##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AspEI AspHI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcnI BglI BlnI BsaBI
BsaHI BsgI BsiHKAI BsiWI BsoBI
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Cfr10I
CpoI DraIII DrdI DsaI Eam1105I
Eco47III EcoO65I EcoRV FauI FseI
FspI HgaI Hin1I KasI KspI
MamI MluI MluNI MscI MspA1I
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3203 GGTACC G^GTACC
AccIII 3845 TCCGGA T^CCGGA
AciI 834 CCGC CCGC(-3/-1)
AflII 2739 CTTAAG C^TTAAG
AlwNI 3253 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3123 CCTCAGG CC^T[ACGT]AGG
ApaI 642 GGGCCC GGGCC^C
Asp718I 3203 GGTACC G^GTACC
AvaII 161 GGTCC G^G[AT]CC
BanI 3203 GGTACC G^G[CT][GA]CC
BcgI 133 GCAATGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3586 GTATCC GTATCC(6/5)
BfrI 2739 CTTAAG C^TTAAG
BglII 1635 AGATCT A^GATCT
BlpI 607 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 607 GCTCAGC GC^T[ACGT]AGC
BsaAI 1243 TACGTG [CT]AC^GT[GA]
BsaI 2398 GGTCTC GGTCTC(1/5)
BseAI 3845 TCCGGA T^CCGGA
BsiEI 831 CGGCCG CG[GA][CT]^CG
BsmBI 1229 CGTCTC CGTCTC(1/5)
BsmFI 3255 GGGAC GGGAC(10/14)
BsmI 1428 GAATGC GAATGC(1/-1)
BspDI 1012 ATCGAT AT^CGAT
BspEI 3845 TCCGGA T^CCGGA
BsrDI 133 GCAATG GCAATG(2/0)
BstAPI 499 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 141 TTCGAA TT^CGAA
BstXI 3395 CCATAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1635 AGATCT [GA]^GATC[CT]
Bsu36I 3123 CCTCAGG CC^T[ACGT]AGG
CelII 607 GCTCAGC GC^T[ACGT]AGC
CfoI 1380 GCGC GCG^C
ClaI 1012 ATCGAT AT^CGAT
EaeI 831 CGGCCG [CT]^GGCC[GA]
EagI 831 CGGCCG C^GGCCG
EclXI 831 CGGCCG C^GGCCG
Eco52I 831 CGGCCG C^GGCCG
Eco81I 3123 CCTCAGG CC^T[ACGT]AGG
EcoNI 1500 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1171 GAATTC G^AATTC
HaeII 1379 GGCGCT [GA]GCGC^[CT]
HhaI 1380 GCGC GCG^C
HinP1I 1380 GCGC G^CGC
HincII 303 GTTAAC GT[CT]^[GA]AC
HindII 303 GTTAAC GT[CT]^[GA]AC
HpaI 303 GTTAAC GTT^AAC
KpnI 3203 GGTACC GGTAC^C
MfeI 2093 CAATTG C^AATTG
MflI 1635 AGATCT [GA]^GATC[CT]
MroI 3845 TCCGGA T^CCGGA
MunI 2093 CAATTG C^AATTG
NspV 141 TTCGAA TT^CGAA
SfuI 141 TTCGAA TT^CGAA
Tsp45I 3555 GTGAC ^GT[GC]AC
XbaI 3458 TCTAGA T^CTAGA
XcmI 2429 CCAAAATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1635 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 1437 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1994 GACTTTGTC
BanII 642 GGGCCC G[GA]GC[CT]^C
887 GGGCTC
BbsI 799 GAAGAC GAAGAC(2/6)
3645 GTCTTC
BclI 276 TGATCA T^GATCA
3507 TGATCA
BpmI 890 CTCCAG CTGGAG(16/14)
1031 CTGGAG
BpuAI 799 GAAGAC GAAGAC(2/6)
3645 GTCTTC
BsaWI 1516 ACCGGA [AT]^CCGG[AT]
3845 TCCGGA
BseRI 3480 CTCCTC GAGGAG(10/8)
3266 GAGGAG
BsiYI 976 CCCAACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1500 CCTCTTTGAGG
BslI 976 CCCAACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1500 CCTCTTTGAGG
Bsp1407I 842 TGTACA T^GTACA
2947 TGTACA
BspMI 2 GCAGGT ACCTGC(4/8)
1131 GCAGGT
BsrGI 842 TGTACA T^GTACA
2947 TGTACA
EcoT14I 2564 CCAAGG C^C[AT][AT]GG
3549 CCTTGG
EcoT22I 962 ATGCAT ATGCA^T
3695 ATGCAT
FbaI 276 TGATCA T^GATCA
3507 TGATCA
HapII 1517 CCGG C^CGG
3846 CCGG
HpaII 1517 CCGG C^CGG
3846 CCGG
MspI 1517 CCGG C^CGG
3846 CCGG
NsiI 962 ATGCAT ATGCA^T
3695 ATGCAT
PflFI 1437 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1994 GACTTTGTC
PleI 2845 GACTC GAGTC(4/5)
1184 GAGTC
SspBI 842 TGTACA T^GTACA
2947 TGTACA
StyI 2564 CCAAGG C^C[AT][AT]GG
3549 CCTTGG
Tth111I 1437 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1994 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AseI AsnI Asp700I BbvI
BfaI BmyI BsaJI BsmAI Bsp1286I
BspHI BspLU11I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PshBI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI