##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
Eam1105I EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MunI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3095 GTCTAC GT^[AC][GT]AC
AflIII 3957 ACATGT A^C[GA][CT]GT
Alw44I 2426 GTGCAC G^TGCAC
AocI 993 CCTGAGG CC^T[ACGT]AGG
ApaLI 2426 GTGCAC G^TGCAC
BamHI 1611 GGATCC G^GATCC
BbeI 39 GGCGCC GGCGC^C
BsiEI 165 CGGCCG CG[GA][CT]^CG
BsiWI 1877 CGTACG C^GTACG
BsmBI 434 GAGACG CGTCTC(1/5)
BspLU11I 3957 ACATGT A^CATGT
BsrDI 3075 CATTGC GCAATG(2/0)
Bsu36I 993 CCTGAGG CC^T[ACGT]AGG
DraI 3934 TTTAAA TTT^AAA
EagI 165 CGGCCG C^GGCCG
EclXI 165 CGGCCG C^GGCCG
Eco52I 165 CGGCCG C^GGCCG
Eco81I 993 CCTGAGG CC^T[ACGT]AGG
EcoT22I 3072 ATGCAT ATGCA^T
HincII 1126 GTTGAC GT[CT]^[GA]AC
HindII 1126 GTTGAC GT[CT]^[GA]AC
KasI 39 GGCGCC G^GCGCC
NarI 39 GGCGCC GG^CGCC
NcoI 760 CCATGG C^CATGG
NdeI 2067 CATATG CA^TATG
NheI 2705 GCTAGC G^CTAGC
NsiI 3072 ATGCAT ATGCA^T
PaeR7I 3032 CTCGAG C^TCGAG
PshAI 449 GACTCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1307 GAAGAGC GCTCTTC(1/4)
SfiI 637 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 39 GGCGCC GGC^GCC
SgrAI 2885 CACCGGCG C[GA]^CCGG[CT]G
XcmI 1854 CCACTTTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3032 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 2444 GGTACC G^GTACC
3177 GGTACC
AccIII 1024 TCCGGA T^CCGGA
2532 TCCGGA
Aor51HI 1549 AGCGCT AGC^GCT
2477 AGCGCT
Asp718I 2444 GGTACC G^GTACC
3177 GGTACC
AspI 479 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3245 GACGTGGTC
BlpI 968 GCTCAGC GC^T[ACGT]AGC
2572 GCTGAGC
Bpu1102I 968 GCTCAGC GC^T[ACGT]AGC
2572 GCTGAGC
BseAI 1024 TCCGGA T^CCGGA
2532 TCCGGA
BspEI 1024 TCCGGA T^CCGGA
2532 TCCGGA
BssSI 3063 CACGAG CACGAG(-5/-1)
3105 CACGAG
CelII 968 GCTCAGC GC^T[ACGT]AGC
2572 GCTGAGC
Eco47III 1549 AGCGCT AGC^GCT
2477 AGCGCT
EcoNI 217 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1734 CCTACTACAGG
EcoT14I 229 CCTTGG C^C[AT][AT]GG
760 CCATGG
KpnI 2444 GGTACC GGTAC^C
3177 GGTACC
MroI 1024 TCCGGA T^CCGGA
2532 TCCGGA
NaeI 36 GCCGGC GCC^GGC
188 GCCGGC
NgoMIV 36 GCCGGC G^CCGGC
188 GCCGGC
NspI 3070 ACATGC [GA]CATG^[CT]
3957 ACATGT
PflFI 479 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3245 GACGTGGTC
PflMI 3134 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3291 CCACCGTGTGG
SmlI 1468 CTTGAG C^T[CT][GA]AG
3032 CTCGAG
StyI 229 CCTTGG C^C[AT][AT]GG
760 CCATGG
Tsp509I 1247 AATT ^AATT
3918 AATT
Tth111I 479 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3245 GACGTGGTC
Van91I 3134 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3291 CCACCGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII XmaCI XmaI