##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AflII AgeI AscI AvrII
BbeI BbvCI BclI BfrI BlnI
BsiWI BspDI BspHI BssHII Bst1107I
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoT22I FbaI FseI
HpaI KasI MfeI MunI NarI
NheI NotI NruI NsiI PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SfiI
SfoI SgrAI SnaBI StuI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3377 GACGTC GACGT^C
AccIII 1011 TCCGGA T^CCGGA
AcyI 3377 GACGTC G[GA]^CG[CT]C
AhdI 3715 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3314 GTGCAC G^TGCAC
AocI 2329 CCTGAGG CC^T[ACGT]AGG
Aor51HI 891 AGCGCT AGC^GCT
ApaLI 3314 GTGCAC G^TGCAC
AseI 4459 ATTAAT AT^TAAT
AsnI 4459 ATTAAT AT^TAAT
AspEI 3715 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4148 TGCGCA TGC^GCA
BcgI 2790 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1939 GGATAC GTATCC(6/5)
BsaBI 1297 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3377 GACGTC G[GA]^CG[CT]C
BseAI 1011 TCCGGA T^CCGGA
BspEI 1011 TCCGGA T^CCGGA
BsrBI 2875 GAGCGG CCGCTC(-3/-3)
Bsu36I 2329 CCTGAGG CC^T[ACGT]AGG
DraI 5781 TTTAAA TTT^AAA
Eam1105I 3715 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 891 AGCGCT AGC^GCT
Eco81I 2329 CCTGAGG CC^T[ACGT]AGG
EcoRI 1379 GAATTC G^AATTC
EcoRV 3487 GATATC GAT^ATC
FspI 4148 TGCGCA TGC^GCA
HgaI 3431 GCGTC GACGC(5/10)
Hin1I 3377 GACGTC G[GA]^CG[CT]C
HincII 1519 GTCAAC GT[CT]^[GA]AC
HindII 1519 GTCAAC GT[CT]^[GA]AC
KspI 4064 CCGCGG CCGC^GG
MamI 1297 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3429 ACGCGT A^CGCGT
MroI 1011 TCCGGA T^CCGGA
NaeI 3446 GCCGGC GCC^GGC
NdeI 5327 CATATG CA^TATG
NgoMIV 3446 GCCGGC G^CCGGC
PaeR7I 753 CTCGAG C^TCGAG
PshBI 4459 ATTAAT AT^TAAT
SacII 4064 CCGCGG CCGC^GG
SbfI 2160 CCTGCAGG CCTGCA^GG
ScaI 1893 AGTACT AGT^ACT
SexAI 4993 ACCTGGT A^CC[AT]GGT
SpeI 5229 ACTAGT A^CTAGT
SphI 3288 GCATGC GCATG^C
Sse8387I 2160 CCTGCAGG CCTGCA^GG
SspI 5288 AATATT AAT^ATT
XhoI 753 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2246 GTAGAC GT^[AC][GT]AC
3575 GTCTAC
ApaI 4068 GGGCCC GGGCC^C
4350 GGGCCC
AspI 683 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1586 GACATCGTC
BalI 1872 TGGCCA TGG^CCA
2469 TGGCCA
BbrPI 1514 CACGTG CAC^GTG
2045 CACGTG
BglII 1903 AGATCT A^GATCT
2451 AGATCT
BsiEI 2734 CGACCG CG[GA][CT]^CG
2800 CGACCG
BsmBI 3379 CGTCTC CGTCTC(1/5)
1355 GAGACG
BsmI 429 GCATTC GAATGC(1/-1)
1206 GCATTC
BspLU11I 3808 ACATGT A^CATGT
4703 ACATGT
BsrFI 2736 ACCGGC [GA]^CCGG[CT]
3446 GCCGGC
BstBI 357 TTCGAA TT^CGAA
797 TTCGAA
BstEII 4809 GGTAACC G^GT[ACGT]ACC
4989 GGTGACC
BstPI 4809 GGTAACC G^GT[ACGT]ACC
4989 GGTGACC
BstXI 1606 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3036 CCAAAGTCCTGG
Cfr10I 2736 ACCGGC [GA]^CCGG[CT]
3446 GCCGGC
EcoO65I 4809 GGTAACC G^GT[ACGT]ACC
4989 GGTGACC
HaeII 891 AGCGCT [GA]GCGC^[CT]
4828 AGCGCC
MluNI 1872 TGGCCA TGG^CCA
2469 TGGCCA
MscI 1872 TGGCCA TGG^CCA
2469 TGGCCA
NcoI 504 CCATGG C^CATGG
2988 CCATGG
NspV 357 TTCGAA TT^CGAA
797 TTCGAA
PflFI 683 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1586 GACATCGTC
PmaCI 1514 CACGTG CAC^GTG
2045 CACGTG
PmlI 1514 CACGTG CAC^GTG
2045 CACGTG
SfuI 357 TTCGAA TT^CGAA
797 TTCGAA
Tth111I 683 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1586 GACATCGTC
XbaI 2705 TCTAGA T^CTAGA
4285 TCTAGA
XcmI 639 CCAAAGAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
961 CCAAAACTACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I Asp718I AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI