGGT
Bst1107I 1348 GTATAC GTA^TAC
2580 GTATAC
BstXI 336 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2317 CCAAAGAGGTGG
BstZ17I 1348 GTATAC GTA^TAC
2580 GTATAC
Bsu36I 3383 CCTTAGG CC^T[ACGT]AGG
3623 CCTGAGG
Cfr10I 1039 GCCGGT [GA]^CCGG[CT]
3696 GCCGGT
DraIII 1672 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
2697 CACAAAGTG
Eco81I 3383 CCTTAGG CC^T[ACGT]AGG
3623 CCTGAGG
EcoRV 857 GATATC GAT^ATC
3311 GATATC
EcoT14I 624 CCAAGG C^C[AT][AT]GG
1862 CCTAGG
MaeII 1471 ACGT A^CGT
2104 ACGT
MslI 1163 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2334 CATCAGAATG
PstI 407 CTGCAG CTGCA^G
1835 CTGCAG
SacI 637 GAGCTC GAGCT^C
785 GAGCTC
StyI 624 CCAAGG C^C[AT][AT]GG
1862 CCTAGG
XcmI 403 CCAGCTGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2696 CCACAAAGTGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbvI BcgI BcnI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI