##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AviII AvrII
BbeI BbrPI BglII BlnI BlpI
Bpu1102I BsaHI BsiWI BsmBI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I FseI FspI
Hin1I KasI KpnI KspI MluI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 2037 TCCGGA T^CCGGA
AflII 465 CTTAAG C^TTAAG
AhdI 3329 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3329 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 175 GGATCC G^GATCC
BanI 1714 GGTGCC G^G[CT][GA]CC
BanII 4059 GAGCTC G[GA]GC[CT]^C
BbsI 640 GAAGAC GAAGAC(2/6)
BcgI 1271 GCAAAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 465 CTTAAG C^TTAAG
BglI 2397 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 640 GAAGAC GAAGAC(2/6)
BsaAI 1255 TACGTG [CT]AC^GT[GA]
BsaI 24 GAGACC GGTCTC(1/5)
BseAI 2037 TCCGGA T^CCGGA
BseRI 1861 GAGGAG GAGGAG(10/8)
BsgI 2784 GTGCAG GTGCAG(16/14)
BsiEI 1021 CGACCG CG[GA][CT]^CG
BspDI 931 ATCGAT AT^CGAT
BspEI 2037 TCCGGA T^CCGGA
BspMI 1592 GCAGGT ACCTGC(4/8)
BsrFI 350 ACCGGC [GA]^CCGG[CT]
BstBI 471 TTCGAA TT^CGAA
BstXI 1448 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1445 GCGC GCG^C
Cfr10I 350 ACCGGC [GA]^CCGG[CT]
ClaI 931 ATCGAT AT^CGAT
DraIII 3212 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 760 CCATGG C^C[GA][CT]GG
Eam1105I 3329 GACTAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2890 GAATTC G^AATTC
EcoT22I 3939 ATGCAT ATGCA^T
HaeII 1444 AGCGCC [GA]GCGC^[CT]
HhaI 1445 GCGC GCG^C
HinP1I 1445 GCGC G^CGC
MfeI 2637 CAATTG C^AATTG
MroI 2037 TCCGGA T^CCGGA
MunI 2637 CAATTG C^AATTG
NcoI 760 CCATGG C^CATGG
NsiI 3939 ATGCAT ATGCA^T
NspV 471 TTCGAA TT^CGAA
PaeR7I 550 CTCGAG C^TCGAG
PvuII 1900 CAGCTG CAG^CTG
SacI 4059 GAGCTC GAGCT^C
SfuI 471 TTCGAA TT^CGAA
SphI 3937 GCATGC GCATG^C
SwaI 3608 ATTTAAAT ATTT^AAAT
XbaI 478 TCTAGA T^CTAGA
XcmI 751 CCAGGCGTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 550 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 131 CCTCAGG CC^T[ACGT]AGG
170 CCTCAGG
Asp700I 2502 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3220 GAAATATTTC
AspHI 2861 GAGCAC G[AT]GC[AT]^C
4059 GAGCTC
AspI 1563 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1938 GACCCCGTC
BalI 1457 TGGCCA TGG^CCA
1995 TGGCCA
BbvCI 2215 CCTCAGC CCTCAGC(-5/-2)
424 GCTGAGG
BciVI 1223 GGATAC GTATCC(6/5)
1315 GGATAC
BmyI 2861 GAGCAC G[AGT]GC[ACT]^C
4059 GAGCTC
BpmI 749 CTCCAG CTGGAG(16/14)
1890 CTGGAG
BsaBI 775 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2263 GATGGATATC
BsiHKAI 2861 GAGCAC G[AT]GC[AT]^C
4059 GAGCTC
Bsp1286I 2861 GAGCAC G[AGT]GC[ACT]^C
4059 GAGCTC
Bsp1407I 2466 TGTACA T^GTACA
3152 TGTACA
BsrGI 2466 TGTACA T^GTACA
3152 TGTACA
Bsu36I 131 CCTCAGG CC^T[ACGT]AGG
170 CCTCAGG
EaeI 1457 TGGCCA [CT]^GGCC[GA]
1995 TGGCCA
Eco81I 131 CCTCAGG CC^T[ACGT]AGG
170 CCTCAGG
EcoRV 895 GATATC GAT^ATC
2267 GATATC
HgaI 500 GACGC GACGC(5/10)
342 GCGTC
HindIII 1142 AAGCTT A^AGCTT
1179 AAGCTT
HpaI 2713 GTTAAC GTT^AAC
4083 GTTAAC
MamI 775 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2263 GATGGATATC
MluNI 1457 TGGCCA TGG^CCA
1995 TGGCCA
MscI 1457 TGGCCA TGG^CCA
1995 TGGCCA
MspA1I 307 CAGCGG C[AC]G^C[GT]G
1900 CAGCTG
NdeI 2470 CATATG CA^TATG
3156 CATATG
PflFI 1563 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1938 GACCCCGTC
PstI 1883 CTGCAG CTGCA^G
2422 CTGCAG
SmaI 188 CCCGGG CCC^GGG
2207 CCCGGG
SspBI 2466 TGTACA T^GTACA
3152 TGTACA
Tth111I 1563 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1938 GACCCCGTC
XmaCI 188 CCCGGG C^CCGGG
2207 CCCGGG
XmaI 188 CCCGGG C^CCGGG
2207 CCCGGG
XmnI 2502 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3220 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AvaI AvaII BbvI BclI BcnI
BfaI BsaJI BsaWI BsiYI BslI
BsmAI BsmFI BsmI BsoBI BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII