##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbeI BlnI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I FseI KasI
KpnI KspI MroI NarI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 7314 GTGCAC G^TGCAC
Aor51HI 3358 AGCGCT AGC^GCT
ApaLI 7314 GTGCAC G^TGCAC
AviII 3774 TGCGCA TGC^GCA
BbrPI 7231 CACGTG CAC^GTG
BglI 1287 GCCGGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 7231 CACGTG [CT]AC^GT[GA]
BssSI 5123 CACGAG CACGAG(-5/-1)
Eco47III 3358 AGCGCT AGC^GCT
EcoT22I 3494 ATGCAT ATGCA^T
FspI 3774 TGCGCA TGC^GCA
HpaI 67 GTTAAC GTT^AAC
MfeI 2340 CAATTG C^AATTG
MluI 6899 ACGCGT A^CGCGT
MunI 2340 CAATTG C^AATTG
NaeI 2063 GCCGGC GCC^GGC
NgoMIV 2063 GCCGGC G^CCGGC
NheI 5550 GCTAGC G^CTAGC
NsiI 3494 ATGCAT ATGCA^T
PmaCI 7231 CACGTG CAC^GTG
PmlI 7231 CACGTG CAC^GTG
PshAI 6716 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 6188 ACCTGGT A^CC[AT]GGT
SmaI 4776 CCCGGG CCC^GGG
XmaCI 4776 CCCGGG C^CCGGG
XmaI 4776 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 713 CGCG CG^CG
6900 CGCG
AcyI 3225 GACGCC G[GA]^CG[CT]C
4642 GACGCC
AflII 2872 CTTAAG C^TTAAG
5583 CTTAAG
AocI 3090 CCTGAGG CC^T[ACGT]AGG
7248 CCTAAGG
BanI 629 GGTGCC G^G[CT][GA]CC
5395 GGTGCC
BfrI 2872 CTTAAG C^TTAAG
5583 CTTAAG
BsaHI 3225 GACGCC G[GA]^CG[CT]C
4642 GACGCC
BsaWI 2259 TCCGGT [AT]^CCGG[AT]
4456 TCCGGT
BstUI 713 CGCG CG^CG
6900 CGCG
BstXI 5502 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6791 CCATTCCTCTGG
Bsu36I 3090 CCTGAGG CC^T[ACGT]AGG
7248 CCTAAGG
DraIII 950 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3922 CACTGTGTG
Eco81I 3090 CCTGAGG CC^T[ACGT]AGG
7248 CCTAAGG
Hin1I 3225 GACGCC G[GA]^CG[CT]C
4642 GACGCC
MvnI 713 CGCG CG^CG
6900 CGCG
SacI 3050 GAGCTC GAGCT^C
5844 GAGCTC
ScaI 2669 AGTACT AGT^ACT
5650 AGTACT
XbaI 4120 TCTAGA T^CTAGA
6267 TCTAGA
XcmI 2078 CCACAGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7272 CCAGAGCAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI