##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AscI AviII AvrII BbeI BbrPI
BciVI BclI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraIII
DrdI EcoNI EcoO65I EcoRV FbaI
FspI HpaI KasI MamI MluI
MroI NarI NcoI NdeI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2412 GGTACC G^GTACC
AhdI 607 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2846 GTGCAC G^TGCAC
AocI 1422 CCTCAGG CC^T[ACGT]AGG
ApaLI 2846 GTGCAC G^TGCAC
Asp700I 1085 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2412 GGTACC G^GTACC
AspEI 607 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2721 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2905 TGGCCA TGG^CCA
BbvCI 2814 CCTCAGC CCTCAGC(-5/-2)
BglII 1645 AGATCT A^GATCT
BsaWI 164 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3808 TGTACA T^GTACA
BspLU11I 3025 ACATGT A^CATGT
BsrBI 602 GAGCGG CCGCTC(-3/-3)
BsrGI 3808 TGTACA T^GTACA
BstBI 1364 TTCGAA TT^CGAA
Bsu36I 1422 CCTCAGG CC^T[ACGT]AGG
Eam1105I 607 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 905 GAAGAG CTCTTC(1/4)
Eco81I 1422 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3965 ATGCAT ATGCA^T
FseI 442 GGCCGGCC GGCCGG^CC
HincII 4043 GTCAAC GT[CT]^[GA]AC
HindII 4043 GTCAAC GT[CT]^[GA]AC
KpnI 2412 GGTACC GGTAC^C
Ksp632I 905 GAAGAG CTCTTC(1/4)
KspI 665 CCGCGG CCGC^GG
MluNI 2905 TGGCCA TGG^CCA
MscI 2905 TGGCCA TGG^CCA
NaeI 443 GCCGGC GCC^GGC
NgoMIV 443 GCCGGC G^CCGGC
NheI 1541 GCTAGC G^CTAGC
NsiI 3965 ATGCAT ATGCA^T
NspV 1364 TTCGAA TT^CGAA
PflFI 2721 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3820 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3088 AGGACCC [GA]G^G[AT]CC[CT]
SacII 665 CCGCGG CCGC^GG
SapI 905 GAAGAGC GCTCTTC(1/4)
SfuI 1364 TTCGAA TT^CGAA
SmaI 671 CCCGGG CCC^GGG
SphI 2010 GCATGC GCATG^C
SspBI 3808 TGTACA T^GTACA
Tth111I 2721 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3820 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3709 CCAAAAAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
XmnI 1085 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 607 GACGCC G[GA]^CG[CT]C
1009 GGCGTC
AflII 2522 CTTAAG C^TTAAG
3621 CTTAAG
AflIII 2357 ACACGT A^C[GA][CT]GT
3025 ACATGT
AlwNI 1122 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1352 CAGTTTCTG
Aor51HI 1451 AGCGCT AGC^GCT
3771 AGCGCT
ApaI 247 GGGCCC GGGCC^C
2544 GGGCCC
AseI 2552 ATTAAT AT^TAAT
3686 ATTAAT
AsnI 2552 ATTAAT AT^TAAT
3686 ATTAAT
AspHI 2846 GTGCAC G[AT]GC[AT]^C
3958 GTGCTC
BamHI 1860 GGATCC G^GATCC
3678 GGATCC
BbsI 177 GAAGAC GAAGAC(2/6)
956 GAAGAC
BcgI 2803 CGATTCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3947 GCATGTAGTTCG
BfrI 2522 CTTAAG C^TTAAG
3621 CTTAAG
BglI 661 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
713 GCCACGGGGGC
BpuAI 177 GAAGAC GAAGAC(2/6)
956 GAAGAC
BsaHI 607 GACGCC G[GA]^CG[CT]C
1009 GGCGTC
BsaI 2915 GGTCTC GGTCTC(1/5)
3442 GGTCTC
BsiEI 441 CGGCCG CG[GA][CT]^CG
588 CGGCCG
BsiHKAI 2846 GTGCAC G[AT]GC[AT]^C
3958 GTGCTC
BspMI 264 ACCTGC ACCTGC(4/8)
428 GCAGGT
BsrFI 443 GCCGGC [GA]^CCGG[CT]
625 GCCGGT
Cfr10I 443 GCCGGC [GA]^CCGG[CT]
625 GCCGGT
DsaI 665 CCGCGG C^C[GA][CT]GG
714 CCACGG
EagI 441 CGGCCG C^GGCCG
588 CGGCCG
EclXI 441 CGGCCG C^GGCCG
588 CGGCCG
Eco47III 1451 AGCGCT AGC^GCT
3771 AGCGCT
Eco52I 441 CGGCCG C^GGCCG
588 CGGCCG
HaeII 1451 AGCGCT [GA]GCGC^[CT]
3771 AGCGCT
Hin1I 607 GACGCC G[GA]^CG[CT]C
1009 GGCGTC
HindIII 929 AAGCTT A^AGCTT
1088 AAGCTT
MfeI 2271 CAATTG C^AATTG
3334 CAATTG
MunI 2271 CAATTG C^AATTG
3334 CAATTG
PshBI 2552 ATTAAT AT^TAAT
3686 ATTAAT
PvuII 66 CAGCTG CAG^CTG
1578 CAGCTG
ScaI 2390 AGTACT AGT^ACT
3761 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII