##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HaeII
HhaI Hin1I HinP1I KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AflII 2684 CTTAAG C^TTAAG
AflIII 2410 ACGTGT A^C[GA][CT]GT
AvaII 3113 GGACC G^G[AT]CC
BanII 2463 GAGCCC G[GA]GC[CT]^C
BbsI 2978 GAAGAC GAAGAC(2/6)
BbvCI 22 CCTCAGC CCTCAGC(-5/-2)
BcnI 1705 CCGGG CC^[GC]GG
BfrI 2684 CTTAAG C^TTAAG
BpuAI 2978 GAAGAC GAAGAC(2/6)
BssSI 3764 CACGAG CACGAG(-5/-1)
EcoT14I 3535 CCTTGG C^C[AT][AT]GG
EcoT22I 1719 ATGCAT ATGCA^T
FauI 3560 GCGGG CCCGC(4/6)
HgaI 100 GCGTC GACGC(5/10)
HpaI 2176 GTTAAC GTT^AAC
NciI 1705 CCGGG CC^[GC]GG
NsiI 1719 ATGCAT ATGCA^T
PpuMI 3112 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1452 GACGGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2588 GAAGAGC GCTCTTC(1/4)
SpeI 3211 ACTAGT A^CTAGT
SphI 2085 GCATGC GCATG^C
StyI 3535 CCTTGG C^C[AT][AT]GG
XcmI 1894 CCAAATTATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1380 GTAGAC GT^[AC][GT]AC
2419 GTCTAC
Asp700I 2713 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3386 GAATTTCTTC
AspHI 3339 GTGCTC G[AT]GC[AT]^C
3564 GAGCAC
BclI 1870 TGATCA T^GATCA
4122 TGATCA
BpmI 2184 CTCCAG CTGGAG(16/14)
3624 CTCCAG
BsaAI 2409 TACGTG [CT]AC^GT[GA]
3447 TACGTG
BsaI 7 GAGACC GGTCTC(1/5)
2823 GAGACC
BsgI 2204 CTGCAC GTGCAG(16/14)
3124 GTGCAG
BsiHKAI 3339 GTGCTC G[AT]GC[AT]^C
3564 GAGCAC
BsmFI 1858 GGGAC GGGAC(10/14)
2347 GTCCC
BspHI 1867 TCATGA T^CATGA
3157 TCATGA
BstAPI 641 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2216 GCAGATCTTGC
Cfr13I 1352 GGGCC G^G[ACGT]CC
3113 GGACC
DraII 1351 AGGGCCT [GA]G^G[ACGT]CC[CT]
3112 AGGACCC
EcoNI 2788 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3104 CCTGATCTAGG
EcoO109I 1351 AGGGCCT [GA]G^G[ACGT]CC[CT]
3112 AGGACCC
FbaI 1870 TGATCA T^GATCA
4122 TGATCA
HapII 222 CCGG C^CGG
1705 CCGG
HpaII 222 CCGG C^CGG
1705 CCGG
MspI 222 CCGG C^CGG
1705 CCGG
NdeI 3956 CATATG CA^TATG
4553 CATATG
NlaIV 2462 GGAGCC GG[ACGT]^[ACGT]CC
3113 GGACCC
RcaI 1867 TCATGA T^CATGA
3157 TCATGA
Sau96I 1352 GGGCC G^G[ACGT]CC
3113 GGACC
SexAI 1903 ACCTGGT A^CC[AT]GGT
3334 ACCTGGT
StuI 834 AGGCCT AGG^CCT
3415 AGGCCT
TaqI 160 TCGA T^CGA
1372 TCGA
TthHB8I 160 TCGA T^CGA
1372 TCGA
XbaI 1817 TCTAGA T^CTAGA
1927 TCTAGA
XmnI 2713 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3386 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbvI BfaI BglII
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I DdeI DpnII
DraI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NspI PleI
PstI PvuII RsaI Sau3AI ScrFI
SfaNI SfcI SmlI SspBI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII