##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AspI BbeI
BcgI BglI BsaBI BsaHI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BstBI ClaI CpoI DrdI
Eam1105I HgaI Hin1I HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SnaBI SpeI Sse8387I SspI
Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AocI 436 CCTAAGG CC^T[ACGT]AGG
Asp700I 1578 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3229 TGCGCA TGC^GCA
AvrII 677 CCTAGG C^CTAGG
BalI 2533 TGGCCA TGG^CCA
BamHI 3071 GGATCC G^GATCC
BanI 3628 GGTGCC G^G[CT][GA]CC
BbrPI 111 CACGTG CAC^GTG
BbvCI 1907 CCTCAGC CCTCAGC(-5/-2)
BclI 4182 TGATCA T^GATCA
BglII 1241 AGATCT A^GATCT
BlnI 677 CCTAGG C^CTAGG
BlpI 381 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 381 GCTGAGC GC^T[ACGT]AGC
BsaI 775 GGTCTC GGTCTC(1/5)
Bst1107I 3148 GTATAC GTA^TAC
BstAPI 1833 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3174 CCACTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3148 GTATAC GTA^TAC
Bsu36I 436 CCTAAGG CC^T[ACGT]AGG
CelII 381 GCTGAGC GC^T[ACGT]AGC
DraIII 970 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 436 CCTAAGG CC^T[ACGT]AGG
EcoNI 177 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2458 GAATTC G^AATTC
EcoT22I 3358 ATGCAT ATGCA^T
FbaI 4182 TGATCA T^GATCA
FseI 341 GGCCGGCC GGCCGG^CC
FspI 3229 TGCGCA TGC^GCA
MluNI 2533 TGGCCA TGG^CCA
MscI 2533 TGGCCA TGG^CCA
NcoI 2845 CCATGG C^CATGG
NheI 212 GCTAGC G^CTAGC
NsiI 3358 ATGCAT ATGCA^T
PmaCI 111 CACGTG CAC^GTG
PmlI 111 CACGTG CAC^GTG
PvuII 80 CAGCTG CAG^CTG
ScaI 3916 AGTACT AGT^ACT
SgrAI 420 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 373 CCCGGG CCC^GGG
StuI 1509 AGGCCT AGG^CCT
SwaI 3474 ATTTAAAT ATTT^AAAT
XmaCI 373 CCCGGG C^CCGGG
XmaI 373 CCCGGG C^CCGGG
XmnI 1578 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3148 GTATAC GT^[AC][GT]AC
3615 GTAGAC
AflII 23 CTTAAG C^TTAAG
3980 CTTAAG
Alw44I 967 GTGCAC G^TGCAC
3807 GTGCAC
Aor51HI 4 AGCGCT AGC^GCT
324 AGCGCT
ApaI 493 GGGCCC GGGCC^C
2552 GGGCCC
ApaLI 967 GTGCAC G^TGCAC
3807 GTGCAC
AvaII 587 GGTCC G^G[AT]CC
922 GGACC
BfrI 23 CTTAAG C^TTAAG
3980 CTTAAG
BsgI 3043 CTGCAC GTGCAG(16/14)
1997 GTGCAG
BsmI 1578 GAATGC GAATGC(1/-1)
4078 GCATTC
Bsp1407I 4020 TGTACA T^GTACA
4056 TGTACA
BsrBI 616 CCGCTC CCGCTC(-3/-3)
995 CCGCTC
BsrDI 1838 CATTGC GCAATG(2/0)
1461 GCAATG
BsrGI 4020 TGTACA T^GTACA
4056 TGTACA
DraII 492 GGGGCCC [GA]G^G[ACGT]CC[CT]
2552 GGGCCCC
EagI 340 CGGCCG C^GGCCG
498 CGGCCG
EclXI 340 CGGCCG C^GGCCG
498 CGGCCG
Eco47III 4 AGCGCT AGC^GCT
324 AGCGCT
Eco52I 340 CGGCCG C^GGCCG
498 CGGCCG
EcoO109I 492 GGGGCCC [GA]G^G[ACGT]CC[CT]
2552 GGGCCCC
EcoRV 2043 GATATC GAT^ATC
2487 GATATC
HaeII 4 AGCGCT [GA]GCGC^[CT]
324 AGCGCT
HincII 2633 GTCAAC GT[CT]^[GA]AC
3445 GTCAAC
HindII 2633 GTCAAC GT[CT]^[GA]AC
3445 GTCAAC
HindIII 863 AAGCTT A^AGCTT
3350 AAGCTT
KspI 583 CCGCGG CCGC^GG
918 CCGCGG
MslI 2048 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4079 CATTCATGTG
SacI 638 GAGCTC GAGCT^C
3282 GAGCTC
SacII 583 CCGCGG CCGC^GG
918 CCGCGG
SphI 1498 GCATGC GCATG^C
3226 GCATGC
SspBI 4020 TGTACA T^GTACA
4056 TGTACA
TaqI 2041 TCGA T^CGA
2687 TCGA
TthHB8I 2041 TCGA T^CGA
2687 TCGA
XcmI 2740 CCACGATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4075 CCAGCATTCATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII