##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccI AccII AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
AspI AvrII BbrPI BclI BfrI
BlnI BsaAI BsaBI BsiEI BsiWI
BsmI BspDI BspHI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI KspI
MamI MfeI MluI MunI MvnI
NaeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SfiI SgrAI SnaBI SpeI SphI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2295 GACGTC GACGT^C
AccIII 2638 TCCGGA T^CCGGA
AhdI 633 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1630 AGCGCT AGC^GCT
ApaI 2607 GGGCCC GGGCC^C
Asp700I 752 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 633 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2263 TGCGCA TGC^GCA
BamHI 3278 GGATCC G^GATCC
BbeI 2275 GGCGCC GGCGC^C
BciVI 3537 GTATCC GTATCC(6/5)
BglI 1645 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3178 AGATCT A^GATCT
BseAI 2638 TCCGGA T^CCGGA
Bsp1407I 1789 TGTACA T^GTACA
BspEI 2638 TCCGGA T^CCGGA
BsrFI 2587 ACCGGC [GA]^CCGG[CT]
BsrGI 1789 TGTACA T^GTACA
BstBI 2526 TTCGAA TT^CGAA
Cfr10I 2587 ACCGGC [GA]^CCGG[CT]
Eam1105I 633 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1630 AGCGCT AGC^GCT
FspI 2263 TGCGCA TGC^GCA
HindIII 293 AAGCTT A^AGCTT
KasI 2275 GGCGCC G^GCGCC
MroI 2638 TCCGGA T^CCGGA
NarI 2275 GGCGCC GG^CGCC
NcoI 877 CCATGG C^CATGG
NdeI 3347 CATATG CA^TATG
NheI 3460 GCTAGC G^CTAGC
NspV 2526 TTCGAA TT^CGAA
PshAI 3041 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 3234 AGTACT AGT^ACT
SexAI 2107 ACCTGGT A^CC[AT]GGT
SfoI 2275 GGCGCC GGC^GCC
SfuI 2526 TTCGAA TT^CGAA
SspBI 1789 TGTACA T^GTACA
SspI 660 AATATT AAT^ATT
Tsp509I 746 AATT ^AATT
XbaI 2631 TCTAGA T^CTAGA
XcmI 3661 CCACCTGCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 752 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 2488 GGTACC G^GTACC
3362 GGTACC
AflIII 103 ACATGT A^C[GA][CT]GT
2073 ACATGT
Alw44I 1711 GTGCAC G^TGCAC
2373 GTGCAC
ApaLI 1711 GTGCAC G^TGCAC
2373 GTGCAC
Asp718I 2488 GGTACC G^GTACC
3362 GGTACC
BalI 1117 TGGCCA TGG^CCA
2342 TGGCCA
BcgI 2165 CGAGTCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
718 GCATCTTGATCG
BsaWI 964 TCCGGT [AT]^CCGG[AT]
2638 TCCGGA
BsmBI 1574 CGTCTC CGTCTC(1/5)
2297 CGTCTC
BspLU11I 103 ACATGT A^CATGT
2073 ACATGT
BstAPI 31 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1159 GCAACACATGC
BstXI 574 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
919 CCAGCTGCCTGG
DsaI 877 CCATGG C^C[GA][CT]GG
2339 CCGTGG
HgaI 1346 GACGC GACGC(5/10)
1203 GCGTC
HpaI 2112 GTTAAC GTT^AAC
3100 GTTAAC
KpnI 2488 GGTACC GGTAC^C
3362 GGTACC
MluNI 1117 TGGCCA TGG^CCA
2342 TGGCCA
MscI 1117 TGGCCA TGG^CCA
2342 TGGCCA
SapI 1082 GAAGAGC GCTCTTC(1/4)
2626 GCTCTTC
SmlI 807 CTCAAG C^T[CT][GA]AG
1455 CTCAAG
TfiI 2529 GAATC G^A[AT]TC
3018 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmaI Sse8387I
StuI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI