##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Alw44I ApaLI AscI Asp718I
AviII AvrII BbeI BcgI BclI
BglI BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoO65I EcoT22I FbaI FseI
FspI KasI KpnI KspI MluI
MroI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1568 GTAGAC GT^[AC][GT]AC
AflII 4299 CTTAAG C^TTAAG
AhdI 1841 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5313 AGCGCT AGC^GCT
Asp700I 1203 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1841 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3702 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2436 CACGTG CAC^GTG
BbsI 296 GAAGAC GAAGAC(2/6)
BbvCI 1580 GCTGAGG CCTCAGC(-5/-2)
BfrI 4299 CTTAAG C^TTAAG
BlpI 102 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 102 GCTAAGC GC^T[ACGT]AGC
BpuAI 296 GAAGAC GAAGAC(2/6)
BsaAI 2436 CACGTG [CT]AC^GT[GA]
BspHI 2838 TCATGA T^CATGA
BspMI 3727 GCAGGT ACCTGC(4/8)
BstAPI 1206 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 102 GCTAAGC GC^T[ACGT]AGC
DraIII 5451 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2327 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1841 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5313 AGCGCT AGC^GCT
EcoRV 4932 GATATC GAT^ATC
MfeI 2826 CAATTG C^AATTG
MunI 2826 CAATTG C^AATTG
NcoI 16 CCATGG C^CATGG
PflFI 3702 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2436 CACGTG CAC^GTG
PmlI 2436 CACGTG CAC^GTG
RcaI 2838 TCATGA T^CATGA
SacI 5059 GAGCTC GAGCT^C
ScaI 3651 AGTACT AGT^ACT
SmaI 924 CCCGGG CCC^GGG
Tth111I 3702 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 924 CCCGGG C^CCGGG
XmaI 924 CCCGGG C^CCGGG
XmnI 1203 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 879 CCTCAGG CC^T[ACGT]AGG
4628 CCTGAGG
ApaI 2759 GGGCCC GGGCC^C
4739 GGGCCC
AseI 2156 ATTAAT AT^TAAT
3206 ATTAAT
AsnI 2156 ATTAAT AT^TAAT
3206 ATTAAT
AvaI 36 CCCGAG C^[CT]CG[GA]G
924 CCCGGG
BanI 83 GGCACC G^G[CT][GA]CC
3286 GGCACC
BsaBI 476 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3170 GATGAGTATC
BsoBI 36 CCCGAG C^[CT]CG[GA]G
924 CCCGGG
Bsp1407I 3124 TGTACA T^GTACA
4464 TGTACA
BsrFI 3283 GCCGGC [GA]^CCGG[CT]
3685 GCCGGC
BsrGI 3124 TGTACA T^GTACA
4464 TGTACA
BssSI 3165 CACGAG CACGAG(-5/-1)
2882 CTCGTG
Bsu36I 879 CCTCAGG CC^T[ACGT]AGG
4628 CCTGAGG
CfoI 689 GCGC GCG^C
5314 GCGC
Cfr10I 3283 GCCGGC [GA]^CCGG[CT]
3685 GCCGGC
DsaI 16 CCATGG C^C[GA][CT]GG
3290 CCACGG
Eco81I 879 CCTCAGG CC^T[ACGT]AGG
4628 CCTGAGG
HaeII 688 GGCGCT [GA]GCGC^[CT]
5313 AGCGCT
HhaI 689 GCGC GCG^C
5314 GCGC
HinP1I 689 GCGC G^CGC
5314 GCGC
HpaI 92 GTTAAC GTT^AAC
4365 GTTAAC
MamI 476 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3170 GATGAGTATC
NaeI 3283 GCCGGC GCC^GGC
3685 GCCGGC
NgoMIV 3283 GCCGGC G^CCGGC
3685 GCCGGC
PshBI 2156 ATTAAT AT^TAAT
3206 ATTAAT
PvuII 2166 CAGCTG CAG^CTG
4199 CAGCTG
SmlI 1867 CTCAAG C^T[CT][GA]AG
4299 CTTAAG
SphI 5423 GCATGC GCATG^C
5459 GCATGC
SspBI 3124 TGTACA T^GTACA
4464 TGTACA
XcmI 175 CCAACCTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2983 CCATGACATTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BamHI BanII BbvI
BciVI BcnI BfaI BglII BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI Hin1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SpeI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII