##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AvaI AviII AvrII
BanII BbeI BbrPI BcgI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstAPI BstUI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV FauI FseI FspI HaeII
HgaI Hin1I KasI KspI MluI
MroI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 3960 GGTACC G^GTACC
AccI 2898 GTAGAC GT^[AC][GT]AC
AflIII 1096 ACATGT A^C[GA][CT]GT
AocI 1254 CCTTAGG CC^T[ACGT]AGG
Asp700I 1434 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3960 GGTACC G^GTACC
AspI 428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1898 TGGCCA TGG^CCA
BciVI 2316 GGATAC GTATCC(6/5)
BglII 2654 AGATCT A^GATCT
BmyI 2672 GTGCCC G[AGT]GC[ACT]^C
BsaAI 1553 TACGTA [CT]AC^GT[GA]
BsaI 2684 GAGACC GGTCTC(1/5)
BsgI 3194 GTGCAG GTGCAG(16/14)
Bsp1286I 2672 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 2834 TGTACA T^GTACA
BspLU11I 1096 ACATGT A^CATGT
BsrBI 1598 GAGCGG CCGCTC(-3/-3)
BsrFI 808 GCCGGT [GA]^CCGG[CT]
BsrGI 2834 TGTACA T^GTACA
BstBI 3247 TTCGAA TT^CGAA
BstEII 1013 GGTGACC G^GT[ACGT]ACC
BstPI 1013 GGTGACC G^GT[ACGT]ACC
Bsu36I 1254 CCTTAGG CC^T[ACGT]AGG
CfoI 4213 GCGC GCG^C
Cfr10I 808 GCCGGT [GA]^CCGG[CT]
DraII 4033 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1372 CCGTGG C^C[GA][CT]GG
EaeI 1898 TGGCCA [CT]^GGCC[GA]
Eco81I 1254 CCTTAGG CC^T[ACGT]AGG
EcoO109I 4033 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1013 GGTGACC G^GT[ACGT]ACC
HapII 809 CCGG C^CGG
HhaI 4213 GCGC GCG^C
HinP1I 4213 GCGC G^CGC
HpaII 809 CCGG C^CGG
KpnI 3960 GGTACC GGTAC^C
MluNI 1898 TGGCCA TGG^CCA
MscI 1898 TGGCCA TGG^CCA
MspI 809 CCGG C^CGG
NspI 1096 ACATGT [GA]CATG^[CT]
NspV 3247 TTCGAA TT^CGAA
PacI 3023 TTAATTAA TTAAT^TAA
PflFI 428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4033 AGGACCT [GA]G^G[AT]CC[CT]
SfuI 3247 TTCGAA TT^CGAA
SnaBI 1553 TACGTA TAC^GTA
SspBI 2834 TGTACA T^GTACA
StuI 3217 AGGCCT AGG^CCT
Tth111I 428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1434 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 924 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
3444 CAGTGCCTG
BamHI 1859 GGATCC G^GATCC
2775 GGATCC
BbvCI 749 CCTCAGC CCTCAGC(-5/-2)
4244 GCTGAGG
BclI 1336 TGATCA T^GATCA
2760 TGATCA
BsaBI 1466 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3532 GATATAAATC
BspMI 466 GCAGGT ACCTGC(4/8)
709 GCAGGT
EcoT14I 959 CCAAGG C^C[AT][AT]GG
1245 CCTTGG
FbaI 1336 TGATCA T^GATCA
2760 TGATCA
MaeII 1554 ACGT A^CGT
2260 ACGT
MamI 1466 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3532 GATATAAATC
MspA1I 3267 CCGCTG C[AC]G^C[GT]G
4315 CAGCGG
PflMI 726 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4269 CCAGGAGGTGG
PleI 2716 GACTC GAGTC(4/5)
1988 GAGTC
PstI 464 CTGCAG CTGCA^G
1792 CTGCAG
SapI 1512 GAAGAGC GCTCTTC(1/4)
3088 GCTCTTC
StyI 959 CCAAGG C^C[AT][AT]GG
1245 CCTTGG
SwaI 1995 ATTTAAAT ATTT^AAAT
2992 ATTTAAAT
Van91I 726 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4269 CCAGGAGGTGG
XcmI 1653 CCAGAGGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3057 CCAGGATATTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbsI BbvI
BfaI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT22I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HpaI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MunI
MvaI MwoI NdeII NlaIII NlaIV
NsiI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII