##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AvrII
BamHI BbeI BbrPI BbvCI BcgI
BglII BlnI BseAI BsmBI BspDI
BspEI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI DrdI Eco47III
Eco81I EcoO65I EcoRV FseI HindIII
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PmaCI
PmeI PmlI PshAI PshBI PvuI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1068 GACGTC GACGT^C
AcyI 1068 GACGTC G[GA]^CG[CT]C
AflII 4111 CTTAAG C^TTAAG
AflIII 66 ACATGT A^C[GA][CT]GT
AgeI 2992 ACCGGT A^CCGGT
AhdI 3515 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3211 GTGCAC G^TGCAC
ApaLI 3211 GTGCAC G^TGCAC
Asp700I 1324 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3515 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3946 TGCGCA TGC^GCA
BfrI 4111 CTTAAG C^TTAAG
BglI 2815 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2785 TACGTG [CT]AC^GT[GA]
BsaHI 1068 GACGTC G[GA]^CG[CT]C
BsaI 1835 GAGACC GGTCTC(1/5)
BsiEI 182 CGGCCG CG[GA][CT]^CG
BsiWI 3304 CGTACG C^GTACG
Bsp1407I 3385 TGTACA T^GTACA
BspHI 437 TCATGA T^CATGA
BspLU11I 66 ACATGT A^CATGT
BsrGI 3385 TGTACA T^GTACA
BssHII 1084 GCGCGC G^CGCGC
BssSI 3075 CACGAG CACGAG(-5/-1)
CpoI 871 CGGACCG CG^G[AT]CCG
DraIII 1552 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 182 CGGCCG C^GGCCG
Eam1105I 3515 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 182 CGGCCG C^GGCCG
Eco52I 182 CGGCCG C^GGCCG
EcoNI 3635 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 638 ATGCAT ATGCA^T
FspI 3946 TGCGCA TGC^GCA
HaeII 2823 GGCGCT [GA]GCGC^[CT]
Hin1I 1068 GACGTC G[GA]^CG[CT]C
HpaI 4068 GTTAAC GTT^AAC
NdeI 746 CATATG CA^TATG
NsiI 638 ATGCAT ATGCA^T
PinAI 2992 ACCGGT A^CCGGT
RcaI 437 TCATGA T^CATGA
RsrII 871 CGGACCG CG^G[AT]CCG
SacI 2368 GAGCTC GAGCT^C
SapI 4150 GCTCTTC GCTCTTC(1/4)
SmaI 2970 CCCGGG CCC^GGG
SphI 1715 GCATGC GCATG^C
SspBI 3385 TGTACA T^GTACA
XbaI 544 TCTAGA T^CTAGA
XmaCI 2970 CCCGGG C^CCGGG
XmaI 2970 CCCGGG C^CCGGG
XmnI 1324 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 559 GTAGAC GT^[AC][GT]AC
2308 GTCTAC
AclI 766 AACGTT AA^CGTT
1188 AACGTT
AspHI 2368 GAGCTC G[AT]GC[AT]^C
3211 GTGCAC
AspI 829 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3285 GACAAGGTC
BanI 1867 GGTGCC G^G[CT][GA]CC
3438 GGTGCC
BclI 434 TGATCA T^GATCA
2322 TGATCA
BsaBI 429 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1155 GATTCGTATC
BsgI 2658 CTGCAC GTGCAG(16/14)
2350 GTGCAG
BsiHKAI 2368 GAGCTC G[AT]GC[AT]^C
3211 GTGCAC
BsmI 2105 GAATGC GAATGC(1/-1)
2031 GCATTC
BspMI 2705 ACCTGC ACCTGC(4/8)
3835 ACCTGC
BsrBI 1435 CCGCTC CCGCTC(-3/-3)
3443 CCGCTC
BsrFI 1787 GCCGGT [GA]^CCGG[CT]
2992 ACCGGT
BstAPI 772 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1225 GCAGTCTGTGC
BstYI 121 AGATCC [GA]^GATC[CT]
1039 GGATCT
Cfr10I 1787 GCCGGT [GA]^CCGG[CT]
2992 ACCGGT
DraI 3858 TTTAAA TTT^AAA
3874 TTTAAA
DraII 2964 GGGTCCC [GA]G^G[ACGT]CC[CT]
3521 GGGTCCT
EcoO109I 2964 GGGTCCC [GA]G^G[ACGT]CC[CT]
3521 GGGTCCT
EcoRI 721 GAATTC G^AATTC
3895 GAATTC
FbaI 434 TGATCA T^GATCA
2322 TGATCA
KspI 108 CCGCGG CCGC^GG
2613 CCGCGG
MamI 429 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1155 GATTCGTATC
MflI 121 AGATCC [GA]^GATC[CT]
1039 GGATCT
PaeR7I 3249 CTCGAG C^TCGAG
3472 CTCGAG
PflFI 829 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3285 GACAAGGTC
PflMI 1840 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4195 CCATTAACTGG
PpuMI 2964 GGGTCCC [GA]G^G[AT]CC[CT]
3521 GGGTCCT
Psp1406I 766 AACGTT AA^CGTT
1188 AACGTT
SacII 108 CCGCGG CCGC^GG
2613 CCGCGG
StuI 2954 AGGCCT AGG^CCT
3495 AGGCCT
Tth111I 829 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3285 GACAAGGTC
Van91I 1840 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4195 CCATTAACTGG
XcmI 1511 CCAAAATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1840 CCATATTGTGGATGG
XhoI 3249 CTCGAG C^TCGAG
3472 CTCGAG
XhoII 121 AGATCC [GA]^GATC[CT]
1039 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BalI BanII BbsI BbvI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I