##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AviII BciVI
BfrI BglII BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BstBI BstEII BstPI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FseI FspI
HincII HindII HpaI KpnI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Aor51HI 4531 AGCGCT AGC^GCT
Asp700I 2963 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5093 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1913 CCTAGG C^CTAGG
BbeI 4976 GGCGCC GGCGC^C
BbrPI 1731 CACGTG CAC^GTG
BbsI 3116 GTCTTC GAAGAC(2/6)
BcgI 3425 CGACTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1913 CCTAGG C^CTAGG
BpuAI 3116 GTCTTC GAAGAC(2/6)
BsaBI 3820 GATGCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4393 TCCGGT [AT]^CCGG[AT]
BspHI 578 TCATGA T^CATGA
Bst1107I 816 GTATAC GTA^TAC
BstZ17I 816 GTATAC GTA^TAC
DraIII 1204 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4531 AGCGCT AGC^GCT
EcoT22I 1865 ATGCAT ATGCA^T
HindIII 317 AAGCTT A^AGCTT
KasI 4976 GGCGCC G^GCGCC
KspI 1022 CCGCGG CCGC^GG
MamI 3820 GATGCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4976 GGCGCC GG^CGCC
NheI 370 GCTAGC G^CTAGC
NsiI 1865 ATGCAT ATGCA^T
PflFI 5093 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1731 CACGTG CAC^GTG
PmlI 1731 CACGTG CAC^GTG
PshAI 1461 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 578 TCATGA T^CATGA
SacII 1022 CCGCGG CCGC^GG
SapI 4761 GAAGAGC GCTCTTC(1/4)
SbfI 2332 CCTGCAGG CCTGCA^GG
SfoI 4976 GGCGCC GGC^GCC
SmaI 3284 CCCGGG CCC^GGG
Sse8387I 2332 CCTGCAGG CCTGCA^GG
Tth111I 5093 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3284 CCCGGG C^CCGGG
XmaI 3284 CCCGGG C^CCGGG
XmnI 2963 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 816 GTATAC GT^[AC][GT]AC
1318 GTAGAC
Alw44I 2703 GTGCAC G^TGCAC
3466 GTGCAC
ApaLI 2703 GTGCAC G^TGCAC
3466 GTGCAC
BamHI 174 GGATCC G^GATCC
506 GGATCC
BclI 632 TGATCA T^GATCA
1907 TGATCA
BlpI 374 GCTCAGC GC^T[ACGT]AGC
3652 GCTCAGC
Bpu1102I 374 GCTCAGC GC^T[ACGT]AGC
3652 GCTCAGC
BsaAI 1731 CACGTG [CT]AC^GT[GA]
1820 CACGTA
BsmI 1863 GAATGC GAATGC(1/-1)
1550 GCATTC
BspLU11I 3112 ACATGT A^CATGT
4088 ACATGT
BsrDI 4417 CATTGC GCAATG(2/0)
742 GCAATG
CelII 374 GCTCAGC GC^T[ACGT]AGC
3652 GCTCAGC
DraI 2957 TTTAAA TTT^AAA
5200 TTTAAA
FbaI 632 TGATCA T^GATCA
1907 TGATCA
HaeII 4531 AGCGCT [GA]GCGC^[CT]
4976 GGCGCC
NcoI 152 CCATGG C^CATGG
864 CCATGG
SacI 646 GAGCTC GAGCT^C
3289 GAGCTC
SphI 430 GCATGC GCATG^C
2157 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII