##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AgeI AocI AscI
Asp718I BamHI BbeI BcgI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
BstBI BstXI Bsu36I CelII ClaI
CpoI Eco81I EcoRI EcoRV FseI
HpaI KasI KpnI MamI MluI
MroI NarI NotI NruI NspV
PaeR7I PinAI PmeI PpuMI PvuI
RsrII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2537 GACGTC GACGT^C
AclI 4783 AACGTT AA^CGTT
AcyI 2537 GACGTC G[GA]^CG[CT]C
AflII 905 CTTAAG C^TTAAG
AflIII 1868 ACGTGT A^C[GA][CT]GT
AhdI 3639 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2233 GTGCAC G^TGCAC
Aor51HI 499 AGCGCT AGC^GCT
ApaI 3790 GGGCCC GGGCC^C
ApaLI 2233 GTGCAC G^TGCAC
Asp700I 1164 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3639 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3066 TGCGCA TGC^GCA
AvrII 4804 CCTAGG C^CTAGG
BanI 3740 GGCACC G^G[CT][GA]CC
BbvCI 4237 GCTGAGG CCTCAGC(-5/-2)
BciVI 3422 GTATCC GTATCC(6/5)
BclI 1026 TGATCA T^GATCA
BfrI 905 CTTAAG C^TTAAG
BglII 353 AGATCT A^GATCT
BlnI 4804 CCTAGG C^CTAGG
BsaHI 2537 GACGTC G[GA]^CG[CT]C
Bsp1407I 4745 TGTACA T^GTACA
BspHI 4708 TCATGA T^CATGA
BsrDI 4183 GCAATG GCAATG(2/0)
BsrGI 4745 TGTACA T^GTACA
Bst1107I 4152 GTATAC GTA^TAC
BstAPI 3727 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2408 GGTCACC G^GT[ACGT]ACC
BstPI 2408 GGTCACC G^GT[ACGT]ACC
BstZ17I 4152 GTATAC GTA^TAC
DraI 1301 TTTAAA TTT^AAA
DrdI 2531 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1914 CGGCCG C^GGCCG
Eam1105I 3639 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1914 CGGCCG C^GGCCG
Eco47III 499 AGCGCT AGC^GCT
Eco52I 1914 CGGCCG C^GGCCG
EcoNI 2023 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2408 GGTCACC G^GT[ACGT]ACC
FbaI 1026 TGATCA T^GATCA
FspI 3066 TGCGCA TGC^GCA
HgaI 1470 GACGC GACGC(5/10)
Hin1I 2537 GACGTC G[GA]^CG[CT]C
MfeI 64 CAATTG C^AATTG
MunI 64 CAATTG C^AATTG
NdeI 1876 CATATG CA^TATG
NheI 2963 GCTAGC G^CTAGC
Psp1406I 4783 AACGTT AA^CGTT
RcaI 4708 TCATGA T^CATGA
SacI 3038 GAGCTC GAGCT^C
ScaI 1562 AGTACT AGT^ACT
SspBI 4745 TGTACA T^GTACA
SspI 49 AATATT AAT^ATT
StuI 3442 AGGCCT AGG^CCT
XbaI 3410 TCTAGA T^CTAGA
XmnI 1164 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4152 GTATAC GT^[AC][GT]AC
4246 GTCTAC
AseI 3314 ATTAAT AT^TAAT
4011 ATTAAT
AsnI 3314 ATTAAT AT^TAAT
4011 ATTAAT
AspI 1985 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2639 GACTTTGTC
BsiEI 1723 CGACCG CG[GA][CT]^CG
1914 CGGCCG
BsmBI 402 GAGACG CGTCTC(1/5)
1468 GAGACG
BsrBI 2200 CCGCTC CCGCTC(-3/-3)
2669 GAGCGG
BsrFI 567 GCCGGC [GA]^CCGG[CT]
2660 GCCGGC
BssSI 1976 CACGAG CACGAG(-5/-1)
2375 CTCGTG
Cfr10I 567 GCCGGC [GA]^CCGG[CT]
2660 GCCGGC
DraII 529 GGGGCCT [GA]G^G[ACGT]CC[CT]
3790 GGGCCCT
DraIII 2471 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4035 CACACTGTG
EcoO109I 529 GGGGCCT [GA]G^G[ACGT]CC[CT]
3790 GGGCCCT
HincII 1718 GTCAAC GT[CT]^[GA]AC
4599 GTTGAC
HindII 1718 GTCAAC GT[CT]^[GA]AC
4599 GTTGAC
NaeI 567 GCCGGC GCC^GGC
2660 GCCGGC
NcoI 2322 CCATGG C^CATGG
3303 CCATGG
NgoMIV 567 GCCGGC G^CCGGC
2660 GCCGGC
PflFI 1985 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2639 GACTTTGTC
PflMI 1639 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2397 CCATCTGCTGG
PshAI 694 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1830 GACACTGGTC
PshBI 3314 ATTAAT AT^TAAT
4011 ATTAAT
PstI 2486 CTGCAG CTGCA^G
4174 CTGCAG
SexAI 3367 ACCAGGT A^CC[AT]GGT
4082 ACCAGGT
SmaI 607 CCCGGG CCC^GGG
3024 CCCGGG
SpeI 130 ACTAGT A^CTAGT
4265 ACTAGT
Tth111I 1985 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2639 GACTTTGTC
Van91I 1639 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2397 CCATCTGCTGG
XmaCI 607 CCCGGG C^CCGGG
3024 CCCGGG
XmaI 607 CCCGGG C^CCGGG
3024 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PacI
PleI PmaCI PmlI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII