################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FauI FbaI FseI FspI HaeII HapII HpaII KasI KpnI KspI MflI MluI MroI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AclI 148 AACGTT AA^CGTT AcyI 695 GGCGTC G[GA]^CG[CT]C AflII 2580 CTTAAG C^TTAAG AspHI 911 GAGCAC G[AT]GC[AT]^C BfrI 2580 CTTAAG C^TTAAG BmyI 911 GAGCAC G[AGT]GC[ACT]^C BsaHI 695 GGCGTC G[GA]^CG[CT]C BsiHKAI 911 GAGCAC G[AT]GC[AT]^C Bsp1286I 911 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 680 TGTACA T^GTACA BspMI 1523 ACCTGC ACCTGC(4/8) BsrGI 680 TGTACA T^GTACA Bst1107I 2171 GTATAC GTA^TAC BstXI 1021 CCACAGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2171 GTATAC GTA^TAC CfoI 144 GCGC GCG^C EcoNI 2307 CCTTTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3249 GATATC GAT^ATC EcoT22I 2071 ATGCAT ATGCA^T HhaI 144 GCGC GCG^C Hin1I 695 GGCGTC G[GA]^CG[CT]C HinP1I 144 GCGC G^CGC MfeI 209 CAATTG C^AATTG MunI 209 CAATTG C^AATTG NdeI 1421 CATATG CA^TATG NsiI 2071 ATGCAT ATGCA^T Psp1406I 148 AACGTT AA^CGTT SexAI 2626 ACCTGGT A^CC[AT]GGT SpeI 2175 ACTAGT A^CTAGT SphI 3059 GCATGC GCATG^C SspBI 680 TGTACA T^GTACA StuI 2274 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1245 GTAGAC GT^[AC][GT]AC 2171 GTATAC AseI 1321 ATTAAT AT^TAAT 1950 ATTAAT AsnI 1321 ATTAAT AT^TAAT 1950 ATTAAT AvaII 448 GGACC G^G[AT]CC 724 GGTCC BalI 117 TGGCCA TGG^CCA 1695 TGGCCA BpmI 422 CTCCAG CTGGAG(16/14) 901 CTCCAG BsaBI 835 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3706 GATGGTAATC BsaI 787 GAGACC GGTCTC(1/5) 898 GGTCTC BspHI 84 TCATGA T^CATGA 2374 TCATGA EaeI 117 TGGCCA [CT]^GGCC[GA] 1695 TGGCCA EarI 283 GAAGAG CTCTTC(1/4) 520 GAAGAG EcoRI 1595 GAATTC G^AATTC 3528 GAATTC HgaI 648 GCGTC GACGC(5/10) 696 GCGTC HindIII 2583 AAGCTT A^AGCTT 2986 AAGCTT Ksp632I 283 GAAGAG CTCTTC(1/4) 520 GAAGAG MamI 835 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3706 GATGGTAATC MluNI 117 TGGCCA TGG^CCA 1695 TGGCCA MscI 117 TGGCCA TGG^CCA 1695 TGGCCA MspA1I 256 CAGCTG C[AC]G^C[GT]G 904 CAGCTG MwoI 2236 GCTGATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 2922 GCTTATTTAGC PshBI 1321 ATTAAT AT^TAAT 1950 ATTAAT PvuII 256 CAGCTG CAG^CTG 904 CAGCTG RcaI 84 TCATGA T^CATGA 2374 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI Asp700I BbsI BbvI BfaI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsmI BspLU11I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HpaI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NspI PleI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XmnI