##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AhdI AocI
Aor51HI AscI Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BlnI BsaI BsiWI Bsp1407I BspDI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoT22I FseI KpnI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 221 TCCGGA T^CCGGA
AflII 3335 CTTAAG C^TTAAG
AflIII 2232 ACGTGT A^C[GA][CT]GT
AgeI 3398 ACCGGT A^CCGGT
Alw44I 1412 GTGCAC G^TGCAC
ApaI 1825 GGGCCC GGGCC^C
ApaLI 1412 GTGCAC G^TGCAC
Asp700I 2434 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 1356 GCAACCGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3335 CTTAAG C^TTAAG
BglI 2543 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 943 AGATCT A^GATCT
BsaBI 3584 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 221 TCCGGA T^CCGGA
BsiEI 2290 CGACCG CG[GA][CT]^CG
BsmBI 2022 GAGACG CGTCTC(1/5)
BspEI 221 TCCGGA T^CCGGA
BspHI 3592 TCATGA T^CATGA
BssHII 3155 GCGCGC G^CGCGC
BstEII 2615 GGTTACC G^GT[ACGT]ACC
BstPI 2615 GGTTACC G^GT[ACGT]ACC
EcoNI 2555 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2615 GGTTACC G^GT[ACGT]ACC
EcoRV 3422 GATATC GAT^ATC
HindIII 4523 AAGCTT A^AGCTT
HpaI 3509 GTTAAC GTT^AAC
KspI 1431 CCGCGG CCGC^GG
MamI 3584 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3640 CAATTG C^AATTG
MroI 221 TCCGGA T^CCGGA
MunI 3640 CAATTG C^AATTG
NcoI 2812 CCATGG C^CATGG
PaeR7I 2333 CTCGAG C^TCGAG
PflMI 2479 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3398 ACCGGT A^CCGGT
RcaI 3592 TCATGA T^CATGA
SacII 1431 CCGCGG CCGC^GG
SalI 3993 GTCGAC G^TCGAC
ScaI 1867 AGTACT AGT^ACT
TfiI 1362 GATTC G^A[AT]TC
Van91I 2479 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2333 CTCGAG C^TCGAG
XmnI 2434 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 482 AAATTT [GA]^AATT[CT]
4295 AAATTT
ApoI 482 AAATTT [GA]^AATT[CT]
4295 AAATTT
AseI 558 ATTAAT AT^TAAT
1169 ATTAAT
AsnI 558 ATTAAT AT^TAAT
1169 ATTAAT
AviII 1843 TGCGCA TGC^GCA
2739 TGCGCA
BclI 3589 TGATCA T^GATCA
4359 TGATCA
BlpI 1367 GCTGAGC GC^T[ACGT]AGC
3478 GCTCAGC
Bpu1102I 1367 GCTGAGC GC^T[ACGT]AGC
3478 GCTCAGC
BsaWI 221 TCCGGA [AT]^CCGG[AT]
3398 ACCGGT
BsmI 1262 GCATTC GAATGC(1/-1)
3521 GCATTC
BssSI 4267 CACGAG CACGAG(-5/-1)
1628 CTCGTG
BstAPI 1405 GCAAGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3977 GCATGATTTGC
CelII 1367 GCTGAGC GC^T[ACGT]AGC
3478 GCTCAGC
DrdI 1577 GACCAACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3301 GACCCCTGAGTC
FbaI 3589 TGATCA T^GATCA
4359 TGATCA
FspI 1843 TGCGCA TGC^GCA
2739 TGCGCA
NspI 340 GCATGT [GA]CATG^[CT]
3745 GCATGT
PshAI 597 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3554 GACAGTGGTC
PshBI 558 ATTAAT AT^TAAT
1169 ATTAAT
PstI 253 CTGCAG CTGCA^G
3343 CTGCAG
SacI 3199 GAGCTC GAGCT^C
3476 GAGCTC
StuI 1492 AGGCCT AGG^CCT
4119 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI