##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HpaII       KasI        
KpnI        KspI        MflI        MluI        MroI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NlaIV       NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AclI          148  AACGTT               AA^CGTT
AcyI          695  GGCGTC               G[GA]^CG[CT]C
AflII        2580  CTTAAG               C^TTAAG
AspHI         911  GAGCAC               G[AT]GC[AT]^C
BfrI         2580  CTTAAG               C^TTAAG
BmyI          911  GAGCAC               G[AGT]GC[ACT]^C
BsaHI         695  GGCGTC               G[GA]^CG[CT]C
BsiHKAI       911  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      911  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I      680  TGTACA               T^GTACA
BspMI        1523  ACCTGC               ACCTGC(4/8)
BsrGI         680  TGTACA               T^GTACA
Bst1107I     2171  GTATAC               GTA^TAC
BstXI        1021  CCACAGGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2171  GTATAC               GTA^TAC
CfoI          144  GCGC                 GCG^C
EcoNI        2307  CCTTTATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        3249  GATATC               GAT^ATC
EcoT22I      2071  ATGCAT               ATGCA^T
HhaI          144  GCGC                 GCG^C
Hin1I         695  GGCGTC               G[GA]^CG[CT]C
HinP1I        144  GCGC                 G^CGC
MfeI          209  CAATTG               C^AATTG
MunI          209  CAATTG               C^AATTG
NdeI         1421  CATATG               CA^TATG
NsiI         2071  ATGCAT               ATGCA^T
Psp1406I      148  AACGTT               AA^CGTT
SexAI        2626  ACCTGGT              A^CC[AT]GGT
SpeI         2175  ACTAGT               A^CTAGT
SphI         3059  GCATGC               GCATG^C
SspBI         680  TGTACA               T^GTACA
StuI         2274  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI         1245  GTAGAC               GT^[AC][GT]AC
             2171  GTATAC              
AseI         1321  ATTAAT               AT^TAAT
             1950  ATTAAT              
AsnI         1321  ATTAAT               AT^TAAT
             1950  ATTAAT              
AvaII         448  GGACC                G^G[AT]CC
              724  GGTCC               
BalI          117  TGGCCA               TGG^CCA
             1695  TGGCCA              
BpmI          422  CTCCAG               CTGGAG(16/14)
              901  CTCCAG              
BsaBI         835  GATAACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3706  GATGGTAATC          
BsaI          787  GAGACC               GGTCTC(1/5)
              898  GGTCTC              
BspHI          84  TCATGA               T^CATGA
             2374  TCATGA              
EaeI          117  TGGCCA               [CT]^GGCC[GA]
             1695  TGGCCA              
EarI          283  GAAGAG               CTCTTC(1/4)
              520  GAAGAG              
EcoRI        1595  GAATTC               G^AATTC
             3528  GAATTC              
HgaI          648  GCGTC                GACGC(5/10)
              696  GCGTC               
HindIII      2583  AAGCTT               A^AGCTT
             2986  AAGCTT              
Ksp632I       283  GAAGAG               CTCTTC(1/4)
              520  GAAGAG              
MamI          835  GATAACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3706  GATGGTAATC          
MluNI         117  TGGCCA               TGG^CCA
             1695  TGGCCA              
MscI          117  TGGCCA               TGG^CCA
             1695  TGGCCA              
MspA1I        256  CAGCTG               C[AC]G^C[GT]G
              904  CAGCTG              
MwoI         2236  GCTGATCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             2922  GCTTATTTAGC         
PshBI        1321  ATTAAT               AT^TAAT
             1950  ATTAAT              
PvuII         256  CAGCTG               CAG^CTG
              904  CAGCTG              
RcaI           84  TCATGA               T^CATGA
             2374  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
ApoI        Asp700I     BbsI        BbvI        BfaI        
BpuAI       BsaJI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        BspLU11I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HincII      
HindII      HinfI       HpaI        HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        NdeII       
NlaIII      NspI        PleI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XbaI        
XmnI