##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccIII AclI AhdI AscI
AspEI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
FseI KspI MluI MroI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PmeI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 6894 GGTACC G^GTACC
AccI 1344 GTCTAC GT^[AC][GT]AC
AccII 4 CGCG CG^CG
Aor51HI 5296 AGCGCT AGC^GCT
ApaI 6865 GGGCCC GGGCC^C
AseI 1809 ATTAAT AT^TAAT
AsnI 1809 ATTAAT AT^TAAT
Asp718I 6894 GGTACC G^GTACC
AspI 6193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3730 CCCGAG C^[CT]CG[GA]G
AviII 3565 TGCGCA TGC^GCA
BamHI 6246 GGATCC G^GATCC
BbeI 6073 GGCGCC GGCGC^C
BclI 748 TGATCA T^GATCA
BglII 1490 AGATCT A^GATCT
BlpI 5020 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5020 GCTCAGC GC^T[ACGT]AGC
BsmBI 6031 CGTCTC CGTCTC(1/5)
BsoBI 3730 CCCGAG C^[CT]CG[GA]G
BstBI 4422 TTCGAA TT^CGAA
BstUI 4 CGCG CG^CG
CelII 5020 GCTCAGC GC^T[ACGT]AGC
Eco47III 5296 AGCGCT AGC^GCT
EcoRV 3701 GATATC GAT^ATC
FbaI 748 TGATCA T^GATCA
FspI 3565 TGCGCA TGC^GCA
HpaI 6333 GTTAAC GTT^AAC
KasI 6073 GGCGCC G^GCGCC
KpnI 6894 GGTACC GGTAC^C
MfeI 2075 CAATTG C^AATTG
MunI 2075 CAATTG C^AATTG
MvnI 4 CGCG CG^CG
NarI 6073 GGCGCC GG^CGCC
NspV 4422 TTCGAA TT^CGAA
PflFI 6193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5157 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1809 ATTAAT AT^TAAT
ScaI 3490 AGTACT AGT^ACT
SfoI 6073 GGCGCC GGC^GCC
SfuI 4422 TTCGAA TT^CGAA
SgrAI 4056 CACCGGTG C[GA]^CCGG[CT]G
SspI 4781 AATATT AAT^ATT
Tth111I 6193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6278 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 3597 GGCGTC G[GA]^CG[CT]C
6073 GGCGCC
AgeI 4057 ACCGGT A^CCGGT
4301 ACCGGT
AvrII 225 CCTAGG C^CTAGG
6404 CCTAGG
BbrPI 5666 CACGTG CAC^GTG
6646 CACGTG
BcnI 2570 CCGGG CC^[GC]GG
3547 CCGGG
BglI 2600 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5611 GCCGCAATGGC
BlnI 225 CCTAGG C^CTAGG
6404 CCTAGG
BsaAI 5666 CACGTG [CT]AC^GT[GA]
6646 CACGTG
BsaHI 3597 GGCGTC G[GA]^CG[CT]C
6073 GGCGCC
BsaWI 4057 ACCGGT [AT]^CCGG[AT]
4301 ACCGGT
BsmI 2399 GCATTC GAATGC(1/-1)
7040 GCATTC
BspLU11I 5457 ACATGT A^CATGT
6386 ACATGT
BssSI 5984 CACGAG CACGAG(-5/-1)
5590 CTCGTG
BstAPI 3127 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6979 GCAGCTTCTGC
HgaI 2 GACGC GACGC(5/10)
3598 GCGTC
Hin1I 3597 GGCGTC G[GA]^CG[CT]C
6073 GGCGCC
NciI 2570 CCGGG CC^[GC]GG
3547 CCGGG
NheI 1544 GCTAGC G^CTAGC
6967 GCTAGC
PinAI 4057 ACCGGT A^CCGGT
4301 ACCGGT
PmaCI 5666 CACGTG CAC^GTG
6646 CACGTG
PmlI 5666 CACGTG CAC^GTG
6646 CACGTG
SexAI 372 ACCAGGT A^CC[AT]GGT
5359 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BciVI BfaI BfrI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI