##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccIII AclI AcsI AflII AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BsaBI BseAI
BsmI BspEI BspHI Bst1107I BstBI
BstZ17I DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 112 GACGTC GACGT^C
Acc65I 2925 GGTACC G^GTACC
AccI 3237 GTAGAC GT^[AC][GT]AC
AflIII 2762 ACATGT A^C[GA][CT]GT
AgeI 2114 ACCGGT A^CCGGT
Asp718I 2925 GGTACC G^GTACC
BalI 736 TGGCCA TGG^CCA
BlpI 1439 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1439 GCTCAGC GC^T[ACGT]AGC
BsaAI 1548 TACGTG [CT]AC^GT[GA]
BsaWI 2114 ACCGGT [AT]^CCGG[AT]
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsiWI 394 CGTACG C^GTACG
Bsp1407I 4162 TGTACA T^GTACA
BspDI 324 ATCGAT AT^CGAT
BspLU11I 2762 ACATGT A^CATGT
BsrGI 4162 TGTACA T^GTACA
BssHII 374 GCGCGC G^CGCGC
BssSI 2932 CACGAG CACGAG(-5/-1)
BstAPI 827 GCAGGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2137 GGTCACC G^GT[ACGT]ACC
BstPI 2137 GGTCACC G^GT[ACGT]ACC
CelII 1439 GCTCAGC GC^T[ACGT]AGC
ClaI 324 ATCGAT AT^CGAT
CpoI 309 CGGACCG CG^G[AT]CCG
EagI 87 CGGCCG C^GGCCG
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
EcoO65I 2137 GGTCACC G^GT[ACGT]ACC
HincII 4016 GTTAAC GT[CT]^[GA]AC
HindII 4016 GTTAAC GT[CT]^[GA]AC
HpaI 4016 GTTAAC GTT^AAC
KpnI 2925 GGTACC GGTAC^C
KspI 1259 CCGCGG CCGC^GG
MluNI 736 TGGCCA TGG^CCA
MscI 736 TGGCCA TGG^CCA
NaeI 1926 GCCGGC GCC^GGC
NcoI 2152 CCATGG C^CATGG
NgoMIV 1926 GCCGGC G^CCGGC
PinAI 2114 ACCGGT A^CCGGT
RsrII 309 CGGACCG CG^G[AT]CCG
SacII 1259 CCGCGG CCGC^GG
SexAI 3053 ACCAGGT A^CC[AT]GGT
SfiI 3131 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4162 TGTACA T^GTACA
TfiI 2733 GAATC G^A[AT]TC
XbaI 1561 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 30 GATCC GGATC(4/5)
2369 GATCC
AvrII 2426 CCTAGG C^CTAGG
4084 CCTAGG
BbvCI 2254 CCTCAGC CCTCAGC(-5/-2)
2380 CCTCAGC
BlnI 2426 CCTAGG C^CTAGG
4084 CCTAGG
BsrBI 471 GAGCGG CCGCTC(-3/-3)
3809 GAGCGG
BsrDI 926 GCAATG GCAATG(2/0)
4147 GCAATG
BsrFI 1926 GCCGGC [GA]^CCGG[CT]
2114 ACCGGT
BstXI 1543 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2026 CCAGACCCCTGG
BstYI 29 AGATCC [GA]^GATC[CT]
2368 AGATCC
Cfr10I 1926 GCCGGC [GA]^CCGG[CT]
2114 ACCGGT
DraI 1685 TTTAAA TTT^AAA
4179 TTTAAA
HindIII 1672 AAGCTT A^AGCTT
3464 AAGCTT
MflI 29 AGATCC [GA]^GATC[CT]
2368 AGATCC
SapI 1824 GCTCTTC GCTCTTC(1/4)
2358 GCTCTTC
SphI 1111 GCATGC GCATG^C
2244 GCATGC
Tsp509I 937 AATT ^AATT
4177 AATT
XhoII 29 AGATCC [GA]^GATC[CT]
2368 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XmaCI XmaI