##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AvaI AviII
BamHI BanI BbeI BbrPI BcgI
BciVI BglI BglII BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstUI Bsu36I
CelII Cfr10I CpoI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 3266 AACGTT AA^CGTT
AflII 1381 CTTAAG C^TTAAG
AhdI 2579 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2579 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2212 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2422 GGTCC G^G[AT]CC
AvrII 3008 CCTAGG C^CTAGG
BbsI 2988 GAAGAC GAAGAC(2/6)
BbvCI 3797 GCTGAGG CCTCAGC(-5/-2)
BclI 159 TGATCA T^GATCA
BcnI 2309 CCCGG CC^[GC]GG
BfrI 1381 CTTAAG C^TTAAG
BlnI 3008 CCTAGG C^CTAGG
BpuAI 2988 GAAGAC GAAGAC(2/6)
BsaBI 295 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 3541 ATCGAT AT^CGAT
Bst1107I 1049 GTATAC GTA^TAC
BstEII 3490 GGTTACC G^GT[ACGT]ACC
BstPI 3490 GGTTACC G^GT[ACGT]ACC
BstXI 3782 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1049 GTATAC GTA^TAC
CfoI 1940 GCGC GCG^C
ClaI 3541 ATCGAT AT^CGAT
Eam1105I 2579 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3490 GGTTACC G^GT[ACGT]ACC
EcoRI 3280 GAATTC G^AATTC
EcoT22I 3019 ATGCAT ATGCA^T
FauI 3803 GCGGG CCCGC(4/6)
FbaI 159 TGATCA T^GATCA
HaeII 1939 GGCGCT [GA]GCGC^[CT]
HhaI 1940 GCGC GCG^C
HinP1I 1940 GCGC G^CGC
HindIII 3244 AAGCTT A^AGCTT
MaeII 3267 ACGT A^CGT
MamI 295 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 195 CAATTG C^AATTG
MunI 195 CAATTG C^AATTG
NciI 2309 CCCGG CC^[GC]GG
NdeI 1735 CATATG CA^TATG
NsiI 3019 ATGCAT ATGCA^T
PflFI 2212 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2421 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3266 AACGTT AA^CGTT
SacI 3693 GAGCTC GAGCT^C
ScaI 2656 AGTACT AGT^ACT
SphI 3766 GCATGC GCATG^C
StuI 3515 AGGCCT AGG^CCT
SwaI 855 ATTTAAAT ATTT^AAAT
Tth111I 2212 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 2136 ACATGT A^C[GA][CT]GT
3054 ACATGT
AlwNI 1892 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
2943 CAGCAGCTG
AspHI 3289 GTGCTC G[AT]GC[AT]^C
3693 GAGCTC
BalI 1281 TGGCCA TGG^CCA
1789 TGGCCA
BsaI 828 GAGACC GGTCTC(1/5)
3904 GAGACC
BsiHKAI 3289 GTGCTC G[AT]GC[AT]^C
3693 GAGCTC
BsmFI 2826 GTCCC GGGAC(10/14)
3779 GTCCC
BspLU11I 2136 ACATGT A^CATGT
3054 ACATGT
DraII 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
2421 AGGTCCT
EaeI 1281 TGGCCA [CT]^GGCC[GA]
1789 TGGCCA
EcoO109I 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
2421 AGGTCCT
HapII 2310 CCGG C^CGG
2704 CCGG
HincII 1972 GTCAAC GT[CT]^[GA]AC
2075 GTCAAC
HindII 1972 GTCAAC GT[CT]^[GA]AC
2075 GTCAAC
HpaII 2310 CCGG C^CGG
2704 CCGG
HphI 3506 GGTGA GGTGA(8/7)
3893 GGTGA
MluNI 1281 TGGCCA TGG^CCA
1789 TGGCCA
MscI 1281 TGGCCA TGG^CCA
1789 TGGCCA
MspI 2310 CCGG C^CGG
2704 CCGG
PflMI 897 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3454 CCACAAAATGG
PstI 2949 CTGCAG CTGCA^G
3855 CTGCAG
TaqI 290 TCGA T^CGA
3542 TCGA
TthHB8I 290 TCGA T^CGA
3542 TCGA
Van91I 897 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3454 CCACAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NspI PleI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI